LEADER 04361nam 22006495 450 001 9910253929303321 005 20200703211701.0 010 $a9783319521831 024 7 $a10.1007/978-3-319-52183-1 035 $a(CKB)3710000001072449 035 $a(DE-He213)978-3-319-52183-1 035 $a(MiAaPQ)EBC4811123 035 $a(PPN)198870256 035 $a(EXLCZ)993710000001072449 100 $a20170221d2017 u| 0 101 0 $aeng 135 $aurnn#008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aChromatin Architecture$b[electronic resource] $eAdvances From High-resolution Single Molecule DNA Imaging /$fby Kirti Prakash 205 $a1st ed. 2017. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2017. 215 $a1 online resource (LIII, 152 p. 101 illus., 50 illus. in color.) 225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 300 $a"Doctoral Thesis accepted by Institute of Molecular Biology, Mainz and Heidelberg University, Germany." 311 $a3-319-52182-9 311 $a3-319-52183-7 320 $aIncludes bibliographical references at the end of each chapters. 327 $aA Condensed History of Chromatin Research -- Investigating Chromatin Organisation Using Single Molecule Localisation Microscopy -- Structure, Function and Dynamics of Chromatin -- Periodic and Symmetric Organisation of Meiotic Chromosomes -- Conclusions -- Appendices. 330 $aThis book sheds new light on the current state of knowledge concerning chromatin organization. Particular emphasis is given to the new imaging potential offered by super-resolution microscopy, which allows DNA imaging with a very high labeling density. From the early work on chromosomes by Walther Flemming in the nineteenth century to recent advances in genomics, the history of chromatin research now spans more than a century. The various milestones, such as the discovery of the double helix structure, the sequencing of the human genome, and the recent description of the genome in 3D space, show that understanding chromatin and chromosome function requires a clear understanding of its structure. Presenting cutting-edge data from super-resolution single molecule microscopy, the book demonstrates that chromatin manifests several levels of folding, from nucleosomes to chromosomes. Chromatin domains emerge as a new fundamental building block of chromatin architecture, with functions possibly related to gene regulation. A detailed description of chromatin folding in the pachytene stage of meiosis serves as a model for exploring this functionality, showing the apparent interplay between structure, function, and epigenetic regulation. Lastly, the book discusses possible new avenues of innovation to describe chromatin?s organization and functions. Gathering essential insights on chromatin architecture, the book offers students an introduction to microscopy and its application to chromatin organization, while also providing advanced readers with new ideas for future research. 410 0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5053 606 $aCell biology 606 $aSpectroscopy 606 $aMicroscopy 606 $aNucleic acids 606 $aCell Biology$3https://scigraph.springernature.com/ontologies/product-market-codes/L16008 606 $aSpectroscopy and Microscopy$3https://scigraph.springernature.com/ontologies/product-market-codes/P31090 606 $aNucleic Acid Chemistry$3https://scigraph.springernature.com/ontologies/product-market-codes/L14011 606 $aSpectroscopy/Spectrometry$3https://scigraph.springernature.com/ontologies/product-market-codes/C11020 615 0$aCell biology. 615 0$aSpectroscopy. 615 0$aMicroscopy. 615 0$aNucleic acids. 615 14$aCell Biology. 615 24$aSpectroscopy and Microscopy. 615 24$aNucleic Acid Chemistry. 615 24$aSpectroscopy/Spectrometry. 676 $a572.87 700 $aPrakash$b Kirti$4aut$4http://id.loc.gov/vocabulary/relators/aut$0927852 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910253929303321 996 $aChromatin Architecture$92084582 997 $aUNINA