LEADER 03780nam 2200613Ia 450 001 9910208844503321 005 20250606201623.0 010 $a9786612686832 010 $a9780470508176 010 $a0470508175 010 $a9780470508183 010 $a0470508183 010 $a9781282686830 010 $a1282686836 010 $a9781118210390 010 $a1118210395 035 $a(CKB)4330000000003407 035 $a(MiAaPQ)EBC477712 035 $a(MiAaPQ)EBC4032843 035 $a(OCoLC)501315906 035 $a(Perlego)1009741 035 $a(EXLCZ)994330000000003407 100 $a20090210d2010 uy 0 101 0 $aeng 135 $aurcn||||||||| 181 $2rdacontent 182 $2rdamedia 183 $2rdacarrier 200 10$aIntroduction to modern liquid chromatography /$fLloyd R. Snyder, Joseph J. Kirkland, John W. Dolan 205 $a3rd ed. /$bJohn W. Dolan. 210 $aHoboken, N.J. $cWiley$dc2010 215 $axli, 912 p. $cill 311 08$a9780470167540 311 08$a0470167548 320 $aIncludes bibliographical references and index. 330 8 $aThe latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: * The basis of HPLC separation and the general effects of different experimental conditions * Equipment and detection * The column-the "heart" of the HPLC system * Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques * Computer simulation, qualitative and quantitative analysis, and method validation and quality control * The separation of large molecules, including both biological and synthetic polymers * Chiral separations, preparative separations, and sample preparation * Systematic development of HPLC separations-new to this edition * Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available. 606 $aLiquid chromatography 606 $aChromatographic analysis 606 $aCromatografia de líquids$2thub 608 $aLlibres electrònics$2thub 615 0$aLiquid chromatography. 615 0$aChromatographic analysis. 615 7$aCromatografia de líquids 676 $a543/.84 700 $aSnyder$b Lloyd R$075155 701 $aKirkland$b J. J$g(Joseph Jack),$f1925-$015270 701 $aDolan$b John W$0278317 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910208844503321 996 $aIntroduction to modern liquid chromatography$9759043 997 $aUNINA