LEADER 01472nam--2200385---450- 001 990003570940203316 005 20111005135734.0 010 $a88-14-15344-2 035 $a000357094 035 $aUSA01000357094 035 $a(ALEPH)000357094USA01 035 $a000357094 100 $a20110928d2010----km-y0itay50------ba 101 $aita 102 $aIT 105 $ay|||||||001yy 200 1 $aAlterio Spinelli$eil pensiero e l'azione per la federazione europea$fa cura di Umberto Morelli$eatti del Convegno Aspetti fondamentali del pensiero e dell'azione federalista di Altiero Spinelli$e(Torino, 6-7 dicembre 2007) 210 $aMilano$cGiuffré$d2010 225 2 $aStudi$fCentro studi sul federalismo$v13 410 0$12001$aStudi$v, 13$fCentro studi sul federalismo 606 0 $aFederalismo$xRuolo [di] Spinelli, Altiero$yEuropa$2BNCF$xAtti di congressi 676 $a321.02092$v22 702 1$aMORELLI,$bUmberto 710 12$aConvegno Aspetti fondamentali del pensiero e dell'azione federalista di Altiero Spinelli$d<2007 ;$eTorino>$0610645 801 0$aIT$bsalbc$gISBD 912 $a990003570940203316 951 $a321.020 ALT 2$b68581 G.$c321.020$d00297623 959 $aBK 969 $aECO 979 $aCHIARA$b90$c20110928$lUSA01$h1508 979 $aCHIARA$b90$c20110928$lUSA01$h1509 979 $aCHIARA$b90$c20110928$lUSA01$h1510 979 $aCHIARA$b90$c20111005$lUSA01$h1357 996 $aAlterio Spinelli$91115296 997 $aUNISA LEADER 05304nam 22006494a 450 001 9910146242503321 005 20170809173113.0 010 $a1-280-52051-5 010 $a9786610520510 010 $a3-527-60542-8 010 $a3-527-60066-3 035 $a(CKB)1000000000019321 035 $a(EBL)481393 035 $a(OCoLC)68912779 035 $a(SSID)ssj0000112223 035 $a(PQKBManifestationID)11127895 035 $a(PQKBTitleCode)TC0000112223 035 $a(PQKBWorkID)10085857 035 $a(PQKB)11686613 035 $a(MiAaPQ)EBC481393 035 $a(EXLCZ)991000000000019321 100 $a20031106d2003 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aBioNMR in drug research$b[electronic resource] /$fedited by Oliver Zerbe 210 $aWeinheim $cWiley-VCH$dc2003 215 $a1 online resource (514 p.) 225 1 $aMethods and principles in medicinal chemistry ;$vv. 16 300 $aDescription based upon print version of record. 311 $a3-527-30465-7 320 $aIncludes bibliographical references and index. 327 $aBioNMR in Drug Research; Contents; Preface; Foreword; List of Authors; List of Abbreviations; Part I: Basic Techniques; 1 Modern Methods for the Expression of Proteins in Isotopically Enriched Form; 1.1 Introduction; 1.2 Isotope-Labeled Proteins from Hydrolyzates of the Green Alga Scenedesmus obliquus; 1.2.1 Production of Isotope-Labeled Algal Hydrolyzates; 1.2.2 Adaptation of the Protein Overproducer to the Algal Medium; 1.2.3 Preparation of Homogenously Isotope-Labeled Protein by Fermentation on Algal Media; 1.2.4 Amino Acid-Type Specific Labeling 327 $a1.2.5 Mass Spectrometric Analysis of the Labeled Amino Acids1.3 Selective Labeling Schemes; 1.3.1 Reverse-Labeling Schemes; 1.3.1.1 Selective Protonation of Methyl Groups in (2)H-Labeled Proteins; 1.3.1.2 Structure Determination of Selectively Methyl Protonated Proteins; 1.3.1.3 Introducting (1)H,(12)C Aromatic Residues into Otherwise (13)C Uniformly Labeled Proteins; 1.3.1.4 Backbone-Labeled Proteins; 1.3.2 Selective (13)C Methyl Group Labeling; 1.4 Intein-Based Protein Engineering for NMR Spectroscopy; 1.4.1 Segmental Labeling of Proteins 327 $a1.4.1.1 Intein-Mediated Protein Ligation (IPL)/Expressed Protein Ligation (EPL) using the IMPACT System1.4.1.2 Reconstitution of Split Inteins; 1.4.2 Stabilizing Proteins by Intein-Mediated Backbone Cyclization; 1.4.2.1 In vitro Cyclization of Proteins; 1.4.2.2 In vivo Cyclization; 1.4.2.3 Stability Enhancement by Backbone Cyclization; 1.5 Alternatives to E. coli Expression Systems; 1.5.1 Expression