LEADER 05223nam 2200649Ia 450 001 9910144561703321 005 20170925031421.0 010 $a1-281-76398-5 010 $a9786611763985 010 $a3-527-61283-1 010 $a3-527-61284-X 035 $a(CKB)1000000000377123 035 $a(EBL)482273 035 $a(OCoLC)261221906 035 $a(SSID)ssj0000222468 035 $a(PQKBManifestationID)11173248 035 $a(PQKBTitleCode)TC0000222468 035 $a(PQKBWorkID)10169544 035 $a(PQKB)11336229 035 $a(MiAaPQ)EBC482273 035 $a(EXLCZ)991000000000377123 100 $a20011203d2001 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aPlasmids for therapy and vaccination$b[electronic resource] /$fedited by M. Schleef 210 $aWeinheim ;$aNew York $cWiley-VCH$dc2001 215 $a1 online resource (308 p.) 300 $aDescription based upon print version of record. 311 $a3-527-30269-7 320 $aIncludes bibliographical references and index. 327 $aPlasmids for Therapy and Vaccination; Preface; Contents; List of Contributors; 1 The Biology of Plasmids; 1 Introduction: What are plasmids?; 2 General properties of plasmids; 2.1 Plasmid replication and its control; 2.2 The molecular basis of incompatibility; 2.3 Plasmid inheritance; 2.4 Mechanisms of plasmid spread; 2.4.1 Conjugation in gram-negative bacteria; 2.4.2 Conjugation in gram-positive bacteria; 3 Plasmid-encoded phenotypes; 3.1 Bacteriocin production and resistance; 3.2 The Ti plasmids; 3.3 Heavy metal resistance; 3.4 Other phenotypical traits 327 $a4 The clinical importance of plasmids4.1 The spread of antibiotic resistance and the evolution of multiple antibiotic resistance; 4.2 Transfer of antibiotic resistance genes; 4.3 Mechanisms of antibiotic resistance; 4.4 Bacterial virulence genes; 5 Plasmid cloning vectors; 6 Perspectives; References; 2 Structures of Plasmid DNA; 1 Introduction; 2 Topological structures of plasmids; 3 Supercoiling of DNA; 4 DNA intercalating dyes; 5 Analysis of plasmid structures; 5.1 Electron microscopy (EM); 5.2 Agarose gel electrophoresis (AGE); 5.3 Capillary gel electrophoresis (CGE) 327 $a5.4 Analytical chromatography6 Conclusion; References; 3 Genetic Vaccination with Plasmid Vectors; 1 Introduction; 2 Vector design; 2.1 Plasmid DNA; 2.2 Construction of simple transcription units; 2.3 Construction of complex transcription units; 3 Strategies for DNA delivery; 4 Priming humoral and cellular immune responses by DNA vaccines; 5 Experimental strategies facilitated by DNA vaccination; 6 Unique advantages of DNA vaccination; 7 DNA vaccines in preclinical animal models; 7.1 DNA vaccines to control infectious diseases; 7.2 Therapeutic tumor vaccines; 7.3 Autoimmune disease 327 $a7.4 Treatment of allergy by therapeutic DNA vaccination8 Proposed clinical applications of DNA vaccines; 9 Risks of nucleic acid vaccination; 10 Future perspectives; References; 4 A Liposomal iNOS-Gene Therapy Approach to Prevent Neointimal Lesion Formation in Porcine Femoral Arteries; 1 Introduction; 2 Results and discussion; 2.1 Therapeutic plasmid; 2.2 The gene therapy product has a clinically acceptable format; 2.3 Efficient gene transfer was established in a minipig femoral artery injury model; 2.4 Transfection efficiency is dose dependent 327 $a2.5 Non-viral iNOS gene transfer efficiently inhibits neointimal lesion formation3 Summary and perspectives; References; 5 lmmunotherapy of Chronic Hepatitis B by pCMV-S2.S DNA Vaccine; 1 Introduction; 1.1 Hepatitis B: the disease; 1.2 Hepatitis B: treatments; 1.3 Hepatitis B: immune response to infection; 1.4 What are DNA vaccines?; 1.5 Which DNA vaccines for hepatitis B?; 2 DNA vaccines for the prevention of hepatitis B; 2.1 The mouse model; 2.1.1 Humoral response; 2.1.2 Cell-mediated response; 2.1.3 Mechanisms of DNA-induced immune response to HBsAg; 2.1.4 The primate model 327 $a2.1.5 DNA-based vaccination of chimpanzees against HBV 330 $aThis is the first book specializing in plasmids and their biomedical use, including all relevant aspects of production, applications, quality, and regulations. Readers will discover clinical applications for the wide range of preventive and therapeutic applications using plasmid DNA. The book describes modified vector systems based on plasmids, as well as the potency of genomic research and vector design by informatics. Using the example of fish vaccination, the application of DNA vaccination in veterinary health care is reviewed, followed by a detailed overview of plasmid production technol 606 $aDNA vaccines 606 $aGene therapy 606 $aPlasmids 608 $aElectronic books. 615 0$aDNA vaccines. 615 0$aGene therapy. 615 0$aPlasmids. 676 $a615.32 676 $a615.372 701 $aSchleef$b M$g(Martin)$0872773 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910144561703321 996 $aPlasmids for therapy and vaccination$92054470 997 $aUNINA LEADER 01388nam 2200457Ia 450 001 9910782635003321 005 20230207230035.0 035 $a(CKB)1000000000713947 035 $a(SSID)ssj0000675023 035 $a(PQKBManifestationID)11378968 035 $a(PQKBTitleCode)TC0000675023 035 $a(PQKBWorkID)10668256 035 $a(PQKB)10436055 035 $a(MiAaPQ)EBC3306308 035 $a(Au-PeEL)EBL3306308 035 $a(CaPaEBR)ebr10201117 035 $a(OCoLC)870418462 035 $a(EXLCZ)991000000000713947 100 $a20060303d2006 uy 0 101 0 $aspa 135 $aurcn||||||||| 181 $ctxt 182 $cc 183 $acr 200 00$aUniversalismo basico$b[electronic resource] $euna mueva politica social para America Latina /$fCarlos Gerardo Molina, editor 210 $aWashington, DC $cInter-American Development Bank$dc2006 215 $axv, 388 p 300 $aBibliographic Level Mode of Issuance: Monograph 311 $a1-59782-034-2 606 $aSocial planning$zLatin America 606 $aHuman services$zLatin America 615 0$aSocial planning 615 0$aHuman services 701 $aMolina$b Carlos Gerardo$01499949 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910782635003321 996 $aUniversalismo basico$93726391 997 $aUNINA