LEADER 05316nam 22006494a 450 001 9910143569103321 005 20201217184757.0 010 $a1-280-27734-3 010 $a9786610277346 010 $a0-471-73658-9 010 $a0-471-73657-0 035 $a(CKB)1000000000355515 035 $a(EBL)233617 035 $a(OCoLC)171257493 035 $a(SSID)ssj0000190027 035 $a(PQKBManifestationID)11165843 035 $a(PQKBTitleCode)TC0000190027 035 $a(PQKBWorkID)10187510 035 $a(PQKB)11720883 035 $a(MiAaPQ)EBC233617 035 $a(PPN)150720467 035 $a(EXLCZ)991000000000355515 100 $a20041229d2005 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aLC/MS $ea practical user's guide /$fMarvin C. McMaster 210 $aHoboken, N.J. $cJohn Wiley$dc2005 215 $a1 online resource (179 p.) 300 $aDescription based upon print version of record. 311 $a0-471-65531-7 320 $aIncludes bibliographical references (p. 155-156) and index. 327 $aLC/MS; CONTENTS; Preface; 1 Introduction to LC/MS; 1.1 Why LC/MS?; 1.2 Molecular Weights and Structure Studies; 1.3 LC/MS Systems; 1.4 System Costs; 1.5 Competitive Systems; 2 The HPLC System; 2.1 HPLC System Components; 2.2 Gradient versus Isocratic Systems; 2.3 Micro HPLC Systems; 2.4 HPLC Tubing and Fittings; 3 The HPLC Column and Separation Modes; 3.1 Column Construction; 3.2 Column Packing Materials; 3.3 Normal-Phase Columns; 3.4 Other Bonded-Phase Silica Columns; 3.5 Optimizing Reverse-Phase Column Use; 3.6 Silica Ion-Exchange Columns; 3.7 Silica Size-Separation Columns 327 $a3.8 Zirconium Bonded-Phase Columns3.9 Polymer Reverse-Phase Columns; 4 HPLC and Column Maintenance; 4.1 HPLC Maintenance; 4.2 Column Maintenance; 5 Sample Preparation and Separations Development; 5.1 Mobile-Phase Preparation; 5.2 Mobile-Phase pH Control Using Buffers; 5.3 Sample Preparation; 5.4 Cartridge Column Cleanup; 5.5 On-Column Sample Concentration; 5.6 Isocratic and Gradient Methods Development; 5.7 Automated Methods Development; 6 LC/MS Interfaces; 6.1 Solvent Removal and Ionization; 6.2 Atmospheric-Pressure Interfaces; 6.3 Electrospray Interface; 6.4 Ion Spray Interface 327 $a6.5 Secondary Detectors7 LC/MS Overview; 7.1 HPLC and the Ionization Source; 7.2 Vacuum Pumps; 7.3 Analyzer and Ion Detector Designs; 7.4 Data and Control Systems; 7.5 Peak Detection, ID, and Quantitation; 8 Mass Analyzers; 8.1 Quadrupole Analyzer; 8.2 Ion Trap Analyzer; 8.3 Linear Ion Trap Analyzer; 8.4 Time-of-Flight Analyzer; 8.5 Fourier Transform Analyzer Design; 8.6 Magnetic Sector Analyzers; 9 Mass Spectrometer Maintenance; 9.1 High-Vacuum Operation; 9.2 MS Hardware Maintenance; 9.3 System Electrical Grounding; 10 Application Areas for LC/MS; 10.1 Compound Discovery 327 $a10.2 Identification of Complex Biological Compounds10.3 Analysis of Trace Impurities and Metabolites; 10.4 Arson Residue Investigation; 10.5 Industrial Water and Pesticide Analysis; 10.6 Toxicology and Drugs of Abuse; 10.7 Clinical Therapeutic Drug Screening; 10.8 Pesticide Manufacturing; 11 Trace Analysis and LC/MS/MS; 11.1 LC/MS/MS Triple-Quadrupole System; 11.2 MS/MS Operating Modes; 11.3 Ion Trap MS/MS Operation; 11.4 Hybrid LC/MS/MS Systems; 12 Drug Discovery and Benchtop LC/MS; 12.1 Activity Screening; 12.2 Standardized LC/MS Screening 327 $a12.3 Molecular Fragmentation for Structural Determination12.4 Process Monitoring; 13 Proteomics: LC/MALDI/TOF and MS/MS Libraries; 13.1 Protein Molecular-Weight Determination by LC/MS; 13.2 De Novo Protein Purification; 13.3 Protein Analysis by Two-Dimensional GEP and LC/TOFMS; 13.4 LC/MS/MS Identification of Peptide Structures; 13.5 Tracer Labeling for Peptide ID; 13.6 Posttranslational Modified Protein; 13.7 Transient Peptides and Accumulation Proteins; 14 The Future of LC/MS; 14.1 Instrumentation Improvements; 14.2 Affordable Benchtop LC/LITMS; 14.3 User-Customized Data Libraries 327 $a14.4 Nucleomics and Restriction Fragment Analysis 330 $aA practical guide to using and maintaining an LC/MS systemThe combination of liquid chromatography (LC) and mass spectrometry (MS) has become the laboratory tool of choice for a broad range of industries that require the separation, analysis, and purification of mixtures of organic compounds.LC/MS: A Practical User's Guide provides LC/MS users with an easy-to-use, hands-on reference that focuses on the practical applications of LC/MS and introduces the equipment and techniques needed to use LC/MS successfully. Following a thorough explanation of the basic components and 606 $aLiquid chromatography$vHandbooks, manuals, etc 606 $aHigh performance liquid chromatography$vHandbooks, manuals, etc 606 $aMass spectrometry$vHandbooks, manuals, etc 615 0$aLiquid chromatography 615 0$aHigh performance liquid chromatography 615 0$aMass spectrometry 676 $a543.84 676 $a543/.84 700 $aMcMaster$b Marvin C$091640 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910143569103321 996 $aLC$91996062 997 $aUNINA