LEADER 05398nam 2200673Ia 450 001 9910144323603321 005 20170810192820.0 010 $a1-280-85420-0 010 $a9786610854202 010 $a3-527-60754-4 010 $a3-527-60712-9 035 $a(CKB)1000000000376400 035 $a(EBL)481674 035 $a(OCoLC)70048393 035 $a(SSID)ssj0000175535 035 $a(PQKBManifestationID)11156097 035 $a(PQKBTitleCode)TC0000175535 035 $a(PQKBWorkID)10204132 035 $a(PQKB)11608884 035 $a(MiAaPQ)EBC481674 035 $a(EXLCZ)991000000000376400 100 $a20050519d2006 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aHydrolases in organic synthesis$b[electronic resource] $eregio- and stereoselective biotransformations /$fUwe T. Bornscheuer and Romas J. Kazlauskas 205 $a2nd ed. 210 $aWeinheim $cWiley-VCH ;$aChichester $cJohn Wiley$dc2006 215 $a1 online resource (369 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31029-0 320 $aIncludes bibliographical references and index. 327 $aHydrolases in Organic Synthesis; Preface for the 2(nd) edition; Preface for the 1(st) edition; Acknowledgments; Contents; 1 Introduction; 2 Designing Enantioselective Reactions; 2.1 Kinetic Resolutions; 2.1.1 Recycling and Sequential Kinetic Resolutions; 2.1.2 Dynamic Kinetic Resolutions; 2.1.2.1 Introduction; 2.1.2.2 Racemization by Protonation/Deprotonation; 2.1.2.3 Racemization by Addition/Elimination; 2.1.2.4 Racemization by Nucleophilic Substitution; 2.1.2.5 Racemization by Oxidation/Reduction; 2.1.2.6 Related Strategies; 2.2 Asymmetric Syntheses 327 $a3 Choosing Reaction Media: Water and Organic Solvents3.1 Hydrolysis in Water; 3.2 Transesterifications and Condensations in Organic Solvents; 3.2.1 Increasing the Catalytic Activity in Organic Solvents; 3.2.1.1 Choosing the Best Organic Solvent for High Activity; 3.2.2 Increasing the Enantioselectivity in Organic Solvents; 3.2.3 Water Content and Water Activity; 3.3 Other Reaction Media; 3.3.1 Ionic Liquids; 3.3.2 Reverse Micelles; 3.3.3 Supercritical Fluids; 3.4 Immobilization; 3.4.1 Introduction; 3.4.1.1 Increasing the Surface Area to Increase Catalytic Activity 327 $a4 Protein Sources and Optimization of Biocatalyst Performance4.1 Accessing Biodiversity; 4.2 Creating Improved Biocatalysts; 4.2.1 Directed Evolution; 4.2.1.1 Methods to Create Mutant Libraries; 4.2.1.2 Assay Systems; 4.2.1.3 Selected Examples; 4.2.2 Focused Directed Evolution; 4.3 Catalytic Promiscuity in Hydrolases; 4.3.1 Reactions Involving Functional Group Analogs; 4.3.1.1 Perhydrolases; 4.3.2 Aldol and Michael additions Catalyzed by Hydrolases; 4.3.2.1 Aldol Additions; 4.3.2.2 Michael-Type Additions; 4.3.3 Modifications to Introduce New Reactivity in Hydrolases 327 $a4.3.3.1 Enantioselective Reduction of Hydroperoxides with Selenosubtilisin4.3.3.2 Vanadate-Modified Phosphatases as Peroxidases; 5 Lipases and Esterases; 5.1 Availability, Structures and Properties; 5.1.1 Lipases; 5.1.1.1 Classification of Lipases; 5.1.1.2 General Features of PPL, PCL, CRL, CAL-B, and RML; 5.1.2 Esterases; 5.1.3 Lipases and Esterases are ?/? Hydrolases; 5.1.4 Lid or Flap in Interfacial Activation of Lipases; 5.1.5 Substrate Binding Site in Lipases and Esterases; 5.1.6 Designing Reactions with Lipases and Esterases; 5.1.6.1 Acyl Donor for Acylation Reactions 327 $a5.1.7 Assays for Lipases and Esterases5.1.7.1 Requirements for a Suitable Assay; 5.1.7.2 How to Distinguish Between Lipase, Esterase, and Protease; 5.2 Survey of Enantioselective Lipase-Catalyzed Reactions; 5.2.1 Alcohols; 5.2.1.1 Secondary Alcohols; 5.2.1.2 Primary Alcohols; 5.2.1.3 Other Alcohols, Amines, and Alcohol Analogs; 5.2.2 Carboxylic Acids; 5.2.2.1 General Considerations; 5.2.2.2 Carboxylic Acids with a Stereocenter at the ?-Position; 5.2.2.3 Carboxylic Acids with a Stereocenter at the ?-Position; 5.2.2.4 Other Carboxylic Acids; 5.2.2.5 Double Enantioselection; 5.2.2.6 Anhydrides 327 $a5.2.3 Lactones 330 $aFrom reviews to the first edtion:""Bornscheuer and Kazlauskas have set out, and succeeded, in producing a definitive manual on hydrolytic enzymes (especially lipases, esterases, and proteases) for organic chemists. This is quite simply the best book of its type and can be unreservedly recommended to organic chemists who have an interest in using hydrolytic enzymes in synthesis."" (Nicholas J. Turner, University of Edinburgh)""The book is an indispensable source of information on the use of hydrolases in organic synthesis. The subject matter is very well set out, and the chapter 606 $aCatalysis 606 $aHydrolases 606 $aOrganic compounds$xSynthesis 608 $aElectronic books. 615 0$aCatalysis. 615 0$aHydrolases. 615 0$aOrganic compounds$xSynthesis. 676 $a547.2 686 $a35.74$2bcl 700 $aBornscheuer$b U. T$g(Uwe Theo),$f1964-$0968009 701 $aKazlauskas$b R. J$g(Romas J.),$f1956-$0886693 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910144323603321 996 $aHydrolases in organic synthesis$92198463 997 $aUNINA