LEADER 05400nam 22006734a 450 001 9910143582603321 005 20170809162043.0 010 $a1-280-72166-9 010 $a9786610721665 010 $a0-470-08795-1 010 $a0-470-08794-3 035 $a(CKB)1000000000355408 035 $a(EBL)281847 035 $a(OCoLC)476027151 035 $a(SSID)ssj0000174659 035 $a(PQKBManifestationID)11171696 035 $a(PQKBTitleCode)TC0000174659 035 $a(PQKBWorkID)10174632 035 $a(PQKB)10516282 035 $a(MiAaPQ)EBC281847 035 $a(EXLCZ)991000000000355408 100 $a20060523d2007 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aHPLC for pharmaceutical scientists$b[electronic resource] /$fedited by Yuri Kazakevich, Rosario LoBrutto 210 $aHoboken, N.J. $cWiley-Interscience$dc2007 215 $a1 online resource (1136 p.) 300 $aDescription based upon print version of record. 311 $a0-471-68162-8 320 $aIncludes bibliographical references and indexes. 327 $aHPLC FOR PHARMACEUTICAL SCIENTISTS; CONTENTS; PREFACE; CONTRIBUTORS; PART I HPLC THEORY AND PRACTICE; 1 Introduction; 1.1 Chromatography in the Pharmaceutical World; 1.2 Chromatographic Process; 1.3 Classification; 1.4 History of Discovery and Early Development (1903-1933); 1.5 General Separation Process; 1.5.1 Modern HPLC Column; 1.5.2 HPLC System; 1.6 Types of HPLC; 1.6.1 Normal-Phase Chromatography (NP HPLC); 1.6.2 Reversed-Phase HPLC (RP HPLC or RPLC); 1.6.3 Ion-Exchange Chromatography (IEX); 1.6.4 Size-Exclusion Chromatography (SEC); 1.7 HPLC Descriptors (Vr, k, N, etc.) 327 $a1.7.1 Retention Volume1.7.2 Void Volume; 1.7.3 Retention Factor; 1.7.4 Selectivity; 1.7.5 Efficiency; 1.7.6 Resolution; References; 2 HPLC Theory; 2.1 Introduction; 2.2 Basic Chromatographic Descriptors; 2.3 Efficiency; 2.4 Resolution; 2.5 HPLC Retention; 2.6 Retention Mechanism; 2.7 General Column Mass Balance; 2.8 Partitioning Model; 2.9 Adsorption Model; 2.10 Total and Excess Adsorption; 2.11 Mass Balance in Adsorption Model; 2.12 Adsorption of the Eluent Components; 2.13 Void Volume Considerations; 2.14 Thermodynamic Relationships; 2.14.1 Effect of the Eluent Composition 327 $a2.15 Adsorption-Partitioning Retention Mechanism2.16 Secondary Equilibria; 2.16.1 Inclusion of Secondary Equilibria in the Mass Balance; 2.16.2 Salt Effect; 2.17 Gradient Elution Principles; 2.18 Types of Analyte Interactions with the Stationary Phase; 2.19 Conclusion; References; 3 Stationary Phases; 3.1 Introduction; 3.2 Type of Packing Material (Porous, Nonporous, Monolithic); 3.3 Base Material (Silica, Zirconia, Alumina, Polymers); 3.4 Geometry; 3.4.1 Shape (Spherical/Irregular); 3.4.2 Particle Size Distribution; 3.4.3 Surface Area; 3.4.4 Pore Volume; 3.4.5 Surface Geometry 327 $a3.5 Adsorbent Surface Chemistry3.5.1 Surface Chemistry of the Base Material; 3.5.2 Silica; 3.5.3 Silica Hybrid; 3.5.4 Polymeric Packings; 3.5.5 Zirconia (Metal Oxides); 3.5.6 Porous Carbon (or Carbon-Coated Phases); 3.6 Surface of Chemically Modified Material; 3.6.1 Limits of Surface Modification; 3.6.2 Chemical Modification; 3.6.3 Types of Bonded Phases; 3.6.4 Structure of the Bonded Layer; 3.6.5 Density of Bonded Ligands; 3.6.6 Residual Silanoles; 3.6.7 Surface Area of Modified Adsorbent; 3.7 Polymer-Based Adsorbents; 3.8 Stationary Phases for Chiral Separations 327 $a3.8.1 Polysaccharide-Coated Phases3.8.2 Pirkle-Type Phases; 3.8.3 Protein Phases; 3.8.4 Molecular Imprinted Polymers for Chiral Separations; 3.9 Columns; 3.9.1 Capillary/Monolithic/Packed Columns; 3.9.2 Column Cleaning; 3.9.3 Column Void Volume; 3.9.4 Mass of Adsorbent in the Column; References; 4 Reversed-Phase HPLC; 4.1 Introduction; 4.2 Retention in Reversed-Phase HPLC; 4.3 Stationary Phases for RPLC; 4.4 Mobile Phases for RPLC; 4.4.1 Eluent Composition and Solvent Strength of the Mobile Phase; 4.4.2 Type of Organic Modifier 327 $a4.4.3 Selectivity as a Function of Type and Concentration of Organic Composition 330 $aHPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry. In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. 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