LEADER 02994nam 22005534a 450 001 9910144113903321 005 20230721033054.0 010 $a1-281-93903-X 010 $a9786611939038 010 $a0-470-42246-7 010 $a0-470-42245-9 035 $a(CKB)1000000000549814 035 $a(EBL)380571 035 $a(OCoLC)437241507 035 $a(SSID)ssj0000131475 035 $a(PQKBManifestationID)11134966 035 $a(PQKBTitleCode)TC0000131475 035 $a(PQKBWorkID)10026072 035 $a(PQKB)11497513 035 $a(MiAaPQ)EBC380571 035 $a(Au-PeEL)EBL380571 035 $a(CaPaEBR)ebr10263634 035 $a(CaONFJC)MIL193903 035 $a(EXLCZ)991000000000549814 100 $a20081021d2009 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aCoupled bioluminescent assays$b[electronic resource] $emethods, evaluations, and applications /$fMichael J. Corey 210 $aHoboken, N.J. $cWiley$dc2009 215 $a1 online resource (326 p.) 300 $aDescription based upon print version of record. 311 $a0-470-10883-5 320 $aIncludes bibliographical references (p. 265-289) and index. 327 $aIntroduction -- Coupled bioluminescent reactions in practice -- Coupled bioluminescent cytotoxicity assays -- The role of coupled bioluminescent assays in kinase screening and study -- Coupled bioluminescent phosphatase assays -- Acetylcholinesterase -- Measurement of nitric oxide synthase activity by coupled bioluminescence -- The coupled bioluminescent pyrophosphorolysis assay -- Coupled luminescent assays of G-protein-coupled receptors -- Coupled bioluminescent protease assays -- Coupled luminescent assays involving aequorin -- Coupled bioluminescent reporter assays -- Coupled bioluminescent assays : regulatory concerns -- Coupled bioluminescent determination of bioburden and sterility -- Environmental applications of coupled bioluminescent assays. 330 $aThis book highlights the applications of coupled bioluminescence assay techniques to real-world problems in drug discovery, environmental and chemical analysis, and biodefense. It separates theoretical aspects from the applied sections in a clear and readable way. Coupled Bioluminescent Assays, explains the uses of CB technologies across drug discovery to analyze toxicity, drug receptors, and enzymes. It covers applications in environmental analysis and biodefense, including cytotoxicity, fertilizer and explosives analysis, and nerve agent and pesticide detection. This is the premier reference 606 $aBioluminescence assay 615 0$aBioluminescence assay. 676 $a612/.01442 700 $aCorey$b Michael J$g(Michael James),$f1953-$0989967 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910144113903321 996 $aCoupled bioluminescent assays$92264268 997 $aUNINA