LEADER 05451nam 2200745Ia 450 001 9911020239803321 005 20230617031401.0 010 $a9786610271450 010 $a9781280271458 010 $a1280271450 010 $a9780470862179 010 $a0470862173 010 $a9780470468890 010 $a0470468890 010 $a9780470867297 010 $a0470867299 035 $a(CKB)1000000000019112 035 $a(EBL)158129 035 $a(OCoLC)53813738 035 $a(SSID)ssj0000192870 035 $a(PQKBManifestationID)11166250 035 $a(PQKBTitleCode)TC0000192870 035 $a(PQKBWorkID)10215595 035 $a(PQKB)11582930 035 $a(MiAaPQ)EBC158129 035 $a(Perlego)2763273 035 $a(EXLCZ)991000000000019112 100 $a20020814d2003 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aLiquid chromatography-mass spectrometry $ean introduction /$fRobert E. Ardrey 210 $aNew York $cJ. Wiley$d2003 215 $a1 online resource (298 p.) 225 1 $aAnalytical techniques in the sciences 300 $aDescription based upon print version of record. 311 08$a9780471498018 311 08$a0471498017 311 08$a9780471497998 311 08$a0471497991 320 $aIncludes bibliographical references and index. 327 $aLIQUID CHROMATOGRAPHY-MASS SPECTROMETRY: AN INTRODUCTION; Contents; Series Preface; Preface; Acknowledgements; Abbreviations, Acronyms and Symbols; About the Author; 1 Introduction; 1.1 What are the Advantages of Linking High Performance Liquid Chromatography with Mass Spectrometry?; 1.2 What Capabilities are Required of the Combination?; 1.3 What Problems, if Any, Have to be Addressed to Allow the LC-MS Combination to Function, and Function Effectively?; References; 2 Liquid Chromatography; 2.1 Introduction; 2.2 High Performance Liquid Chromatography; 2.2.1 Pump 327 $a2.2.2 Sample Introduction (Injector)2.2.3 Mobile Phase; 2.2.4 Stationary Phase; 2.2.5 Detectors; 2.3 Chromatographic Properties; 2.4 Identification Using High Performance Liquid Chromatography; 2.5 Quantitation Using High Performance Liquid Chromatography; 2.6 The Need for High Performance Liquid Chromatography-Mass Spectrometry; References; 3 Mass Spectrometry; 3.1 Introduction; 3.2 Ionization Methods; 3.2.1 Electron Ionization; 3.2.2 Chemical Ionization; 3.2.3 Fast-Atom Bombardment; 3.2.4 Matrix-Assisted Laser Desorption Ionization; 3.2.5 Negative Ionization; 3.3 Ion Separation 327 $a3.3.1 The Quadrupole Mass Analyser3.3.2 The (Quadrupole) Ion-Trap Mass Analyser; 3.3.3 The Double-Focusing and Tri-Sector Mass Analysers; 3.3.4 The Time-of-Flight Mass Analyser; 3.4 Tandem Mass Spectrometry (MS-MS); 3.4.1 Instrumentation; 3.4.2 Techniques; 3.5 Data Acquisition; 3.5.1 Identification; 3.5.2 Quantitation; 3.6 Processing of Mass Spectral Data; 3.6.1 The Total-Ion-Current Trace; 3.6.2 Qualitative Analysis; 3.6.3 Quantitative Analysis; 3.6.4 The Use of Tandem Mass Spectrometry; References; 4 Interface Technology; 4.1 Introduction; 4.2 The Moving-Belt Interface 327 $a4.3 The Direct-Liquid-Introduction Interface4.4 The Continuous-Flow/Frit (Dynamic) Fast-Atom-Bombardment Interface; 4.5 The Particle-Beam Interface; 4.6 The Thermospray Interface; 4.7 The Electrospray Interface; 4.7.1 The Mechanism of Electrospray Ionization; 4.7.2 Sample Types; 4.7.3 The Appearance of the Electrospray Spectrum; 4.7.4 Structural Information from Electrospray Ionization; 4.8 The Atmospheric-Pressure Chemical Ionization Interface; 4.8.1 The Mechanism of Atmospheric-Pressure Chemical Ionization; References 327 $a5 Applications of High Performance Liquid Chromatography-Mass Spectrometry5.1 Method Development; 5.1.1 The Use of Experimental Design for Method Development; 5.1.2 The Choice of Electrospray or APCI; 5.2 The Molecular Weight Determination of Biopolymers; 5.2.1 Electrospray Spectra of Co-Eluting Components; 5.2.2 The Use of Selected-Ion Monitoring to Examine the Number of Terminal Galactose Moieties on a Glycoprotein; 5.2.3 The Effect of Mobile-Phase Additives and Cone-Voltage; 5.3 Structure Determination of Biopolymers; 5.3.1 Amino Acid Sequencing of Proteins 327 $a5.3.2 The Use of Enzymes for Amino Acid Sequencing 330 $aFirst explaining the basic principles of liquid chromatography and mass spectrometry and then discussing the current applications and practical benefits of LC-MS, along with descriptions of the basic instrumentation, this title will prove to be the indispensable reference source for everyone wishing to use this increasingly important tandem technique.* First book to concentrate on principles of LC-MS* Explains principles of mass spectrometry and chromatography before moving on to LC-MS* Describes instrumental aspects of LC-MS* Discusses current applications of LC-MS and shows b 410 0$aAnalytical techniques in the sciences. 606 $aLiquid chromatography 606 $aMass spectrometry 615 0$aLiquid chromatography. 615 0$aMass spectrometry. 676 $a543.84 676 $a543/.0894 700 $aArdrey$b R. 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