LEADER 05074nam 22006134a 450 001 9910143737903321 005 20180615155926.0 010 $a1-282-34592-3 010 $a9786612345920 010 $a0-470-05741-6 010 $a0-470-05740-8 035 $a(CKB)1000000000357282 035 $a(EBL)274319 035 $a(OCoLC)162105234 035 $a(SSID)ssj0000302912 035 $a(PQKBManifestationID)11235068 035 $a(PQKBTitleCode)TC0000302912 035 $a(PQKBWorkID)10274411 035 $a(PQKB)10564997 035 $a(MiAaPQ)EBC274319 035 $a(EXLCZ)991000000000357282 100 $a20060823d2007 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aMedical mycology$b[electronic resource] $ecellular and molecular techniques /$fedited by Kevin Kavanagh 210 $aChichester, England ;$aHoboken, NJ $cWiley$dc2007 215 $a1 online resource (350 p.) 300 $aDescription based upon print version of record. 311 $a0-470-01923-9 320 $aIncludes bibliographical references and index. 327 $aMedical Mycology; Contents; Preface; List of Contributors; 1 Diagnosis of Candida Infection in Tissue by Immunohistochemistry; 1.1 Introduction; 1.2 Specificity of monoclonal antibody 3H8 for C. albicans; Protocol 1.1 Testing of specificity of monoclonal antibody 3H8; 1.3 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms; Protocol 1.2 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms; 1.4 Application of immunohistochemistry in the diagnosis of Candida periodontal disease 327 $aProtocol 1.3 Use of monoclonal antibody 3H8 in the detection of C. albicans in tissue1.5 References; 2 Transmission Electron Microscopy of Pathogenic Fungi; 2.1 Introduction; 2.2 Glutaraldehyde-potassium-permanganate or glutaraldehyde-osmiumtetroxide fixation for ultrastructural analysis; Protocol 2.1 Glutaraldehyde-osmium tetroxide (#) or glutaraldehyde-potassium permanganate (*) fixation for ultrastructural analysis; 2.3 Identification of the different compartments of the secretory pathway in yeasts 327 $aProtocol 2.2 Identification of the different compartments of the secretory pathway in yeasts2.4 Cytochemical localization of acid phosphatase in yeasts; Protocol 2.3 Cytochemical localization of acid phosphatase in yeasts; 2.5 Detection of anionic sites; Protocol 2.4 Detection of anionic sites; 2.6 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg); Protocol 2.5 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg) 327 $a2.7 Enzyme-gold approach for the detection of polysaccharides in the cell wallProtocol 2.6 Enzyme-gold approach for the detection of polysaccharides in the cell wall; 2.8 Detection of glycoconjugates by the lectin-gold technique; Protocol 2.7 Detection of glycoconjugates by the lectin-gold technique; 2.9 Immunogold detection of antigens on ultrathin sections of acrylic resin; Protocol 2.8 Immunogold detection of antigens on ultrathin sections of acrylic resin; 2.10 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection 327 $aProtocol 2.9 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection2.11 Overview; 2.12 References; 3 Evaluation of Molecular Responses and Antifungal Activity of Phagocytes to Opportunistic Fungi; 3.1 Introduction; 3.2 Preparation of conidia and hyphae of opportunistic fungi; Protocol 3.1 Preparation of conidia and hyphae of opportunistic fungi; Protocol 3.2 Preparation of hyphal fragments; 3.3 Isolation of human monocytes from whole blood; Protocol 3.3 Isolation of human MNCs from whole blood; 3.4 Analysis of human MNC function in response to fungal infection 327 $aProtocol 3.4 XTT microassay 330 $aMedical Mycology: Cellular and Molecular techniques is a clear and concise overview of the subject that details the techniques essential for ongoing research in the area. Drawing together contributions from both scientists and clinicians working in the field, the text will provide a valuable perspective on the applicability of specific techniques to patient care. A wide range of molecular, immunological and cytological techniques are discussed throughout, with the inclusion of protocol section in each chapter designed to provide both a background a up-to-date account of the applicatio 606 $aMedical mycology$vLaboratory manuals 606 $aMycoses$xMolecular aspects$vLaboratory manuals 608 $aElectronic books. 615 0$aMedical mycology 615 0$aMycoses$xMolecular aspects 676 $a616.96901 701 $aKavanagh$b Kevin$0912684 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910143737903321 996 $aMedical mycology$92255266 997 $aUNINA