LEADER 05289nam 2200673Ia 450 001 9910146399103321 005 20230721021158.0 010 $a1-282-11267-8 010 $a9786612112676 010 $a0-470-38077-2 010 $a0-470-38076-4 035 $a(CKB)1000000000748583 035 $a(EBL)433867 035 $a(OCoLC)437123427 035 $a(SSID)ssj0000353865 035 $a(PQKBManifestationID)11965110 035 $a(PQKBTitleCode)TC0000353865 035 $a(PQKBWorkID)10305795 035 $a(PQKB)11382235 035 $a(MiAaPQ)EBC433867 035 $a(Au-PeEL)EBL433867 035 $a(CaPaEBR)ebr10313687 035 $a(CaONFJC)MIL211267 035 $a(EXLCZ)991000000000748583 100 $a20080624d2009 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aAptamers in bioanalysis$b[electronic resource] /$fedited by Marco Mascini 210 $aHoboken, NJ $cWiley$dc2009 215 $a1 online resource (346 p.) 300 $aDescription based upon print version of record. 311 $a0-470-14830-6 320 $aIncludes bibliographical references and index. 327 $aAPTAMERS IN BIOANALYSIS; CONTENTS; PREFACE; CONTRIBUTORS; I INTRODUCTION; 1 APTAMERS: LIGANDS FOR ALL REASONS; 1.1 Introduction; 1.2 The Power of Selection and Aptamer Refinement; 1.3 The Chemistry Drives the Shape; 1.4 Aptaregulators; 1.5 Aptasensors; 1.6 Prospects; References; 2 SELEX AND ITS RECENT OPTIMIZATIONS; 2.1 Introduction; 2.2 Aptamers and Their Selection by SELEX; 2.3 Modifications of SELEX Technology; 2.4 Advantages and Limitations of Aptamers and Their Selection Technology; 2.5 Applications of Aptamers Being Developed for the Market; 2.6 Future Perspectives; References 327 $aII BIOSENSORS3 ELECTROCHEMICAL APTASENSORS; 3.1 Introduction; 3.2 Electrochemical Aptasensor Based on Redox-Active Aptamer Monolayers Linked to Electrodes; 3.3 Enzyme-Based Amplified Electrochemical Aptasensors; 3.4 Amplified Electrochemical Aptasensors Based on Nanoparticles; 3.5 Label-Free Electrochemical Aptasensors; 3.6 Field-Effect Transistor-Based Aptasensors; 3.7 Conclusions and Perspectives; References; 4 APTAMERS: HYBRIDS BETWEEN NATURE AND TECHNOLOGY; 4.1 Introduction; 4.2 Specific Features of Aptamers; 4.3 Electrochemical Detection of Nucleic Acids 327 $a4.4 Cytochrome c Binding by Aptamers4.5 DNA Machines and Aptamers; References; 5 DETECTION OF PROTEIN-APTAMER INTERACTIONS BY MEANS OF ELECTROCHEMICAL INDICATORS AND TRANSVERSE SHEAR MODE METHOD; 5.1 Introduction; 5.2 Immobilization of Aptamers on a Solid Support; 5.3 Detection of Aptamer-Ligand Interactions; 5.3.1 Electrochemical Methods; 5.3.2 Acoustic Methods; 5.4 Conclusions; References; 6 BIOSENSORS USING THE APTAMERIC ENZYME SUBUNIT: THE USE OF APTAMERS IN THE ALLOSTERIC CONTROL OF ENZYMES; 6.1 Aptamers as Molecular Recognition Elements of Biosensors 327 $a6.1.1 Comparing Aptamers to Antibodies6.1.2 Signaling Aptamers; 6.2 Homogeneous Sensing; 6.2.1 Biosensor Systems That Do Not Require Bound-Free Separation; 6.2.2 Aptameric Enzyme Subunit; 6.3 Evolution-mimicking Algorithm for the Improvement of Aptamers; References; 7 NANOMATERIAL-BASED LABEL-FREE APTASENSORS; 7.1 Introduction; 7.2 Label-Free Electrochemical Aptasensors; 7.3 Field-Effect Transistor-Based Aptasensors; 7.4 Label-Free Aptasensors Based on Localized Surface Plasmon Resonance; 7.5 Forthcoming Challenges and Concluding Remarks; References 327 $a8 APTAMER-BASED BIOANALYTICAL ASSAYS: AMPLIFICATION STRATEGIES8.1 Introduction; 8.2 Bioanalytical Assays Based on Aptamer-Functionalized Nanoparticles; 8.3 Aptamers and Quantum Dot-Based Assays; 8.4 Aptazymes and Aptamer-Based Machines; 8.5 Polymerase Chain Reaction as an Amplification Method in Aptamer-Based Assays; 8.6 Conclusions; References; III APPLICATIONS; 9 KINETIC CAPILLARY ELECTROPHORESIS FOR SELECTION, CHARACTERIZATION, AND ANALYTICAL UTILIZATION OF APTAMERS; 9.1 Introduction; 9.1.1 Kinetic Capillary Electrophoresis; 9.1.2 The Concept of NECEEM and ECEEM 327 $a9.2 Selection of Aptamers Using KCE Methods for Partitioning and Affinity Control 330 $aThis is the first book to detail bioanalytical technologies and methods that have been developed using aptamers in analytical, medical, environmental, and food science applications. After an introduction to aptamers, aptamer targets, and their general uses, it discusses different applications with particular attention to the comparison between aptamer-based biosensors and methods versus the corresponding immunosensors. Examples of aptamer-based diagnostic techniques include whole-cell protein profiling (proteomics) and medical diagnostics for the distinction of diseased versus healthy states. 606 $aOligonucleotides 606 $aBiochemistry 615 0$aOligonucleotides. 615 0$aBiochemistry. 676 $a572.85 686 $aCHE 808f$2stub 686 $aCHE 860f$2stub 686 $aWC 4150$2rvk 701 $aMascini$b Marco$0307560 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910146399103321 996 $aAptamers in bioanalysis$92098347 997 $aUNINA