LEADER 05560nam 22006135 450 001 9910253944403321 005 20200705134458.0 010 $a981-10-6038-X 024 7 $a10.1007/978-981-10-6038-0 035 $a(CKB)4100000001382035 035 $a(DE-He213)978-981-10-6038-0 035 $a(MiAaPQ)EBC5185121 035 $a(PPN)222225815 035 $a(EXLCZ)994100000001382035 100 $a20171207d2017 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aBiological Small Angle Scattering: Techniques, Strategies and Tips /$fedited by Barnali Chaudhuri, Inés G. Muñoz, Shuo Qian, Volker S. Urban 205 $a1st ed. 2017. 210 1$aSingapore :$cSpringer Singapore :$cImprint: Springer,$d2017. 215 $a1 online resource (X, 268 p. 87 illus., 77 illus. in color.) 225 1 $aAdvances in Experimental Medicine and Biology,$x0065-2598 ;$v1009 311 $a981-10-6037-1 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aSample and Buffer preparation for SAXS -- Considerations for sample preparation using size-exclusion chromatography -- How to analyze and present SAS data for publication -- Designing and Performing Biological Solution Small-Angle Neutron Scattering Contrast Variation Experiments on Multi-component Assemblies -- SAS-based structural modeling and model validation -- Structural Characterization of Highly Flexible Proteins by Small-Angle Scattering -- What can we learn from wide-angle solution scattering? -- SAS-based studies of protein fibrillation -- High Resolution Distance Distributions Determined by X-ray and Neutron Scattering -- A successful combination: coupling SE-HPLC with SAXS -- Applications of SANS to study membrane protein systems -- Hybrid applications of solution scattering to aid structural biology -- A practical guide to iSPOT modeling: An integrative structural biology platform -- Small angle scattering for pharmaceutical applications: From drugs to drug delivery system. 330 $aThis book provides a clear, comprehensible and up-to-date description of how Small Angle Scattering (SAS) can help structural biology researchers. SAS is an efficient technique that offers structural information on how biological macromolecules behave in solution. SAS provides distinct and complementary data for integrative structural biology approaches in combination with other widely used probes, such as X-ray crystallography, Nuclear magnetic resonance, Mass spectrometry and Cryo-electron Microscopy. The development of brilliant synchrotron small-angle X-ray scattering (SAXS) beam lines has increased the number of researchers interested in solution scattering. SAS is especially useful for studying conformational changes in proteins, highly flexible proteins, and intrinsically disordered proteins. Small-angle neutron scattering (SANS) with neutron contrast variation is ideally suited for studying multi-component assemblies as well as membrane proteins that are stabilized in surfactant micelles or vesicles. SAS is also used for studying dynamic processes of protein fibrillation in amyloid diseases, and pharmaceutical drug delivery. The combination with size-exclusion chromatography further increases the range of SAS applications. The book is written by leading experts in solution SAS methodologies. The principles and theoretical background of various SAS techniques are included, along with practical aspects that range from sample preparation to data presentation for publication. Topics covered include techniques for improving data quality and analysis, as well as different scientific applications of SAS. With abundant illustrations and practical tips, we hope the clear explanations of the principles and the reviews on the latest progresses will serve as a guide through all aspects of biological solution SAS. The scope of this book is particularly relevant for structural biology researchers who are new to SAS. Advanced users of the technique will find it helpful for exploring the diversity of solution SAS methods and applications. Chapter 3 of this book is available open access under a CC BY 4.0 license at link.springer.com. 410 0$aAdvances in Experimental Medicine and Biology,$x0065-2598 ;$v1009 606 $aProteins 606 $aMolecular biology 606 $aBiotechnology 606 $aProtein Structure$3https://scigraph.springernature.com/ontologies/product-market-codes/L14050 606 $aMolecular Medicine$3https://scigraph.springernature.com/ontologies/product-market-codes/B1700X 606 $aBiotechnology$3https://scigraph.springernature.com/ontologies/product-market-codes/C12002 615 0$aProteins. 615 0$aMolecular biology. 615 0$aBiotechnology. 615 14$aProtein Structure. 615 24$aMolecular Medicine. 615 24$aBiotechnology. 676 $a537.5352 702 $aChaudhuri$b Barnali$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aMuñoz$b Inés G$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aQian$b Shuo$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aUrban$b Volker S$4edt$4http://id.loc.gov/vocabulary/relators/edt 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910253944403321 996 $aBiological Small Angle Scattering: Techniques, Strategies and Tips$92264739 997 $aUNINA