LEADER 05253nam 22006134a 450 001 9910146242103321 005 20170809173116.0 010 $a1-280-52042-6 010 $a9786610520428 010 $a3-527-60533-9 010 $a3-527-60149-X 035 $a(CKB)1000000000019325 035 $a(EBL)481584 035 $a(OCoLC)68927578 035 $a(SSID)ssj0000102874 035 $a(PQKBManifestationID)11622515 035 $a(PQKBTitleCode)TC0000102874 035 $a(PQKBWorkID)10060381 035 $a(PQKB)10009578 035 $a(MiAaPQ)EBC481584 035 $a(EXLCZ)991000000000019325 100 $a20030612d2003 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aAnalysing gene expression$b[electronic resource] $ea handbook of methods: possibilities and pitfalls /$fedited by Stefan Lorkowski and Paul Cullen 210 $aWeinheim ;$aNew York $cWiley-VCH$dc2003 215 $a1 online resource (984 p.) 300 $aDescription based upon print version of record. 311 $a3-527-30488-6 320 $aIncludes bibliographical references and index. 327 $aAnalysing Gene Expression A Handbook of Methods Possibilities and Pitfalls; Foreword; Preface; Contents; List of Contributors; Volume 1; 1 Basic concepts of gene expression; 1.1 Introduction; 1.2 Basics of transcription and translation in the cell; 1.2.1 Introduction; 1.2.2 Transcription; 1.2.3 Translation; 1.2.4 Summary; 1.3 Regulation of transcription; 1.3.1 Introduction; 1.3.2 mRNA expression profiles - the transcriptome; 1.3.3 Protein expression profiles - the proteome; 1.3.4 Interaction between genes and proteins - the interactome; 1.3.5 The transcription machinery and core promoters 327 $a1.3.6 Regulatory promoters1.3.7 Enhancers; 1.3.8 Locus control regions; 1.3.9 Matrix attachment regions; 1.3.10 Insulators; 1.3.11 RIDGEs - Regions of increased gene expression; 1.3.12 Enhanceosomes; 1.3.13 Chromatin; 1.3.14 Silencer elements; 1.3.15 Transcription factors, repressors and co-repressors; 1.3.16 Epigenetics; 1.3.17 Summary and conclusions; 1.4 Post-transcriptional regulation; 1.4.1 Introduction; 1.4.2 Regulation of RNA stability and degradation; 1.4.3 Regulation of transcription elongation; 1.4.3.1 Introduction; 1.4.3.2 Regulation of transcription elongation in prokaryotes 327 $a1.4.3.3 Regulation of transcription elongation in eukaryotes1.4.3.4 Conclusions; 1.4.4 Differential/alternative pre-mRNA splicing; 1.4.5 Trans-RNA splicing; 1.4.6 Regulation of mRNA transport; 1.4.7 Directed intracellular mRNA localisation; 1.4.8 Regulation of mRNA polyadenylation; 1.4.9 Antisense RNA; 1.4.10 RNA editing; 1.4.11 Summary and conclusions; 1.5 Post-translational modification of proteins; 1.5.1 Introduction; 1.5.2 Proteolytic cleavage of proteins; 1.5.3 Acylation; 1.5.4 Prenylation; 1.5.5 Methylation; 1.5.6 Sulphation; 1.5.7 Phosphorylation; 1.5.8 Ubiquitination 327 $a1.5.9 Glycosylation1.5.10 Conclusions; 1.6 Correlation of mRNA and protein expression; 1.6.1 Introduction; 1.6.2 Levels of mRNA and protein expression: correlations and discrepancies; 1.6.3 Conclusions; 1.7 Housekeeping genes, internal and external standards; 1.7.1 What are housekeeping genes?; 1.7.2 Survey of the most important housekeeping genes; 1.7.2.1 Glyceraldehyde-3-phosphate dehydrogenase; 1.7.2.2 ?-Actin; 1.7.3 Other commonly used housekeeping genes; 1.7.3.1 Ribosomal RNA (rRNA); 1.7.4 New identified 'maintenance genes'; 1.7.5 Methods of quantification 327 $a1.7.5.1 Normalisation (via internal standards)1.7.5.2 Standard curves (via external standards); 1.7.5.3 Alternative methods (via internal standards); 1.7.6 Summary; 1.8 Classification of differential gene expression technologies; 1.8.1 Introduction; 1.8.2 From single genes to transcriptomes; 1.8.3 Methods of classification; 1.8.4 Conclusions; 1.9 Summary; 1.10 References; 2 Sample preparation and supplementary tools; 2.1 Introduction; 2.2 Preparation of cells and tissues; 2.2.1 Immunopurification of cells; 2.2.2 Differential-velocity centrifugation/countercurrent elutriation 327 $a2.2.3 Surface affinity chromatography 330 $aThis book combines the experience of 225 experts on 900 pages. Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression - the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying parti 606 $aGene expression 608 $aElectronic books. 615 0$aGene expression. 676 $a572.8/65 676 $a572.865 701 $aLorkowski$b Stefan$0149121 701 $aCullen$b Paul$cDr.