LEADER 05437nam 2200637Ia 450 001 9910144559603321 005 20170814171740.0 010 $a1-280-92167-6 010 $a9786610921676 010 $a3-527-61092-8 010 $a3-527-61093-6 035 $a(CKB)1000000000377144 035 $a(EBL)481506 035 $a(OCoLC)173135610 035 $a(SSID)ssj0000156119 035 $a(PQKBManifestationID)11158482 035 $a(PQKBTitleCode)TC0000156119 035 $a(PQKBWorkID)10134196 035 $a(PQKB)10823756 035 $a(MiAaPQ)EBC481506 035 $a(EXLCZ)991000000000377144 100 $a20060529d2007 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aFlow cytometry with plant cells$b[electronic resource] $eanalysis of genes, chromosomes and genomes /$fedited by Jaroslav Dolez?el, Johann Greilhuber, and Jan Suda 210 $aWeinheim $cWiley-VCH ;$aChichester $cJohn Wiley [distributor]$d2007 215 $a1 online resource (481 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31487-3 320 $aIncludes bibliographical references and index. 327 $aFlow Cytometry with Plant Cells; Contents; Preface; List of Contributors; 1 Cytometry and Cytometers: Development and Growth; Overview; 1.1 Origins; 1.2 From Absorption to Fluorescence, from Imaging to Flow; 1.2.1 Early Microspectrophotometry and Image Cytometry; 1.2.2 Fluorescence Microscopy and the Fluorescent Antibody Technique; 1.2.3 Computers Meet Cytometers: The Birth of Analytical Flow Cytometry; 1.2.4 The Development of Cell Sorting; 1.3 The Growth of Multiparameter Flow Cytometry; 1.4 Bench-tops and Behemoths: Convergent Evolution; 1.5 Image Cytometry: New Beginnings?; References 327 $a2 Principles of Flow CytometryOverview; 2.1 Introduction; 2.2 A Brief History of Flow Cytometry; 2.3 Components of a Flow Cytometer; 2.3.1 Fluidics; 2.3.2 Optics; 2.3.3 Electronic Systems; 2.4 Flow Cytometric Informatics; 2.5 Spectral Compensation; 2.6 Cell Sorting; 2.7 Calibration Issues; 2.8 Conclusions; References; 3 Flow Cytometry with Plants: an Overview; Overview; 3.1 Introduction; 3.2 Fluorescence is a Fundamental Parameter; 3.3 Pushing Plants through the Flow Cytometer; 3.3.1 Difficulties with Plants and their Cells; 3.3.2 Protoplasts are somewhat ""Easier"" than Intact Cells 327 $a3.3.3 Going for Organelles3.4 Application of Flow Cytometry in Plants; 3.4.1 Microspores and Pollen; 3.4.2 Protoplasts; 3.4.2.1 Physiological Processes; 3.4.2.2 Secondary Metabolites; 3.4.2.3 Gene Expression; 3.4.2.4 Somatic Hybrids; 3.4.2.5 DNA Transfection; 3.4.3 Cell Nuclei; 3.4.3.1 Ploidy Levels; 3.4.3.2 Aneuploidy; 3.4.3.3 B Chromosomes; 3.4.3.4 Sex Chromosomes; 3.4.3.5 Cell Cycle and Endopolyploidy; 3.4.3.6 Reproductive Pathways; 3.4.3.7 Nuclear Genome Size; 3.4.3.8 DNA Base Content; 3.4.3.9 Chromatin Composition; 3.4.3.10 Sorting of Nuclei; 3.4.4 Mitotic Chromosomes; 3.4.5 Chloroplasts 327 $a3.4.6 Mitochondria3.4.7 Plant Pathogens; 3.4.8 Aquatic Flow Cytometry; 3.5 A Flow Cytometer in Every Laboratory?; 3.6 Conclusions and Future Trends; References; 4 Nuclear DNA Content Measurement; Overview; 4.1 Introduction; 4.2 Nuclear DNA Content: Words, Concepts and Symbols; 4.2.1 Replication-Division Phases; 4.2.2 Alternation of Nuclear Phases; 4.2.3 Generative Polyploidy Levels; 4.2.4 Somatic Polyploidy; 4.3 Units for Presenting DNA Amounts and their Conversion Factors; 4.4 Sample Preparation for Flow Cytometric DNA Measurement; 4.4.1 Selection of the Tissue; 4.4.2 Reagents and Solutions 327 $a4.4.2.1 Isolation Buffers and DNA Staining4.5 Standardization; 4.5.1 Types of Standardization; 4.5.2 Requirement of Internal Standardization - a Practical Test; 4.5.3 Choice of the Appropriate Standard Species; 4.5.3.1 Biological Similarity; 4.5.3.2 Genome Size; 4.5.3.3 Nature of the Standard; 4.5.3.4 Availability; 4.5.3.5 Cytological Homogeneity; 4.5.3.6 Accessibility; 4.5.3.7 Reliability of C-Values; 4.5.4 Studies on Plant Standards; 4.5.5 Suggested Standards; 4.6 Fluorescence Inhibitors and Coatings of Debris; 4.6.1 What are Fluorescence Inhibitors and Coatings of Debris? 327 $a4.6.2 Experiments with Tannic Acid 330 $aTargeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies.With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hin 606 $aFlow cytometry 606 $aPlant cells and tissues 608 $aElectronic books. 615 0$aFlow cytometry. 615 0$aPlant cells and tissues. 