LEADER 02834nam 2200433z- 450 001 9910220035803321 005 20231214133251.0 035 $a(CKB)3800000000216419 035 $a(oapen)https://directory.doabooks.org/handle/20.500.12854/49956 035 $a(EXLCZ)993800000000216419 100 $a20202102d2017 |y 0 101 0 $aeng 135 $aurmn|---annan 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aImaging Synapse Structure and Function 210 $cFrontiers Media SA$d2017 215 $a1 electronic resource (125 p.) 225 1 $aFrontiers Research Topics 311 $a2-88945-175-5 330 $aDevelopment of new imaging technologies in recent years has transformed neuroscience in profound ways. Following on the heels of the revolution based on the Green Fluorescent Protein, refined genetically-encoded fluorescent reporters and genetic targeting strategies now enable optical recording of synaptic transmission in defined neuronal populations at speeds approaching the enviable temporal resolution of electrophysiology. Super-resolution light microscopy permits observation of synapses and their molecular machinery at sub-diffraction resolution. At the ultrastructural level, automated forms of electron microscopy, improvements in specimen fixation methods, and recent efforts to correlate data from light and electron micrographs now make the reconstruction of functional neural circuits a reality. Finally, the use of optogenetic actuators, such as channelrhodopsins, allows precise temporal and spatial manipulation of neuronal activity and is revealing profound insights into the organization of neural circuits and their roles in behavior. This research topic highlights recent advances in both light and electron microscopy, with a specific focus on approaches that combine innovations from several different fields to obtain novel information about synapse structure and function. We are confident that this collection of articles - three original research papers, six reviews, one methods paper and one perspective article - will enable neuroscientists to achieve the next generation of experiments aimed at cracking the neural code. 610 $aconnectomics 610 $asuper-resolution 610 $aoptogenetics 610 $aSchizophrenia 610 $ametabotropic glutamate receptors 610 $abrain circuits 610 $afunctional imaging 610 $aElectron microscopy 610 $acalcium imaging 610 $aSynaptic Transmission 610 $asynaptic vesicle trafficking 700 $aGeorge J. Augustine$4auth$01331622 702 $aMarc Fivaz$4auth 906 $aBOOK 912 $a9910220035803321 996 $aImaging Synapse Structure and Function$93040507 997 $aUNINA