LEADER 05267nam 2200637 450 001 9910144278703321 005 20210104162133.0 010 $a1-281-31178-2 010 $a9786611311780 010 $a3-527-61709-4 010 $a3-527-61710-8 035 $a(CKB)1000000000376632 035 $a(EBL)481890 035 $a(OCoLC)609855476 035 $a(SSID)ssj0000295423 035 $a(PQKBManifestationID)11244958 035 $a(PQKBTitleCode)TC0000295423 035 $a(PQKBWorkID)10314762 035 $a(PQKB)10726953 035 $a(MiAaPQ)EBC481890 035 $a(EXLCZ)991000000000376632 100 $a20160820h20072007 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aEnzymes in industry $eproduction and applications /$fedited by Wolfgang Aehle 205 $aThird, completely revised edition. 210 1$aWeinheim, [Germany] :$cWiley-VCH Verlag GmbH & Co. KGaA,$d2007. 210 4$dİ2007 215 $a1 online resource (518 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31689-2 327 $aEnzymes in Industry; Contents; Preface to the Third Edition; List of Contributors; Abbreviations; 1 Introduction; 1.1 History; 1.2 Enzyme Nomenclature; 1.2.1 General Principles of Nomenclature; 1.2.2 Classification and Numbering of Enzymes; 1.3 Structure of Enzymes; 1.3.1 Primary Structure; 1.3.2 Three-Dimensional Structure; 1.3.3 Quaternary Structure, Folding, and Domains; 1.3.4 The Ribozyme; 1.4 Enzymes and Molecular Biology; 1.4.1 Biosynthesis of Enzymes; 1.4.2 Enzymes and DNA; 2 Catalytic Activity of Enzymes [57, 58, 60]; 2.1 Factors Governing Catalytic Activity [63]; 2.1.1 Temperature 327 $a2.1.2 Value of pH2.1.3 Activation; 2.1.4 Inhibition [65]; 2.1.5 Allostery [66]; 2.1.6 Biogenic Regulation of Activity; 2.2 Enzyme Assays; 2.2.1 Reaction Rate as a Measure of Catalytic Activity; 2.2.2 Definition of Units; 2.2.3 Absorption Photometry [71]; 2.2.4 Fluorometry [78]; 2.2.5 Luminometry [80]; 2.2.6 Radiometry; 2.2.7 Potentiometry [81]; 2.2.8 Conductometry; 2.2.9 Calorimetry; 2.2.10 Polarimetry; 2.2.11 Manometry; 2.2.12 Viscosimetry; 2.2.13 Turbidimetry; 2.2.14 Immobilized Enzymes [91]; 2.2.15 Electrophoresis; 2.3 Quality Evaluation of Enzyme Preparations; 2.3.1 Quality Criteria 327 $a2.3.2 Specific Activity2.3.3 Protein Determination; 2.3.4 Contaminating Activities; 2.3.5 Electrophoretic Purity; 2.3.6 High-Performance Liquid Chromatography [111]; 2.3.7 Performance Test; 2.3.8 Amino Acid Analysis and Protein Sequence Analysis; 2.3.9 Stability [93]; 2.3.10 Formulation of Enzyme Preparations; 3 General Production Methods; 3.1 Microbial Production; 3.1.1 Organism and Enzyme Synthesis; 3.1.2 Strain Improvement; 3.1.3 Physiological Optimization; 3.1.4 The Fermentor and its Limitations; 3.1.5 Process Design; 3.1.6 Modeling and Optimization; 3.1.7 Instrumentation and Control 327 $a3.2 Isolation and Purification [172-187]3.2.1 Preparation of Biological Starting Materials; 3.2.1.1 Cell Disruption by Mechanical Methods; 3.2.1.2 Cell Disruption by Nonmechanical Methods; 3.2.2. Separation of Solid Matter; 3.2.2.1 Filtration; 3.2.2.2 Centrifugation; 3.2.2.3 Extraction; 3.2.2.4 Flocculation and Flotation; 3.2.3 Concentration; 3.2.3.1 Thermal Methods; 3.2.3.2 Precipitation; 3.2.3.3 Ultrafiltration; 3.2.4 Purification; 3.2.4.1 Crystallization; 3.2.4.2 Electrophoresis; 3.2.4.3 Chromatography; 3.2.5 Product Formulation; 3.2.6 Waste Disposal; 3.3 Immobilization; 3.3.1 Definitions 327 $a3.3.2 History3.3.3 Methods; 3.3.3.1 Carrier Binding; 3.3.3.2 Cross-linking; 3.3.3.3 Entrapment; 3.3.4 Characterization; 3.3.5 Application; 4 Discovery and Development of Enzymes; 4.1 Enzyme Screening; 4.1.1 Overview; 4.1.2 Natural Isolate Screening; 4.1.3 Molecular Screening; 4.1.4 Environmental Gene Screening; 4.1.5 Genomic Screening; 4.1.6 Proteomic Screening; 4.2 Protein Engineering; 4.2.1 Introduction; 4.2.2 Application of Protein Engineering in Academia and Industry; 4.2.3 Outlook; 5 Industrial Enzymes; 5.1 Enzymes in Food Applications; 5.1.1 Enzymes in Baking; 5.1.1.1 Introduction 327 $a5.1.1.2 Amylases 330 $aLeading experts from all over the world present an overview of the use of enzymes in industry for:- the production of bulk products, such as glucose, or fructose- food processing and food analysis- laundry and automatic dishwashing detergents- the textile, pulp and paper and animal feed industries- clinical diagnosis and therapy- genetic engineering.The book also covers identification methods of new enzymes and the optimization of known ones, as well as the regulatory aspects for their use in industrial applications.Up to date and wide in scope, this 606 $aEnzymes$xBiotechnology 606 $aEnzymes$xIndustrial applications 608 $aElectronic books. 615 0$aEnzymes$xBiotechnology. 615 0$aEnzymes$xIndustrial applications. 676 $a660.6/34 676 $a660.634 702 $aAehle$b Wolfgang 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910144278703321 996 $aEnzymes in industry$92225597 997 $aUNINA