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010   $a3-319-58518-5
024 7 $a10.1007/978-3-319-58518-5
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035   $a(DE-He213)978-3-319-58518-5
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035   $a(PPN)201472783
035   $a(EXLCZ)993710000001393557
100   $a20170531d2017      u|         0
101 0 $aeng
135   $aurnn|008mamaa
181   $ctxt$2rdacontent
182   $cc$2rdamedia
183   $acr$2rdacarrier
200 10$aDecoding the Antibody Repertoire $eHigh Throughput Sequencing of Multiple Transcripts from Single B Cells /$fby Brandon DeKosky
205   $a1st ed. 2017.
210  1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2017.
215   $a1 online resource (XXVIII, 87 p. 34 illus.) 
225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
311   $a3-319-58517-7 
320   $aIncludes bibliographical references at the end of each chapters and index.
327   $aBackground -- High-throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire -- In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire -- Paired VH:VL Analysis of Naïve B Cell Repertoires and Comparison to Antigen-Experienced B Cell Repertoires in Healthy Human Donors -- Conclusions and Future Perspectives -- Appendices.
330   $aThis thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening the door for the discovery of new antibodies and the investigation of adaptive immune responses to vaccines and diseases. By designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells, the author directly addresses the limitations to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. Previous methods for high-throughput immune repertoire sequencing have been unable to provide such information. The techniques developed in this thesis have enabled comprehensive investigation of human B-cell repertoires and have been applied for the rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.
410  0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053
606   $aAntibodies
606   $aGenetic engineering
606   $aHuman genetics
606   $aCell biology
606   $aBiochemistry
606   $aAntibodies$3https://scigraph.springernature.com/ontologies/product-market-codes/B14010
606   $aGenetic Engineering$3https://scigraph.springernature.com/ontologies/product-market-codes/C12037
606   $aHuman Genetics$3https://scigraph.springernature.com/ontologies/product-market-codes/B12008
606   $aCell Biology$3https://scigraph.springernature.com/ontologies/product-market-codes/L16008
606   $aBiochemistry, general$3https://scigraph.springernature.com/ontologies/product-market-codes/L14005
615  0$aAntibodies.
615  0$aGenetic engineering.
615  0$aHuman genetics.
615  0$aCell biology.
615  0$aBiochemistry.
615 14$aAntibodies.
615 24$aGenetic Engineering.
615 24$aHuman Genetics.
615 24$aCell Biology.
615 24$aBiochemistry, general.
676   $a616.0798
700   $aDeKosky$b Brandon$4aut$4http://id.loc.gov/vocabulary/relators/aut$0979631
801  0$bMiAaPQ
801  1$bMiAaPQ
801  2$bMiAaPQ
906   $aBOOK
912   $a9910253930203321
996   $aDecoding the Antibody Repertoire$92234127
997   $aUNINA