LEADER 05437nam 2200637Ia 450 001 9910144559603321 005 20170814171740.0 010 $a1-280-92167-6 010 $a9786610921676 010 $a3-527-61092-8 010 $a3-527-61093-6 035 $a(CKB)1000000000377144 035 $a(EBL)481506 035 $a(OCoLC)173135610 035 $a(SSID)ssj0000156119 035 $a(PQKBManifestationID)11158482 035 $a(PQKBTitleCode)TC0000156119 035 $a(PQKBWorkID)10134196 035 $a(PQKB)10823756 035 $a(MiAaPQ)EBC481506 035 $a(EXLCZ)991000000000377144 100 $a20060529d2007 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aFlow cytometry with plant cells$b[electronic resource] $eanalysis of genes, chromosomes and genomes /$fedited by Jaroslav Dolez?el, Johann Greilhuber, and Jan Suda 210 $aWeinheim $cWiley-VCH ;$aChichester $cJohn Wiley [distributor]$d2007 215 $a1 online resource (481 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31487-3 320 $aIncludes bibliographical references and index. 327 $aFlow Cytometry with Plant Cells; Contents; Preface; List of Contributors; 1 Cytometry and Cytometers: Development and Growth; Overview; 1.1 Origins; 1.2 From Absorption to Fluorescence, from Imaging to Flow; 1.2.1 Early Microspectrophotometry and Image Cytometry; 1.2.2 Fluorescence Microscopy and the Fluorescent Antibody Technique; 1.2.3 Computers Meet Cytometers: The Birth of Analytical Flow Cytometry; 1.2.4 The Development of Cell Sorting; 1.3 The Growth of Multiparameter Flow Cytometry; 1.4 Bench-tops and Behemoths: Convergent Evolution; 1.5 Image Cytometry: New Beginnings?; References 327 $a2 Principles of Flow CytometryOverview; 2.1 Introduction; 2.2 A Brief History of Flow Cytometry; 2.3 Components of a Flow Cytometer; 2.3.1 Fluidics; 2.3.2 Optics; 2.3.3 Electronic Systems; 2.4 Flow Cytometric Informatics; 2.5 Spectral Compensation; 2.6 Cell Sorting; 2.7 Calibration Issues; 2.8 Conclusions; References; 3 Flow Cytometry with Plants: an Overview; Overview; 3.1 Introduction; 3.2 Fluorescence is a Fundamental Parameter; 3.3 Pushing Plants through the Flow Cytometer; 3.3.1 Difficulties with Plants and their Cells; 3.3.2 Protoplasts are somewhat ""Easier"" than Intact Cells 327 $a3.3.3 Going for Organelles3.4 Application of Flow Cytometry in Plants; 3.4.1 Microspores and Pollen; 3.4.2 Protoplasts; 3.4.2.1 Physiological Processes; 3.4.2.2 Secondary Metabolites; 3.4.2.3 Gene Expression; 3.4.2.4 Somatic Hybrids; 3.4.2.5 DNA Transfection; 3.4.3 Cell Nuclei; 3.4.3.1 Ploidy Levels; 3.4.3.2 Aneuploidy; 3.4.3.3 B Chromosomes; 3.4.3.4 Sex Chromosomes; 3.4.3.5 Cell Cycle and Endopolyploidy; 3.4.3.6 Reproductive Pathways; 3.4.3.7 Nuclear Genome Size; 3.4.3.8 DNA Base Content; 3.4.3.9 Chromatin Composition; 3.4.3.10 Sorting of Nuclei; 3.4.4 Mitotic Chromosomes; 3.4.5 Chloroplasts 327 $a3.4.6 Mitochondria3.4.7 Plant Pathogens; 3.4.8 Aquatic Flow Cytometry; 3.5 A Flow Cytometer in Every Laboratory?; 3.6 Conclusions and Future Trends; References; 4 Nuclear DNA Content Measurement; Overview; 4.1 Introduction; 4.2 Nuclear DNA Content: Words, Concepts and Symbols; 4.2.1 Replication-Division Phases; 4.2.2 Alternation of Nuclear Phases; 4.2.3 Generative Polyploidy Levels; 4.2.4 Somatic Polyploidy; 4.3 Units for Presenting DNA Amounts and their Conversion Factors; 4.4 Sample Preparation for Flow Cytometric DNA Measurement; 4.4.1 Selection of the Tissue; 4.4.2 Reagents and Solutions 327 $a4.4.2.1 Isolation Buffers and DNA Staining4.5 Standardization; 4.5.1 Types of Standardization; 4.5.2 Requirement of Internal Standardization - a Practical Test; 4.5.3 Choice of the Appropriate Standard Species; 4.5.3.1 Biological Similarity; 4.5.3.2 Genome Size; 4.5.3.3 Nature of the Standard; 4.5.3.4 Availability; 4.5.3.5 Cytological Homogeneity; 4.5.3.6 Accessibility; 4.5.3.7 Reliability of C-Values; 4.5.4 Studies on Plant Standards; 4.5.5 Suggested Standards; 4.6 Fluorescence Inhibitors and Coatings of Debris; 4.6.1 What are Fluorescence Inhibitors and Coatings of Debris? 327 $a4.6.2 Experiments with Tannic Acid 330 $aTargeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies.With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hin 606 $aFlow cytometry 606 $aPlant cells and tissues 608 $aElectronic books. 615 0$aFlow cytometry. 615 0$aPlant cells and tissues. 676 $a571.62 701 $aDolez?el$b Jaroslav$0863264 701 $aGreilhuber$b Johann$0863265 701 $aSuda$b Jan$0863266 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910144559603321 996 $aFlow cytometry with plant cells$91926984 997 $aUNINA