LEADER 05165nam 22006614a 450 001 9910145556503321 005 20230721031218.0 010 $a1-281-22166-X 010 $a9786611221669 010 $a0-470-19650-5 010 $a0-470-19649-1 035 $a(CKB)1000000000411121 035 $a(EBL)331452 035 $a(SSID)ssj0000219603 035 $a(PQKBManifestationID)11910735 035 $a(PQKBTitleCode)TC0000219603 035 $a(PQKBWorkID)10247213 035 $a(PQKB)10307804 035 $a(MiAaPQ)EBC331452 035 $a(Au-PeEL)EBL331452 035 $a(CaPaEBR)ebr10226922 035 $a(CaONFJC)MIL122166 035 $a(OCoLC)476130814 035 $a(EXLCZ)991000000000411121 100 $a20070507d2008 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aPeptidomics$b[electronic resource] $emethods and applications /$fedited by Mikhail Soloviev, Chris Shaw, Per Andre?n 210 $aHoboken, N.J. $cWiley-Interscience$dc2008 215 $a1 online resource (432 p.) 300 $aDescription based upon print version of record. 311 $a0-471-67781-7 320 $aIncludes bibliographical references and index. 327 $aPEPTIDOMICS; CONTENTS; Preface; Contributors; PART I STUDIES OF NATURALLY OCCURRING PEPTIDES; 1 Analysis of the Peptidomes of Amphibian Skin Granular Gland Secretions-An Integrated Functional Genomic Strategy; 1.1 Introduction; 1.2 Historical Perspective; 1.3 Contemporary Methods of Sample Acquisition; 1.4 The Integrated Functional Genomic Strategy; 1.5 How then can All Three Approaches be Integrated?; 1.6 Limitations of Each Approach; 1.7 Closing Thoughts 327 $a2 A Short History of Insect (Neuro)Peptidomics-A Personal Story of the Birth and Youth of an Excellent Model For Studying Peptidome Biology2.1 Introduction; 2.2 History; 2.3 Present (and Future): Novel Technologies, New Data. . .; 2.3.1 MALDI and (Nano)ESI TOFs and QTOFs; 2.3.2 Peptide Displays; 2.3.3 Ion Traps and FT-ICRs: Greater Variety of Instruments, Greater Variety of Sequences. . .; 2.3.4 Orbitrap; 2.3.5 FT-ICRs; 2.3.6 Combining Peptide Chemical Analysis with Histological Localization: Peptidome Mass Spectrometry Imaging; 2.4 Concluding Remarks 327 $a3 Peptidomics of Short Linear Cytolytic Peptides from Spider Venom3.1 Introduction; 3.2 Peptidomics: Identifying and Sequencing Novel AMPs; 3.3 Genomics: Unraveling the Biosynthetic Pathway; 3.4 Venomics: AMPs in the Spider Venom; 3.4.1 Structural Features; 3.4.2 Functional Features; 3.4.3 Venomic Significance; 4 Molecular Cloning Approaches to Peptidomics: The Identification of Novel cDNAs Encoding Neurotoxin-like Peptide Pools; 4.1 Introduction; 4.2 Spider Toxins-Combinatorial Peptide Libraries; 4.3 EST Cloning Approach to Cloning Peptide Families 327 $a4.4 PCR-Based Amplification and Cloning Approaches4.5 The Design and Use of Hybrid Primers for the Amplification of cDNAs Coding for Large Toxin-like Peptide Pools; 4.5.1 Multiple Alignment of the Existing Toxins and Toxin-like Sequences; 4.5.2 The Design of Hybrid Partially Degenerate Primers; 4.5.2.1 The Design of the 3 ? Region; 4.5.2.2 The Design of the 5 ? Region and the Middle Part of the Primers; 4.5.2.3 The Overall Length and Degeneracy; 4.5.2.4 Annealing Temperature Matching; 4.5.3 Optimization of the Amplification Conditions; 4.5.4 Other Experimental Details 327 $a4.5.5 High Throughout Amplification and Cloning4.5.6 Sequences Identification and Analysis; 4.6 Polypeptide Toxins-Novel Applications in Drug and Pesticide Development; 4.6.1 Analgesic Drugs; 4.6.2 Insecticides; 4.7 Concluding Remarks; 4.7.1 RACE-PCR Issues; 4.7.2 Sequence Quality Issues; 4.7.3 Conclusion; 5 Wheat Antimicrobial Peptides; 5.1 Introduction; 5.2 Materials and Methods; 5.2.1 Isolation of AMPs; 5.2.2 Reduction and Alkylation of Peptides [35]; 5.2.3 MALDI-TOF MS; 5.2.4 High-Resolution Two-Dimensional Gel Electrophoresis; 5.3 Isolation of AMPs from T. kiharae Seeds 327 $a5.4 Sequence Determination of AMPs from T. kiharae Seeds 330 $aThe definitive guide to peptidomics- a hands-on lab referenceThe first truly comprehensive book about peptidomics for protein and peptide analysis, this reference provides a detailed description of the hows and whys of peptidomics and how the techniques have evolved. With chapters contributed by leading experts, it covers naturally occurring peptides, peptidomics methods and new developments, and the peptidomics approach to biomarker discovery. Explaining both the principles and the applications, Peptidomics: Methods and Applications:*Features examples of applications in di 606 $aPeptides 606 $aProteomics 615 0$aPeptides. 615 0$aProteomics. 676 $a612/.015756 701 $aSoloviev$b Mikhail$f1965-$0955337 701 $aShaw$b Chris$f1954-$0955338 701 $aAndre?n$b Per$0955339 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910145556503321 996 $aPeptidomics$92161513 997 $aUNINA