Vectors; 1.5.1.1 Halobacterium salinarum; 1.5.1.2 Saccharomyces cerevisiae; 1.5.1.3 Schizosaccharomyces pombe; 1.5.1.4 Pichia pastoris; 1.5.1.5 Baculovirus; 1.5.1.6 Transient Mammalian Expression 327 $a1.5.1.7 Stable Mammalian Expression1.5.1.8 Viral Vectors; 1.5.2 Comparison of Expression Systems; 1.5.3 Isotope Labeling and NMR; 1.5.4 Target Proteins; 1.6 The Use of Cell-Free Protein Expression for NMR Analysis; 1.6.1 The Cell-Free Protein Expression Systems RTS; 1.6.2 From PCR Product to (15)N-Labeled Protein; 1.6.3 Discussion and Outlook; 1.7 References; 2 Structure Calculation Using Automated Techniques; 2.1 Introduction; 2.2 Conformational Constraints for NMR Structure Calculations; 2.2.1 Constraints from Covalent Structure; 2.2.2 Steric Repulsion 327 $a2.2.3 Distance Constraints from Nuclear Overhauser Effects2.2.4 Hydrogen Bond Distance Constraints; 2.2.5 Torsion Angle Constraints from Chemical Shifts; 2.2.6 Torsion Angle Constraints from Scalar Coupling Constants; 2.2.7 Orientation Constraints; 2.3 Structure Calculation Algorithms; 2.3.1 Simulated Annealing by Molecular Dynamics Simulation in Cartesian Space; 2.3.2 Torsion Angle Dynamics; 2.4 Automated NOESY Assignment; 2.4.1 The NOESY Assignment Problem; 2.4.2 Semi-Automatic Methods; 2.4.3 General Principles of Automatic NOESY Assignment; 2.4.4 Requirements on Input Data 327 $a2.4.5 Overview of Algorithms 330 $aThe vast progress made in the investigation of biomolecules using NMR has only recently been rewarded with the Nobel Prize for Kurt W?thrich. Edited by a former coworker of W?thrich, this book presents the theoretical background on NMR of biomolecules, plus the use of NMR techniques in determining the structures of proteins and nucleic acids. BioNMR spectroscopy offers a universal tool for examining the binding of an active substance to its target protein. Its use thereby benefits the rational development of drugs. This interaction can now be investigated in a hitherto unparalleled precisi 410 0$aMethods and principles in medicinal chemistry ;$vv. 16. 606 $aPharmaceutical chemistry 606 $aNuclear magnetic resonance spectroscopy 608 $aElectronic books. 615 0$aPharmaceutical chemistry. 615 0$aNuclear magnetic resonance spectroscopy. 676 $a615.1072 676 $a615/.19 701 $aZerbe$b Oliver$0875538 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910146242503321 996 $aBioNMR in drug research$91954865 997 $aUNINA LEADER 05219nam 22006135 450 001 9910739485203321 005 20251008143629.0 010 $a9783031333309 010 $a3031333306 024 7 $a10.1007/978-3-031-33330-9 035 $a(PPN)282189319 035 $a(MiAaPQ)EBC30697447 035 $a(Au-PeEL)EBL30697447 035 $a(DE-He213)978-3-031-33330-9 035 $a(CKB)27982357600041 035 $a(OCoLC)1394118864 035 $a(EXLCZ)9927982357600041 100 $a20230814d2023 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 14$aThe Informal Powers of Western European Presidents $eA Way out of Weakness /$fby Selena Grimaldi 205 $a1st ed. 2023. 210 1$aCham :$cSpringer International Publishing :$cImprint: Palgrave Macmillan,$d2023. 215 $a1 online resource (531 pages) 225 1 $aPalgrave Studies in Presidential Politics,$x2946-5168 311 08$aPrint version: Grimaldi, Selena The Informal Powers of Western European Presidents Cham : Springer International Publishing AG,c2023 9783031333293 327 $aChapter 1: Introduction -- Chapter 2: Who are weak Presidents? Presidents? characteristics and pathways to power -- Chapter 3: Election and Term of Weak Presidents -- Chapter 4: Constitutional Strength and the Use of Formal Powers -- Chapter 5: Presidential informal powers and their use -- Chapter 6: Informal Activism in practice: the Individual presidents? experiences -- Chapter 7: When presidents go public. An analysis of presidential major speeches. 