LEADER 05551nam 2200733Ia 450 001 9910144119703321 005 20200520144314.0 010 $a9786611840389 010 $a9781118681589 010 $a1118681584 010 $a9781281840387 010 $a1281840386 010 $a9780470516898 010 $a0470516895 010 $a9780470516881 010 $a0470516887 035 $a(CKB)1000000000549354 035 $a(EBL)366815 035 $a(OCoLC)437234418 035 $a(SSID)ssj0000182706 035 $a(PQKBManifestationID)11196813 035 $a(PQKBTitleCode)TC0000182706 035 $a(PQKBWorkID)10172658 035 $a(PQKB)10926525 035 $a(MiAaPQ)EBC366815 035 $a(PPN)188668187 035 $a(Perlego)1009364 035 $a(EXLCZ)991000000000549354 100 $a20070607d2007 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aIntroduction to mass spectrometry $einstrumentation, applications and strategies for data interpretation /$fJ. Throck Watson, O. David Sparkman 205 $a4th ed. 210 $aChichester, England ;$aHoboken, NJ $cJohn Wiley & Sons$dc2007 215 $a1 online resource (860 p.) 300 $aDescription based upon print version of record. 311 08$a9780470516348 311 08$a0470516348 320 $aIncludes bibliographic references and index. 327 $aIntroduction to Mass Spectrometry; Contents; Preface; Acknowledgments; Chapter 1 Introduction; I. Introduction; 1. The Tools and Data of Mass Spectrometry; 2. The Concept of Mass Spectrometry; II. History; III. Some Important Terminology Used In Mass Spectrometry; 1. Introduction; 2. Ions; 3. Peaks; 4. Resolution and Resolving Power; IV. Applications; 1. Example 1-1: Interpretation of Fragmentation Patterns (Mass Spectra) to Distinguish Positional Isomers; 2. Example 1-2: Drug Overdose: Use of GC/MS to Identify a Drug Metabolite 327 $a3. Example 1-3: Verification that the Proper Derivative of the Compound of Interest Has Been Prepared4. Example 1-4: Use of a CI Mass Spectrum to Complement an EI Mass Spectrum; 5. Example 1-5: Use of Exact Mass Measurements to Identify Analytes According to Elemental Composition; 6. Example 1-6: Is This Protein Phosphorylated? If So, Where?; 7. Example 1-7: Clinical Diagnostic Tests Based on Quantitation of Stable Isotopes by Mass Spectrometry in Lieu of Radioactivity; V. The Need for Chromatography; VI. Closing Remarks; VII. Monographs on Mass Spectrometry Published Before 1970 327 $aChapter 2 The Mass SpectrometerI. Introduction; II. Ion Guides; III. Types of m/z Analyzers; 1. Time-of-Flight m/z Analyzers; A. Linear; 1) Resolving Power of the Linear TOF Instrument; 2) Time-Lag Focusing; 3) Beam Deflection; B. Reflectron; C. Orthogonal Acceleration; D. Ion Detection in the TOF Analyzer; 1) Time-Slice Detection; 2) Time-Array Detection; 3) TAD with Transient Recorders; 4) TAD with an Integrating Transient Recorder; 5) Hadamard Transform TOF-MS; 2. Quadrupole Ion Traps; A. 3D Quadrupole Ion Trap; B. Linear Quadrupole Ion Trap (LIT); C. Performance Trade-Offs in the Ion Trap 327 $a3. The OrbitrapA. Historical Aspects; B. Operating Principles; 1) Role of the C Trap in Success of the Orbitrap; 2) Figures of Merit for the Orbitrap as an m/z Analyzer; 4. Transmission Quadrupoles; A. QMF Equations of Motion; B. The Stability Diagram; C. Characteristics of Output; D. Spectral Skewing; E. Performance Limitations; 5. Magnetic-Sector Instruments; A. Single-Focusing Instruments; 1) Operating Principles; 2) Magnetic Versus Scanning; 3) Performance Limitations; B. Double-Focusing Instruments; 6. FTICR-MS; A. Hardware Configuration; B. Operational Considerations 327 $aC. Representative Applications7. Ion Mobility Spectrometry (IMS); A. Operating Principles; B. FAIMS; C. Applications; IV. Calibration of the m/z Scale; 1. Electron Ionization; 2. Chemical Ionization; 3. Electrospray Ionization and APCI Techniques; 4. MALDI; V. Ion Detectors; 1. General Considerations; 2. Types of Detectors; A. Faraday Cup; B. Electron Multiplier; 1) Discrete-Dynode Version; 2) Continuous-Dynode Version; C. Negative-Ion Detection; D. Post-Acceleration Detection and Detection of High-Mass Ions; E. Channel Electron Multiplier Array (CEMA); F. Electro-Optical Ion Detection 327 $aG. The Daly Detector 330 $aCompletely revised and updated, this text provides an easy-to-read guide to the concept of mass spectrometry and demonstrates its potential and limitations. Written by internationally recognised experts and utilising ""real life"" examples of analyses and applications, the book presents real cases of qualitative and quantitative applications of mass spectrometry. Unlike other mass spectrometry texts, this comprehensive reference provides systematic descriptions of the various types of mass analysers and ionisation, along with corresponding strategies for interpretation of data. The book conclu 606 $aMass spectrometry 606 $aBiomolecules$xAnalysis 615 0$aMass spectrometry. 615 0$aBiomolecules$xAnalysis. 676 $a543 676 $a543.65 700 $aWatson$b J. Throck$095735 701 $aSparkman$b O. David$g(Orrin David),$f1942-$0447425 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910144119703321 996 $aIntroduction to mass spectrometry$9100774 997 $aUNINA