LEADER 05539nam 22005535 450 001 9910253889603321 005 20200706010430.0 010 $a3-319-27216-0 024 7 $a10.1007/978-3-319-27216-0 035 $a(CKB)3710000000667110 035 $a(DE-He213)978-3-319-27216-0 035 $a(MiAaPQ)EBC4526688 035 $a(PPN)194074323 035 $a(EXLCZ)993710000000667110 100 $a20160510d2016 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aAdvanced Technologies for Protein Complex Production and Characterization$b[electronic resource] /$fedited by M. Cristina Vega 205 $a1st ed. 2016. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2016. 215 $a1 online resource (XIII, 382 p. 87 illus., 71 illus. in color.) 225 1 $aAdvances in Experimental Medicine and Biology,$x0065-2598 ;$v896 311 $a3-319-27214-4 320 $aIncludes bibliographical references and index. 327 $aProtein complex production from the drug discovery standpoint -- Choose a suitable expression host: a survey of available protein production platforms -- ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts -- Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput -- Membrane protein production in E. coli for applications in drug discovery -- Cell-free Synthesis of Macromolecular Complexes -- A Bacillus megaterium system for the production of recombinant proteins and protein complexes -- Protein complex production in alternative prokaryotic hosts -- Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts -- Leishmania tarentolae for the Production of Multi-Subunit Complexes -- Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes -- Fundamentals of baculovirus expression and applications -- The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics -- Fundamentals of expression in mammalian cells -- Assembling multi-subunit complexes using mammalian expression -- Microalgae as solar-powered protein factories -- Strategies and methodologies for the co-expression of multiple proteins in plants -- Transient expression systems in plants?potentialities and constraints -- Complex reconstitution from individual protein modules -- Structural reconstruction of protein-protein complexes involved in intracellular signaling -- The use of small-angle scattering for the characterization of multi subunit complexes -- Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems. . 330 $aThis book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance. This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies. 410 0$aAdvances in Experimental Medicine and Biology,$x0065-2598 ;$v896 606 $aProteins  606 $aSystems biology 606 $aProtein Science$3https://scigraph.springernature.com/ontologies/product-market-codes/L14040 606 $aProtein Structure$3https://scigraph.springernature.com/ontologies/product-market-codes/L14050 606 $aSystems Biology$3https://scigraph.springernature.com/ontologies/product-market-codes/L15010 615 0$aProteins . 615 0$aSystems biology. 615 14$aProtein Science. 615 24$aProtein Structure. 615 24$aSystems Biology. 676 $a660.63 702 $aVega$b M. Cristina$4edt$4http://id.loc.gov/vocabulary/relators/edt 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910253889603321 996 $aAdvanced Technologies for Protein Complex Production and Characterization$92541158 997 $aUNINA