LEADER 04990nam 22006495 450 001 9910964621303321 005 20250730113235.0 010 $a1-4757-3061-6 024 7 $a10.1007/978-1-4757-3061-6 035 $a(CKB)2660000000024304 035 $a(SSID)ssj0000932747 035 $a(PQKBManifestationID)11932792 035 $a(PQKBTitleCode)TC0000932747 035 $a(PQKBWorkID)10886541 035 $a(PQKB)11084924 035 $a(DE-He213)978-1-4757-3061-6 035 $a(MiAaPQ)EBC3085922 035 $a(PPN)238048462 035 $a(EXLCZ)992660000000024304 100 $a20130125d1999 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt 182 $cc 183 $acr 200 10$aPrinciples of Fluorescence Spectroscopy /$fedited by Joseph R. Lakowicz 205 $a2nd ed. 1999. 210 1$aNew York, NY :$cSpringer US :$cImprint: Springer,$d1999. 215 $a1 online resource (XXV, 698 p.) 300 $aIncludes index. 311 08$a0-306-46093-9 311 08$a1-4757-3063-2 327 $a1. Introduction to Fluorescence -- 2. Instrumentation for Fluorescence Spectroscopy -- 3. Fluorophores -- 4. Time-Domain Lifetime Measurements -- 5. Frequency-Domain Lifetime Measurements -- 6. Solvent Effects on Emission Spectra -- 7. Dynamics of Solvent and Spectral Relaxation -- 8. Quenching of Fluorescence -- 9. Advanced Topics in Fluorescence Quenching -- 10. Fluorescence Anisotropy -- 11. Time-Dependent Anisotropy Decays -- 12. Advanced Anisotropy Concepts -- 13. Energy Transfer -- 14. Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers -- 15. Energy Transfer to Multiple Acceptors, in One, Two, or Three Dimensions -- 16. Protein Fluorescence -- 17. Time-Resolved Protein Fluorescence -- 18. Excited-State Reactions -- 19. Fluorescence Sensing -- 20. Long-Lifetime Metal-Ligand Complexes -- 21. DNA Technology -- 22. Phase-Sensitive and Phase-Resolved Emission Spectra -- Appendix I. Corrected Emission Spectra -- 1. ?-Carboline Derivatives as Fluorescence Standards -- 2. Corrected Emission Spectra of 9,10-Diphenylanthracene, Quinine Sulfate, and Fluorescein -- 3. Long-Wavelength Standards -- 4. Ultraviolet Standards -- 5. Additional Corrected Emission Spectra -- References -- Appendix II. Fluorescent Lifetime Standards -- 1. Nanosecond Lifetime Standards -- 2. Picosecond Lifetime Standards -- 3. Representative Frequency-Domain Intensity Decays -- 4. Time-Domain Lifetime Standards -- References -- Appendix III. Additional Reading -- 1. Lifetime Measurements -- 2. Which Molecules are Fluorescent Representative Emission Spectra, and Practical Advice -- 3. Theory of Fluorescence and Photophysics -- 4. Principles of Fluorescence Spectroscopy -- 5. Biochemical Fluorescence -- 6. Protein Fluorescence -- 7. Data Analysis and Nonlinear Least Squares -- 8. Photochemistry -- 9. Flow Cytometry.-10. Phosphorescence -- 11. Polymer Science -- 12. Fluorescence Sensing -- 13. Immunoassays -- 14. Latest Applications of Fluorescence -- 15. Infrared and NIR Fluorescence -- 16. Lasers -- 17. Fluorescence Microscopy -- 18. Metal-Ligand Complexes and Unusual Luminophores -- Answers to Problems. 330 $a`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.' from the author's Preface. 606 $aAnalytical chemistry 606 $aChemistry, Physical and theoretical 606 $aBiophysics 606 $aAnalytical Chemistry 606 $aPhysical Chemistry 606 $aBiophysics 615 0$aAnalytical chemistry. 615 0$aChemistry, Physical and theoretical. 615 0$aBiophysics. 615 14$aAnalytical Chemistry. 615 24$aPhysical Chemistry. 615 24$aBiophysics. 676 $a543 676 $a543.08584 700 $aLakowicz$b Joseph R$021246 702 $aLakowicz$b Joseph R$4edt$4http://id.loc.gov/vocabulary/relators/edt 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910964621303321 996 $aPrinciples of fluorescence spectroscopy$9126659 997 $aUNINA LEADER 01373oam 2200337z- 450 001 9910162841703321 005 20230906203136.0 010 $a1-4824-6204-4 035 $a(CKB)3710000001044518 035 $a(BIP)057044014 035 $a(VLeBooks)9781482462043 035 $a(Exl-AI)993710000001044518 035 $a(EXLCZ)993710000001044518 100 $a20210505c2017uuuu -u- - 101 0 $aeng 200 $aConozco Los Numeros / I Know Numbers 210 $cGareth Stevens Publishing LLLP 215 $a1 online resource (24 p.) 225 $aLo que conozco / What I Know 311 08$a1-4824-6203-6 330 8 $aTen toes on our feet, four bases in baseball, two wheels on a bicycle; numbers are all around! This sunny book demonstrates how readers can identify numbers in familiar objects and situations, aided by carefully selected images, and encourages them to practice number recognition in their everyday lives. Number names, quantities, and counting are among the essential skills addressed in this valuable volume. 606 $aCounting$7Generated by AI 606 $aNumbers, Negative$7Generated by AI 615 0$aCounting 615 0$aNumbers, Negative 701 $aWelzen$b Jon$01248750 701 $aRateb$b Fatima$01744347 906 $aBOOK 912 $a9910162841703321 996 $aConozco Los Numeros$94175220 997 $aUNINA