LEADER 05615nam 22006974a 450 001 9910144639903321 005 20170810193928.0 010 $a1-280-27548-0 010 $a9786610275489 010 $a0-470-24684-7 010 $a0-471-70910-7 010 $a0-471-70915-8 035 $a(CKB)1000000000226366 035 $a(EBL)228461 035 $a(OCoLC)123239768 035 $a(SSID)ssj0000229687 035 $a(PQKBManifestationID)11185821 035 $a(PQKBTitleCode)TC0000229687 035 $a(PQKBWorkID)10172433 035 $a(PQKB)11760606 035 $a(MiAaPQ)EBC228461 035 $a(EXLCZ)991000000000226366 100 $a20040603d2005 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aProteomics today$b[electronic resource] $eprotein assessment and biomarkers using mass spectrometry, 2D electrophoresis, and microarray technology /$fMahmoud Hamdan, Pier Giorgio Righetti 210 $aHoboken, N.J. $cJohn Wiley & Sons$d2005 215 $a1 online resource (446 p.) 225 1 $aWiley-Interscience series in mass spectrometry 300 $aDescription based upon print version of record. 311 $a0-471-64817-5 320 $aIncludes bibliographical references and index. 327 $aPROTEOMICS TODAY; CONTENTS; PREFACE TO PART I; ACKNOWLEDGMENT; I INTRODUCTION TO PART I; 1 INSTRUMENTATION AND DEVELOPMENTS; 1.1 Introduction; 1.2 Ionization Techniques for Macromolecules; 1.2.1 (252)Cf Plasma Desorption Ionization; 1.2.2 Fast Atom/Ion Bombardment; 1.2.3 Type of Fragment Ions and Nomenclature; 1.3 Examples on Analytical Solutions Based on FAB-MS; 1.3.1 Detection of Abnormalities in Hemoglobin; 1.3.2 Glycoprotein Structure Determination; 1.3.3 Early Scanning Functions on Sector Machines; 1.4 Electrospray Ionization; 1.5 Matrix-Assisted Laser Desorption Ionization 327 $a1.5.1 MALDI at High Pressure1.5.2 Desorption Ionization on Silicon (DIOS); 1.5.3 Delayed Extraction; 1.6 Ion Detection; 1.6.1 Microchannel Plates (MCPs); 1.6.2 Cryogenic Detectors; 1.6.2.1 Superconducting Tunnel Junction; 1.6.2.2 Thermal Detectors; 1.7 Types of Analyzers; 1.7.1 Quadrupole Mass Filter; 1.7.2 Three-Dimensional Quadrupole Ion Trap; 1.7.3 Linear Ion Trap; 1.7.4 Time of Flight; 1.7.5 Fourier Transform Ion Cyclotron Resonance; 1.8 Hybrid Analyzers; 1.8.1 Quadrupole Time of Flight; 1.8.2 Ion Mobility-TOF; 1.8.3 Linear Ion Trap-FT-ICR; 1.8.4 Ion Trap-TOF; 1.9 Tandem Mass Spectrometry 327 $a1.9.1 Postsource Decay1.9.2 MS-MS Measurements; 1.9.3 Collisional Activation; 1.10 Current MS Instrumentation in Proteome Analyses; 1.10.1 MALDI-TOF; 1.10.2 MALDI-TOF-TOF; 1.10.3 FT-ICR-MS; 1.10.4 ION Mobility-MS; 1.11 Current MS-Based Proteomics; 1.11.1 Delivering Peptides to Ion Source; 1.11.2 Peptide Sequencing and Database Searching; 1.11.3 Peptide Mass Fingerprinting; 1.11.4 Searching with MS-MS Data; 1.11.5 Databases for MS Data Search; 1.12 Recent Achievements and Future Challenges; 1.12.1 Current Applications; 1.12.2 Signal Transduction Pathways; 1.13 Concluding Remarks; References 327 $a2 PROTEOMICS IN CANCER RESEARCH2.1 Introduction; 2.1.1 Two-Dimensional Gel Electrophoresis; 2.1.2 Surface-Enhanced Laser Desorption Ionization; 2.1.3 Protein Microarrays; 2.1.4 Getting More Than Just Simple Change in Protein Expression; 2.1.5 Laser Capture Microdissection; 2.2 Pancreatic Ductal Adenocarcinoma; 2.2.1 Analyses Based on Chip Technology; 2.2.2 SELDI Analysis of Pancreatic Ductal Adenocarcinoma; 2.2.3 Protein Profiling Following Treatment with DNA Methylation/Histone Deacetylation Inhibitors; 2.2.4 Proteomic Profiling of PDAC Following Treatment with Trichostatin A 327 $a2.2.5 Proteomic Profiling of PDAC Following Treatment with 5 ?-aza-2 ?-deoxycytidine2.3 Proteomic Analysis of Human Breast Carcinoma; 2.3.1 Two-DE Analysis in Breast Cancer; 2.3.2 Proteomic Profiling of Breast Cancer Cell Membranes; 2.3.3 Proteomic Analysis on Selected Tissue Samples; 2.4 Proteomic Profiling of Chemoresistant Cancer Cells; 2.4.1 Protein Alterations in Pancreas Carcinoma Cells Exposed to Anticancer Drug; 2.4.2 Proteomic Profiling of Cervix Squamous Cell Carcinoma Treated with Cisplatin; 2.5 Signal Pathway Profiling of Prostate Cancer 327 $a2.6 Emerging Role of Functional and Activity-Based Proteomics in Disease Understanding 330 $aThe last few years have seen an unprecedented drive toward the application of proteomics to resolving challenging biomedical and biochemical tasks. Separation techniques combined with modern mass spectrometry are playing a central role in this drive. This book discusses the increasingly important role of mass spectrometry in proteomic research, and emphasizes recent advances in the existing technology and describes the advantages and pitfalls as well.* Provides a scientifically valid method for analyzing the approximatey 500,000 proteins that are encoded in the human genome* Explains t 410 0$aWiley-Interscience series on mass spectrometry. 606 $aProteins$xSpectra 606 $aMass spectrometry 606 $aProteomics 608 $aElectronic books. 615 0$aProteins$xSpectra. 615 0$aMass spectrometry. 615 0$aProteomics. 676 $a572.6 676 $a572.636 700 $aHamdan$b Mahmoud$f1947-$0862232 701 $aRighetti$b P. G$0862233 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910144639903321 996 $aProteomics today$91924673 997 $aUNINA