LEADER 05158nam 2200625Ia 450 001 9910144558703321 005 20170810191434.0 010 $a1-280-72279-7 010 $a9786610722792 010 $a3-527-60801-X 010 $a3-527-60751-X 035 $a(CKB)1000000000377153 035 $a(EBL)481330 035 $a(OCoLC)78208847 035 $a(SSID)ssj0000218675 035 $a(PQKBManifestationID)11174992 035 $a(PQKBTitleCode)TC0000218675 035 $a(PQKBWorkID)10220266 035 $a(PQKB)11484630 035 $a(MiAaPQ)EBC481330 035 $a(PPN)137629222 035 $a(EXLCZ)991000000000377153 100 $a20050106d2006 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aPathogenomics$b[electronic resource] $egenome analysis of pathogenic microbes /$fedited by Jo?rg Hacker and Ulrich Dobrindt 210 $aWeinheim $cWiley-VCH ;$a[Chichester] $cWiley-VCH$dc2006 215 $a1 online resource (618 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31265-X 320 $aIncludes bibliographical references and index. 327 $aPathogenomics; Foreword; Contents; Preface; List of Contributors; Color Plates; I Methods; 1 Bioinformatics: Data Mining Among Genome Sequences; 1.1 Systematic Genome Analysis of Pathogens as a Basis for Pharmacogenomic Strategies; 1.2 Direct Sequence Annotation Tools for Functional Genomics; 1.3 Identification of Protein Function; 1.4 Obtaining Protein Information from a Domain Server; 1.5 Pathway Analysis; 1.6 Network Analysis; 1.7 Adaptation in Time and to Stimuli; 1.7.1 Experimental Design for Microarray Analysis; 1.7.2 Data Analysis; 1.8 Pathogen-Specific Challenges 327 $a1.9 Pathogen Adaptation Potential1.10 The Fight Against Resistance; 1.11 Drug Design and Antibiotics; 1.12 Annotation Platforms Suitable for Pathogenomics; 1.13 Conclusions; 2 Transcriptome Analysis: Towards a Comprehensive Understanding of Global Transcription Activity; 2.1 Introduction; 2.2 Development of Transcriptomics; 2.2.1 From Genomics to Functional Genomics; 2.2.2 From Gene to Whole Genome; 2.3 Introducing the Microarray; 2.3.1 What Is a Microarray?; 2.3.2 The Affymetrix Gene Chip; 2.3.3 The Spotted Microarray; 2.4 Microarray Methods; 2.4.1 Experimental Design 327 $a2.4.1.1 Type of Experiment2.4.1.2 Replicates; 2.4.2 RNA Extraction; 2.4.3 Labeling/Reverse Transcription; 2.4.4 Hybridization; 2.4.5 Scanning; 2.5 Data Normalization and Analysis; 2.5.1 Image Quantification; 2.5.2 Data Processing; 2.5.3 Data Analysis; 2.5.3.1 Detection of Differential Expression; 2.5.3.2 Pattern Recognition; 2.5.3.3 Graphical Representations; 2.5.6 Microarray Analysis Tools; 2.5.7 Microarray Follow-Up; 2.5.8 Data Storage and Reanalysis; 2.6 Transcriptomics: Where We Are Now and What's to Come 327 $a3 Physiological Proteomics of Bacillus subtilis and Staphylococcus aureus: Towards a Comprehensive Understanding of Cell Physiology and Pathogenicity3.1 Introduction; 3.2 Proteomics of Bacillus subtilis: The Gram-positive Model Organism; 3.2.1 The Vegetative Proteome; 3.2.2 Proteomes of Nongrowing Cells: Proteomic Signatures of Stress/Starvation Stimuli; 3.3 Physiological Proteomics of Staphylococcus aureus; 3.3.1 The Postgenome Era of S. aureus; 3.3.2 Proteomes of Growing and Nongrowing Cells; 3.3.3 Extracellular Proteins and Pathogenicity Networks 327 $a3.4 Outlook: Second Generation Proteomics and New Fields in S. aureus Physiology and Infection Biology4 Impact of Genome Sequences on Mutational Analysis of Fungal and Bacterial Pathogens; 4.1 The Long Road from Sequence to Function; 4.2 Classical Genetics Still at the Forefront in the Postgenome Era; 4.2.1 Reverse Genetics; 4.2.2 Transposon Mutagenesis; 4.3 Genome-Scale Mutational Analyses; 4.3.1 Saccharomyces cerevisiae; 4.3.2 Bacterial Workhorses: E. coli and Bacillus subtilis; 4.3.3 Bacterial Pathogens; 4.3.3.1 Mycoplasma Species; 4.3.3.2 Pseudomonas aeruginosa 327 $a4.3.3.3 Staphylococcus aureus 330 $aThe first book on this young, highly dynamic, and expanding field.This comprehensive, interdisciplinary text focuses on those pathogenic bacteria that are of high scientific and public health interest, yet which also display great potential for the development of new diagnostic, prophylactic and therapeutic procedures.The authors cover all aspects of pathogenomics, including methods, genomics and applications. In addition, the ongoing development of genome, transcriptome, proteome and bioinformatic analyses of pathogenic microorganisms and their host interactions makes for a comprehens 606 $aPathogenic microorganisms 606 $aMicrobiology 615 0$aPathogenic microorganisms. 615 0$aMicrobiology. 676 $a579.2 701 $aHacker$b Jo?rg$g(Jo?rg Hinrich)$0977277 701 $aDobrindt$b Ulrich$0977278 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910144558703321 996 $aPathogenomics$92226306 997 $aUNINA