LEADER 01195nam0-2200385---450- 001 990008749710403321 005 20100226151000.0 010 $a9788829918249 035 $a000874971 035 $aFED01000874971 035 $a(Aleph)000874971FED01 035 $a000874971 100 $a20081107d2007----km-y0itay50------ba 101 1 $aita 102 $aIT 105 $aaf------001yy 200 1 $aBotanica sistematica$eun approccio filogenetico$fWalter S. Judd ... [et al.]$gedizione italiana a cura di M. Bigazzi ... [et al.] 205 $a2. ed.$frev. e agg. della 2. ed. ital. sulla 2. di lingua inglese a cura di Milena Rizzotto 210 $aPadova$cPiccin$dİ2007 215 $aXXI, 629 p.$cill.$d26 cm$e1 CD-rom 610 0 $aBotanica sistematica 610 0 $aFilogenesi 676 $a581.38 702 1$aJudd,$bWalter S. 702 1$aBigazzi,$bMassimo 702 1$aRizzotto,$bMilena 801 0$aIT$bUNINA$gRICA$2UNIMARC 901 $aBK 912 $a990008749710403321 952 $a581.38-JUD-1$b5511$fSC1 952 $a581.38-JUD-1A$b5512$fSC1 952 $a581.38-JUD-1B$b5498$fSC1 959 $aSC1 996 $aBotanica sistematica$9360155 997 $aUNINA LEADER 05453nam 2200661Ia 450 001 9910143983303321 005 20200327181921.0 010 $a1-280-72366-1 010 $a9786610723669 010 $a3-527-60932-6 010 $a3-527-60936-9 035 $a(CKB)1000000000376117 035 $a(EBL)481390 035 $a(OCoLC)159940039 035 $a(SSID)ssj0000171122 035 $a(PQKBManifestationID)11168132 035 $a(PQKBTitleCode)TC0000171122 035 $a(PQKBWorkID)10238020 035 $a(PQKB)10200160 035 $a(MiAaPQ)EBC481390 035 $a(EXLCZ)991000000000376117 100 $a20051013d2006 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aHigh-throughput screening in drug discovery$b[e-book] /$fedited by Jo?rg Hu?ser 210 $aWeinheim $cWiley-VCH ;$a[Chichester $cJohn Wiley, distributor]$dc2006 215 $a1 online resource (371 p.) 225 1 $aMethods and principles in medicinal chemistry ;$vv. 35 300 $aDescription based upon print version of record. 311 $a3-527-31283-8 320 $aIncludes bibliographical references and index. 327 $aHigh-Throughput Screening in Drug Discovery; Foreword; List of Contents; Preface; List of Contributors; Part I Concept of Screening; 1 Chemical Genetics: Use of High-throughput Screening to Identify Small-molecule Modulators of Proteins Involved in Cellular Pathways with the Aim of Uncovering Protein Function; 1.1 Introduction; 1.2 Classical and Chemical Genetics; 1.2.1 Forward and Reverse Screens; 1.3 Identifying Bioactive Molecules; 1.4 Target Identification; 1.4.1 Hypothesis-driven Target Identification; 1.4.2 Affinity-based Target Identification 327 $a1.4.3 Genomic Methods of Target Identification1.4.4 Proteomic Methods; 1.5 Discovery for Basic Research Versus Pharmacotherapy Goals; 1.6 Chemical Genetic Screens in the Academic Setting; 1.7 Conclusions; 2 High-throughput Screening for Targeted Lead Discovery; 2.1 Chemical Libraries for High-throughput Screening; 2.2 Properties of Lead Structures; 2.3 Challenges to High-throughput Screening; 2.4 Assay Technologies for High-throughput Screening; 2.5 Laboratory Automation; 2.6 From Target Selection to Confirmed Hits - the HTS Workflow and its Vocabulary 327 $a2.7 Separating Specific Modulators from Off-Target Effects2.8 Data Analysis and Screening Results; 2.9 Conclusions; Part II Automation Technologies; 3 Tools and Technologies that Facilitate Automated Screening; 3.1 Introduction - the Necessity to Automate; 3.1.1 Compound Libraries; 3.1.2 Targets and Data Points; 3.1.3 Main Issues Facing HTS Groups Today; 3.1.4 Benefits of Miniaturization; 3.1.5 Benefits of Automated HTS; 3.1.6 Screening Strategies; 3.1.7 Ultra HTS (UHTS); 3.2 Sample Carriers; 3.2.1 A Brief History of the Microplate; 3.2.2 Microplate Usage Today; 3.2.3 Microplate Arrays 327 $a3.2.4 Non-microplate Alternatives3.2.4.1 Labchips; 3.2.4.2 LabCDs; 3.2.4.3 LabBrick; 3.2.4.4 Arrayed Compound Screening; 3.3 Liquid Handling Tools; 3.3.1 Main Microplate Dispense Mechanisms; 3.3.1.1 Pin Tools; 3.3.1.2 Air and Positive Displacement; 3.3.1.3 Peristaltic; 3.3.1.4 Solenoid-syringe; 3.3.1.5 Solenoid-pressure bottle; 3.3.1.6 Capillary Sipper; 3.3.1.7 Piezoelectric; 3.3.1.8 Acoustic Transducer; 3.3.2 HTS Liquid Handling Applications and Dispensing Technologies Used; 3.3.2.1 Bulk Reagent and Cell Addition; 3.3.2.2 Compound Reformatting and Nanoliter Dispensing 327 $a3.3.2.3 Cherry Picking and Serial Dilution3.3.2.4 Microplate Washing; 3.4 Detection Technologies; 3.4.1 Main Detection Modalities Used in HTS; 3.4.2 Plate Readers; 3.4.3 Plate Imagers; 3.4.3.1 Macro-imaging; 3.4.3.2 Micro-imaging; 3.4.4 Dispense and Read Devices; 3.4.5 Other Detection Technologies; 3.4.6 Automation of Detection Technologies; 3.4.7 Potential Sources of Reading Error; 3.5 Laboratory Robotics; 3.5.1 Traditional Workstations; 3.5.2 Robotic Sample Processors; 3.5.3 Plate Storage Devices; 3.5.4 Plate Moving Devices; 3.5.5 Fully Integrated Robotic Systems; 3.5.6 Turnkey Workstations 327 $a3.5.7 Automated Cell Culture Systems 330 $aBacked by leading authorities, this is a professional guide to successful compound screening in pharmaceutical research and chemical biology, including the chemoinformatic tools needed for correct data evaluation. Chapter authors from leading pharmaceutical companies as well as from Harvard University discuss such factors as chemical genetics, binding, cell-based and biochemical assays, the efficient use of compound libraries and data mining using cell-based assay results.For both academics and professionals in the pharma and biotech industries working on small molecule screening. 410 0$aMethods and principles in medicinal chemistry ;$vv. 35. 606 $aHigh throughput screening (Drug development) 606 $aPharmaceutical chemistry 608 $aElectronic books. 615 0$aHigh throughput screening (Drug development) 615 0$aPharmaceutical chemistry. 676 $a615.19 676 $a615.1900285 686 $a44.38$2bcl 701 $aHu?ser$b Jo?rg$0958180 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910143983303321 996 $aHigh-throughput screening in drug discovery$92170849 997 $aUNINA