LEADER 05419nam 2200673Ia 450 001 9910146071003321 005 20170809173126.0 010 $a1-280-54166-0 010 $a9786610541669 010 $a0-471-23188-6 010 $a0-471-72198-0 035 $a(CKB)1000000000019001 035 $a(EBL)469079 035 $a(OCoLC)609847797 035 $a(SSID)ssj0000307215 035 $a(PQKBManifestationID)11244322 035 $a(PQKBTitleCode)TC0000307215 035 $a(PQKBWorkID)10244058 035 $a(PQKB)10249303 035 $a(MiAaPQ)EBC469079 035 $a(PPN)150797001 035 $a(EXLCZ)991000000000019001 100 $a20000331d2000 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aProtein sequencing and identification using tandem mass spectrometry$b[electronic resource] /$fMichael Kinter, Nicholas E. Sherman 210 $aNew York $cWiley-Interscience$dc2000 215 $a1 online resource (321 p.) 225 1 $aWiley-Interscience series on mass spectrometry 300 $aDescription based upon print version of record. 311 $a0-471-32249-0 320 $aIncludes bibliographical references and index. 327 $aPROTEIN SEQUENCING AND IDENTIFICATION USING TANDEM MASS SPECTROMETRY; CONTENTS; Series Preface; Preface; Chapter 1. An Introduction to Protein Sequencing Using Tandem Mass Spectrometry; 1.1. Introduction; 1.2. References; Chapter 2. The Primary Structure of Proteins and a Historical Overview of Protein Sequencing; 2.1. Protein and Peptide Structure; 2.2. Edman Degradation; 2.2.1. The Edman Reaction; 2.2.2. Incorporation of the Edman Degradation Reaction into Automated Protein Sequenators; 2.2.3. Edman Degradation in Proteomic Research; 2.3. Tandem Mass Spectrometry 327 $a2.3.1. A Brief History of the Application of Mass Spectrometry to Protein Sequencing2.3.2. Sequence Analysis of Peptides Using Electron Ionization Mass Spectrometry; 2.3.3. The Utilization of Fast Atom Bombardment with Tandem Mass Spectrometry to Sequence Peptides; 2.3.4. Internal Sequence Analysis of Proteins Using Electrospray Ionization-Tandem Mass Spectrometry and Matrix-Assisted Laser Desorption/Ionization-Time-of- Flight Mass Spectrometry; 2.4. Summary; 2.5. References; Chapter 3. Fundamental Mass Spectrometry; 3.1. An Overview of the Instrumentation; 3.2. Ionization Methods 327 $a3.2.1. Electrospray Ionization3.2.2. Nanospray and Microspray Ionization; 3.2.3. Matrix-Assisted Laser Desorption/ Ionization; 3.3. Mass Analyzers; 3.3.1. Fundamental Parameters of Mass Analysis; 3.3.2. Quadrupole Mass Filters (3.20); 3.3.3. Ion Trap Mass Analyzers (3.21-3.23); 3.3.4. Time-of-Flight Mass Analyzers (3.5); 3.4. Tandem Mass Spectrometry; 3.4.1. Collisionally Induced Dissociation; 3.4.2. Tandem Mass Spectrometers; 3.4.3. Types of Tandem Mass Spectrometry Experiments; 3.5. Data Systems; 3.6. Summary; 3.7. References 327 $aChapter 4. Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra4.1. Introduction; 4.2. Peptide Fragmentation Chemistry; 4.2.1. Collisionally Induced Dissociation of Peptide Ions Formed by Electrospray Ionization; 4.2.2. Fragmentation of Protonated Peptide Ions Formed by Matrix-Assisted Laser Desorption/Ionization; 4.3. Interpretation of the Product Ion Spectra of Tryptic Peptides; 4.3.1. Tabulated Values Used in the Interpretation; 4.3.2. A Strategy for the Interpretation of Product Ion Spectra of Tryptic Peptides 327 $a4.3.3. Sample Interpretation Problem Number One4.3.4. Sample Interpretation Problem Number Two; 4.3.5. A Summary of Interpretation Problems One and Two; 4.3.6. Examples of More Difficult Product Ion Spectra That Cannot Be Completely Interpreted; 4.3.7. Interpretation of Product Ion Spectra from Triply Charged Ions; 4.4. Summary; 4.5. References; Chapter 5. Basic Polyacrylamide Gel Electrophoresis; 5.1. Introduction; 5.2. The Principles of Gel Electrophoresis; 5.2.1. Protein Movement and Separation; 5.2.2. Protein Detection 327 $a5.3. The Basic Steps in a Polyacrylamide Gel Electrophoresis Experiment 330 $aHow to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman 410 0$aWiley-Interscience series on mass spectrometry. 606 $aNucleotide sequence 606 $aProteins$vIdentification 606 $aMass spectrometry 615 0$aNucleotide sequence. 615 0$aProteins 615 0$aMass spectrometry. 676 $a572.85 700 $aKinter$b Michael$0971811 701 $aSherman$b Nicholas E$0971812 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910146071003321 996 $aProtein sequencing and identification using tandem mass spectrometry$92209516 997 $aUNINA