330 $a?Grimaldi?s innovative focus on presidents? personal conditions, such as high approval ratings, prompts researchers to look beyond opportunity structures to include leadership factors when analyzing determinants for successful presidential activism. Her book will be of great interest to scholars of presidential politics beyond Europe.? ---Sophia Moestrup, Washington DC, USA ?Measuring the influence of presidents is not easy. Through an innovative comparative research design and a wide range of empirical evidence, Grimaldi shows how even constitutionally weak and indirectly-elected presidents can shape policies. This book reminds us of the need to look beyond the formal powers of politicians and is a must-read for anyone interested in presidents and their role in European governance.? ?Tapio Raunio, Professor of Political Science, Tampere University, Finland ?Elegantly combining quantitative and qualitative techniques, Grimaldi describes and explains the use of informal activism of presidents in Austria, Finland, Germany, Ireland, Italy, and Portugal. This book offers a unique contribution for our understanding of how constitutionally weak presidents can maximize their influence.? ? Carsten Anckar, Professor of Political Science, Åbo Akademi University, Finland The book explores how weak presidents directly or indirectly elected can still play a crucial role in the political arena. Weak presidents have been distinguished from strong ones by relying on the evaluation of the powers they display according to the constitution. Six Western European countries are analysed: Germany and Italy which present a consistent constitutional design (as weak presidents are elected indirectly), and Austria, Ireland, Finland and Portugal that present inconsistent constitutional design (as weak presidents are elected directly). In contrast to much of the existing comparative works on presidential powers and activism, the book emphasises the role and the use of informal powers beyond that of formal ones. In particular, a definition and a typology of informal powers are provided as well as an empirical investigation on informal presidential activism. The positive outcome of presidential informal interventions are studied by relying on an interactionist approach which combines presidency-centred as well as president centred-explanations. The book argues that when dealing with informal presidential activism the opportunity structure matters but presidential public support matters even more. Selena Grimaldi is Assistant Professor and teaches Political Science at the Department of Political Science, Communication and International Relations at the University of Macerata, Italy. . 410 0$aPalgrave Studies in Presidential Politics,$x2946-5168 606 $aEurope$xPolitics and government 606 $aComparative government 606 $aPolitical leadership 606 $aEuropean Politics 606 $aComparative Politics 606 $aPolitical Leadership 615 0$aEurope$xPolitics and government. 615 0$aComparative government. 615 0$aPolitical leadership. 615 14$aEuropean Politics. 615 24$aComparative Politics. 615 24$aPolitical Leadership. 676 $a320.94 676 $a352.23094 700 $aGrimaldi$b Selena$01424936 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910739485203321 996 $aThe Informal Powers of Western European Presidents$93554594 997 $aUNINA