LEADER 05094nam 2200577 450 001 9910149172603321 005 20170810190649.0 010 $a0-08-053934-3 035 $a(CKB)1000000000384424 035 $a(EBL)1429402 035 $a(OCoLC)862831672 035 $a(SSID)ssj0000371986 035 $a(PQKBManifestationID)12137901 035 $a(PQKBTitleCode)TC0000371986 035 $a(PQKBWorkID)10420557 035 $a(PQKB)11276286 035 $a(MiAaPQ)EBC1429402 035 $a(PPN)18256746X 035 $a(EXLCZ)991000000000384424 100 $a20151111h20002000 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aPractical capillary electrophoresis /$fRobert Weinberger 205 $a2nd ed. 210 1$aSan diego, California :$cAcademic Press,$d2000. 210 4$dİ2000 215 $a1 online resource (915 p.) 300 $aDescription based upon print version of record. 311 $a0-12-742356-7 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aCover image; Title page; Table of Contents; Copyright; Dedication; PREFACE TO THE SECOND EDITION; PREFACE TO THE FIRST EDITION; MASTER SYMBOL LIST; Chapter 1: Introduction; 1.1 ELECTROPHORESIS; 1.2 MICROCHROMATOGRAPHIC SEPARATION METHODS; 1.3 CAPILLARY ELECTROPHORESIS; 1.4 CAPILLARY ELECTROCHROMATOGRAPHY; 1.5 MICROMACHINED ELECTROPHORETIC DEVICES; 1.6 HISTORICAL PERSPECTIVE; 1.7 GENERIC HPCE SYSTEMS; 1.8 INSTRUMENTATION; 1.9 SOURCES OF INFORMATION ON HPCE; 1.10 CAPILLARY ELECTROPHORESIS: A FAMILY OF TECHNIQUES; Chapter 2: Capillary Zone Electrophoresis: Basic Concepts 327 $a2.1 ELECTRICAL CONDUCTION IN FLUID SOLUTION2.2 THE LANGUAGE OF ELECTROPHORESIS; 2.3 ELECTROENDOOSMOSIS; 2.4 EFFICIENCY; 2.5 RESOLUTION; 2.6 JOULE HEATING; 2.7 OPTIMIZING THE VOLTAGE AND TEMPERATURE; 2.8 CAPILLARY DIAMETER AND BUFFER IONIC STRENGTH; 2.9 OPTIMIZING THE CAPILLARY LENGTH; 2.10 BUFFERS; 2.11 TEMPERATURE EFFECTS; 2.12 BUFFER ADDITIVES; 2.13 CAPILLARIES; 2.14 SOURCES OF BAND BROADENING; Chapter 3: Capillary Zone Electrophoresis: Methods Development; 3.1 INTRODUCTION; 3.2 MOBILITY; 3.3 SOLUTE-WALL INTERACTIONS; 3.4 SEPARATION STRATEGIES; 3.5 SECONDARY EQUILIBRIUM 327 $a3.6 APPLICATIONS AND TECHNIQUESChapter 4: Capillary Zone Electrophoresis: Secondary Equilibrium, Micelles, Cyclodextrins, and Related Reagents; 4.1 INTRODUCTION; 4.2 MICELLES; 4.3 SEPARATION MECHANISM; 4.4 SELECTING THE ELECTROLYTE SYSTEM; 4.6 ALTERNATIVE SURFACTANT SYSTEMS; 4.7 CYCLODEXTRINS; 4.8 APPLICATIONS AND METHODS DEVELOPMENT; 4.9 CHIRAL RECOGNITION; 4.10 AFFINITY CAPILLARY ELECTROPHORESIS; Chapter 5: Capillary Isoelectric Focusing; 5.1 BASIC CONCEPTS; 5.2 SEPARATION MECHANISM; 5.3 pH GRADIENT FORMATION; 5.4 ELECTRODE BUFFER SOLUTIONS; 5.5 RESOLVING POWER; 5.6 CAPILLARIES AND REAGENTS 327 $a5.7 PERFORMING A RUN5.8 INJECTION; 5.9 FOCUSING; 5.10 MOBILIZATION; 5.11 SALT EFFECTS; 5.12 DETECTION; 5.13 APPLICATIONS; Chapter 6: Size Separations in Capillary Gels and Polymer Networks; 6.1 INTRODUCTION; 6.2 SEPARATION MECHANISM; 6.3 MATERIALS FOR SIZE SEPARATIONS; 6.4 SIZE SEPARATIONS WITH NONREPLACEABLE POLYACRYLAMIDE; 6.5 SIZE SEPARATIONS WITH REPLACEABLE AGAROSE; 6.6 INTRODUCTION TO POLYMER NETWORKS; 6.7 OPERATING CHARACTERISTICS OF POLYMER NETWORKS; 6.8 ADDITIONAL MATERIALS FOR POLYMER NETWORKS; 6.9 DETECTION; 6.10 OPERATING HINTS USING POLYMER NETWORKS 327 $a6.11 APPLICATIONS AND METHODS DEVELOPMENT6.12 REDUCING THE PROBLEM OF BIASED REPTATION; Chapter 7: Capillary Electrochromatography; 7.1 INTRODUCTION; 7.2 MODES OF CEC; 7.3 ELECTROOSMOTIC FLOW IN CEC; 7.4 EFFICIENCY OF CEC; 7.5 OPERATING CHARACTERISTICS OF PACKED CEC; 7.6 APPLICATIONS; 7.7 CEC MICROFLUIDIC DEVICES; Chapter 8: Injection; 8.1 VOLUMETRIC CONSTRAINTS ON INJECTION SIZE; 8.2 PERFORMING AN INJECTION AND A RUN; 8.3 INJECTION TECHNIQUES; 8.4 SHORT-END INJECTION; 8.5 INJECTION ARTIFACTS: PROBLEMS AND SOLUTIONS; 8.6 STACKING AND TRACE ENRICHMENT; Chapter 9: Detection 327 $a9.1 ON-CAPILLARY DETECTION 330 $aIn the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small mole 606 $aCapillary electrophoresis 615 0$aCapillary electrophoresis. 676 $a547.7/04572 700 $aWeinberger$b Robert$01233803 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910149172603321 996 $aPractical capillary electrophoresis$92865727 997 $aUNINA