LEADER 01563nam0-2200481---450- 001 990000279850203316 005 19990212010757.5 010 $a0-471-12844-9 035 $a0027985 035 $aUSA010027985 035 $a(ALEPH)000027985USA01 035 $a0027985 100 $a19990212d1997----km-yaita-0103----ba 101 $aeng 102 $aUS 105 $a|||| ||||| 200 1 $aDiscrete multivariate distributions$fNorman L. Johnson, Samuel Kotz, N. Balakrishnan 210 $aNew York äOetc.]$cWiley$dcopyr. 1997 215 $aXXII, 299 p.$d24 cm 225 $aWiley series in probability and statistics. Applied probability and statistics 410 1$12001$aWiley series in probability and statistics. Applied probability and statistics 606 $aDistribuzione 676 $a519.24 700 1$aJOHNSON,$bNorman Lloyd$012699 701 1$aBALAKRISHNAN,$bNarayanaswamy$0541916 701 1$aKOTZ,$bSamuel$012052 801 0$aIT$bUNFI$c19990212 912 $a990000279850203316 951 $a519.24 JOH$b15118 Ing.$c519$d00005294 951 $a500 519.24 JOH$b9553 DISES 959 $aBK 969 $aTEC 969 $aDISES 979 $aTAMI$b40$c20001023$lUSA01$h1021 979 $c20001110$lUSA01$h1716 979 $c20020403$lUSA01$h1636 979 $aPATRY$b90$c20040406$lUSA01$h1620 979 $c20121027$lUSA01$h1541 979 $c20121027$lUSA01$h1601 979 $c20121027$lUSA01$h1610 996 $aDiscrete multivariate distributions$9880502 997 $aUNISA DEB $aUSA10100 LEADER 03180nam 2200421z- 450 001 9910220046703321 005 20210211 035 $a(CKB)3800000000216310 035 $a(oapen)https://directory.doabooks.org/handle/20.500.12854/55353 035 $a(oapen)doab55353 035 $a(EXLCZ)993800000000216310 100 $a20202102d2016 |y 0 101 0 $aeng 135 $aurmn|---annan 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aOptogenetic Tools in the Molecular Spotlight 210 $cFrontiers Media SA$d2016 215 $a1 online resource (179 p.) 225 1 $aFrontiers Research Topics 311 08$a2-88919-899-5 330 $aThe rise of optogenetics as a standard technique to non-invasively probe and monitor biological function created an immense interest in the molecular function of photosensory proteins. These photoreceptors are usually protein/pigment complexes that translate light into biological information and have become essential tools in cell biology and neurobiology as their function is genetically encoded and can be conveniently delivered into a given cell. Like for fluorescent proteins that quickly became invaluable as genetically encodable reporters in microscopy and imaging, variants of photosensory proteins with customized sensitivity and functionality are nowadays in high demand. In this ebook we feature reviews and original research on molecular approaches from synthetic biology and molecular spectroscopy to computational molecular modelling that all aspire to elucidate the molecular prerequisites for the photosensory function of the given proteins. The principle property of changing activity of biological function simply by application of light is not only very attractive for cell biology, it also offers unique opportunities for molecular studies as excitation can be controlled with high time precision. Especially in spectroscopy the usually fully reversible photoactivation of photosensory proteins allows researchers to to perform time resolved studies with up to femtosecond resolution. In addition, functional variants can be investigated and quickly screened in common biochemical experiments. The insights that are obtained by the here presented various yet complementary methods will ultimately allow us write the script for a molecular movie from excitation of the protein by a photon to activation of its biological function. Such deep understanding does not only provide unique insights into the dynamics of protein function, it will also ultimately enable us to rationally design novel optogenetic tools to be used in cell biology and therapy. 610 $acomputational modeling 610 $aFlavins 610 $aOpsins 610 $aoptogenetics 610 $aPhotochemistry and photophysics 610 $aphotoreceptors 610 $aPhytochrome 610 $aProtein Engineering 610 $aspectroscopy 700 $aJohn T. M. Kennis$4auth$01320408 702 $aTilo Mathes$4auth 906 $aBOOK 912 $a9910220046703321 996 $aOptogenetic Tools in the Molecular Spotlight$93034272 997 $aUNINA