676 $a571.62 701 $aDolez?el$b Jaroslav$0863264 701 $aGreilhuber$b Johann$0863265 701 $aSuda$b Jan$0863266 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910144559603321 996 $aFlow cytometry with plant cells$91926984 997 $aUNINA LEADER 04619nam 2200613 450 001 9910787196603321 005 20170822144318.0 010 $a1-4704-1531-3 035 $a(CKB)3710000000230214 035 $a(EBL)3114224 035 $a(SSID)ssj0001108985 035 $a(PQKBManifestationID)11622326 035 $a(PQKBTitleCode)TC0001108985 035 $a(PQKBWorkID)11109317 035 $a(PQKB)10774882 035 $a(MiAaPQ)EBC3114224 035 $a(RPAM)17984926 035 $a(PPN)195408624 035 $a(EXLCZ)993710000000230214 100 $a20150417h20132013 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aCohomology for quantum groups via the geometry of the nullcone /$fChristopher P. Bendel [and three others] 210 1$aProvidence, Rhode Island :$cAmerican Mathematical Society,$d2013. 210 4$dİ2013 215 $a1 online resource (110 p.) 225 1 $aMemoirs of the American Mathematical Society,$x1947-6221 ;$vVolume 229, Number 1077 300 $a"Volume 229, Number 1077 (fourth of 5 numbers)." 311 $a0-8218-9175-8 320 $aIncludes bibliographical references. 327 $a""Contents""; ""Introduction""; ""Chapter 1. Preliminaries and Statement of Results""; ""1.1. Some preliminary notation""; ""1.2. Main results""; ""Chapter 2. Quantum Groups, Actions, and Cohomology""; ""2.1. Listings""; ""2.2. Quantum enveloping algebras""; ""2.3. Connections with algebraic groups""; ""2.4. Root vectors and PBW-basis""; ""2.5. Levi and parabolic subalgebras""; ""2.6. The subalgebra _{ }( _{ })""; ""2.7. Adjoint action""; ""2.8. Finite dimensionality of cohomology groups""; ""2.9. Spectral sequences and the Euler characteristic""; ""2.10. Induction functors"" 327 $a""Chapter 3. Computation of I??a??? and (I??a???)""""3.1. Subroot systems defined by weights""; ""3.2. The case of the classical Lie algebras""; ""3.3. The case of the exceptional Lie algebras""; ""3.4. Standardizing I??a???""; ""3.5. Resolution of singularities""; ""3.6. Normality of orbit closures""; ""Chapter 4. Combinatorics and the Steinberg Module""; ""4.1. Steinberg weights""; ""4.2. Weights of I??^{a???}_{ , }""; ""4.3. Multiplicity of the Steinberg module""; ""4.4. Proof of Proposition 4.2.1""; ""4.5. The weight _{ }""; ""4.6. Types _{ }, _{ }, _{ }""; ""4.7. Type _{ }"" 327 $a""4.8. Type _{ } with dividing +1""""4.9. Exceptional Lie algebras""; ""Chapter 5. The Cohomology Algebra ^{a???}( _{ }( ),a???)""; ""5.1. Spectral sequences, I""; ""5.2. Spectral sequences, II""; ""5.3. An identification theorem""; ""5.4. Spectral sequences, III""; ""5.5. Proof of main result, Theorem 1.2.3, I""; ""5.6. Spectral sequences, IV""; ""5.7. Proof of the main result, Theorem 1.2.3, II""; ""Chapter 6. Finite Generation""; ""6.1. A finite generation result""; ""6.2. Proof of part (a) of Theorem 1.2.4""; ""6.3. Proof of part (b) of Theorem 1.2.4"" 327 $a""Chapter 7. Comparison with Positive Characteristic""""7.1. The setting""; ""7.2. Assumptions""; ""7.3. Consequences""; ""7.4. Special cases""; ""Chapter 8. Support Varieties over _{ } for the Modules a???_{ }( ) and I??_{ }( )""; ""8.1. Quantum support varieties""; ""8.2. Lower bounds on the dimensions of support varieties""; ""8.3. Support varieties of a???_{ }( ): general results""; ""8.4. Support varieties of I??_{ }( ) when is good""; ""8.5. A question of naturality of support varieties""; ""8.6. The Constrictor Method I""; ""8.7. The Constrictor Method II"" 327 $a""8.8. Support varieties of a???_{ }( ) when is bad""""8.9. a??? when 3\mid ""; ""8.10. a??? when 3\mid ""; ""8.11. a??? when 3\mid ""; ""8.12. a??? when 3\mid , 5\mid ""; ""8.13. Support varieties of I??_{ }( ) when is bad""; ""Appendix A.""; ""A.1. Tables I""; ""A.2. Tables II""; ""Bibliography"" 410 0$aMemoirs of the American Mathematical Society ;$vVolume 229, Number 1077. 606 $aCohomology operations 606 $aAlgebraic topology 615 0$aCohomology operations. 615 0$aAlgebraic topology. 676 $a512/.55 700 $aBendel$b Christopher P.$f1969-$01583594 702 $aBendel$b Christopher P.$f1969- 712 02$aAmerican Mathematical Society. 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910787196603321 996 $aCohomology for quantum groups via the geometry of the nullcone$93866876 997 $aUNINA