LEADER 04301nam 22004335 450 001 9910563091503321 005 20190412143002.0 010 $a1-4939-7021-6 024 7 $a10.1007/978-1-4939-7021-6 035 $a(CKB)4340000000062474 035 $a(DE-He213)978-1-4939-7021-6 035 $a(OCoLC)1028534965 035 $a(PPN)202988481 035 $a(EXLCZ)994340000000062474 100 $a20170620d2017 u| 0 101 0 $aeng 135 $aurnn#008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$a3D Cell Culture$b[electronic resource] $eMethods and Protocols /$fedited by Zuzana Koledova 205 $a1st ed. 2017. 210 1$aNew York, NY :$cSpringer New York :$cImprint: Humana,$d2017. 215 $a1 online resource (XVI, 452 p. 114 illus., 99 illus. in color.) 225 1 $aMethods in Molecular Biology,$x1064-3745 ;$v1612 311 $a1-4939-7019-4 327 $a3D Cell Culture: An Introduction -- Preparation of Decellularized Biological Scaffolds for 3D Cell Culture -- 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix -- Hydrogel-Based In Vitro Models of Tumor Angiogenesis -- Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels -- Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions.oids -- 3d="" fluid-dynamic="" models="" multiple="" myeloma -- preparation="" three-dimensional="" full="" thickness="" skin="" equivalent -- analysis="" breast="" cell="" invasion="" using="" an="" system -- 3d="" the="" brain="" parenchyma="" metastasis="" interface="" metastasis -- 3d="" neural="" in="" dual="" hydrogel="" systems -- 3d="" micro-patterned="" hydrogels="" prepared="" by="" photomask,="" micro-needle,="" or="" soft="" lithography="" techniques -- 3d="" niche="" engineering="" via="" two-photon="" laser="" polymerization -- microfluidic-based="" generation="" collagen="" spheres="" investigate="" multicellular="" spheroid="" invasion -- high-throughput="" sphere="" formation="" culture -- high-throughput="" tumor="" recyclable="" microfluidic="" platform -- high-throughput="" platform="" mesenchymal="" anastomosed="" microvascular="" network="" living="" capillary="" networks="" and="" endothelial="" cell-lined="" channels -- human="" small="" airway-on-a-chip="" protocol -- microfluidic="" bioprinting="" heterogeneous="" tissue="" constructs -- bioprinting="" decellularized="" extracellular="" matrix="" bioink -- bioprinting="" cartilage="" peg="" hydrogel -- real-time="" cycle="" imaging="" melanoma -- revealing="" ultrastructure="" morphology="" spheroids="" electron="" microscopy -- quantitative="" phenotypic="" image="" analysis="" cultures. 330 $aThis book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures. 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