$0308473 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910146242103321 996 $aAnalysing gene expression$91920454 997 $aUNINA LEADER 04955nam 2200661 450 001 9910812752403321 005 20170822144447.0 010 $a1-4704-0413-3 035 $a(CKB)3360000000464996 035 $a(EBL)3114213 035 $a(SSID)ssj0000973217 035 $a(PQKBManifestationID)11616151 035 $a(PQKBTitleCode)TC0000973217 035 $a(PQKBWorkID)10959860 035 $a(PQKB)10527399 035 $a(MiAaPQ)EBC3114213 035 $a(RPAM)13639533 035 $a(PPN)195416996 035 $a(EXLCZ)993360000000464996 100 $a20150417h20042004 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aHomotopy equivalences of 3-manifolds and deformation theory of Kleinian groups /$fRichard D. Canary, Darryl McCullough 210 1$aProvidence, Rhode Island :$cAmerican Mathematical Society,$d2004. 210 4$dİ2004 215 $a1 online resource (238 p.) 225 1 $aMemoirs of the American Mathematical Society,$x0065-9266 ;$vVolume 172, Number 812 300 $a"Volume 172, Number 812 (first of 4 numbers)." 311 $a0-8218-3549-1 320 $aIncludes bibliographical references and index. 327 $a""Contents""; ""Preface""; ""Chapter 1. Introduction""; ""1.1. Motivation""; ""1.2. The main theorems for Haken 3-manifolds""; ""1.3. The main theorems for reducible 3-manifolds""; ""1.4. Examples""; ""Chapter 2. Johannson's Characteristic Submanifold Theory""; ""2.1. Fibered 3-manifolds""; ""2.2. Boundary patterns""; ""2.3. Admissible maps and mapping class groups""; ""2.4. Essential maps and useful boundary patterns""; ""2.5. The classical theorems""; ""2.6. Exceptional fibered 3-manifolds""; ""2.7. Vertical and horizontal surfaces and maps""; ""2.8. Fiber-preserving maps"" 327 $a""2.9. The characteristic submanifold""""2.10. Examples of characteristic submanifolds""; ""2.11. The Classification Theorem""; ""2.12. Miscellaneous topological results""; ""Chapter 3. Relative Compression Bodies and Cores""; ""3.1. Relative compression bodies""; ""3.2. Minimally imbedded relative compression bodies""; ""3.3. The maximal incompressible core""; ""3.4. Normally imbedded relative compression bodies""; ""3.5. The normal core and the useful core""; ""Chapter 4. Homotopy Types""; ""4.1. Homotopy equivalences preserve usefulness""; ""4.2. Finiteness of homotopy types"" 327 $a""Chapter 5. Pared 3-Manifolds""""5.1. Definitions and basic properties""; ""5.2. The topology of pared manifolds""; ""5.3. The characteristic submanifold of a pared manifold""; ""Chapter 6. Small 3-Manifolds""; ""6.1. Small manifolds and small pared manifolds""; ""6.2. Small pared homotopy types""; ""Chapter 7. Geometrically Finite Hyperbolic 3-Manifolds""; ""7.1. Basic definitions""; ""7.2. Quasiconformal deformation theory: a review""; ""7.3. The Parameterization Theorem""; ""Chapter 8. Statements of Main Theorems""; ""8.1. Statements of Main Topological Theorems"" 327 $a""8.2. Statements of Main Hyperbolic Theorem and Corollary""""8.3. Derivation of hyperbolic results""; ""Chapter 9. The Case When There Is a Compressible Free Side""; ""9.1. Algebraic lemmas""; ""9.2. The finite-index cases""; ""9.3. The infinite-index cases""; ""Chapter 10. The Case When the Boundary Pattern Is Useful""; ""10.1. The homomorphism I??""; ""10.2. Realizing homotopy equivalences of I-bundles""; ""10.3. Realizing homotopy equivalences of Seifert-fibered manifolds""; ""10.4. Proof of Main Topological Theorem 2""; ""Chapter 11. Dehn Flips"" 327 $a""Chapter 12. Finite Index Realization For Reducible 3-Manifolds""""12.1. Homeomorphisms of connected sums""; ""12.2. Reducible 3-manifolds with compressible boundary""; ""12.3. Reducible 3-manifolds with incompressible boundary""; ""Chapter 13. Epilogue""; ""13.1. More topology""; ""13.2. More geometry""; ""Bibliography""; ""Index""; ""A""; ""B""; ""C""; ""D""; ""E""; ""F""; ""G""; ""H""; ""I""; ""J""; ""K""; ""L""; ""M""; ""N""; ""O""; ""P""; ""Q""; ""R""; ""S""; ""T""; ""U""; ""V""; ""W"" 410 0$aMemoirs of the American Mathematical Society ;$vVolume 172, Number 812. 517 3 $aHomotopy equivalences of three-manifolds and deformation theory of Kleinian groups 606 $aThree-manifolds (Topology) 606 $aHomotopy equivalences 606 $aLow-dimensional topology 606 $aKleinian groups 615 0$aThree-manifolds (Topology) 615 0$aHomotopy equivalences. 615 0$aLow-dimensional topology. 615 0$aKleinian groups. 676 $a514/.3 700 $aCanary$b Richard Douglas$0311099 702 $aMcCullough$b Darryl$f1951- 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910812752403321 996 $aHomotopy equivalences of 3-manifolds and deformation theory of Kleinian groups$93952139 997 $aUNINA