LEADER 08946nam 22014293u 450 001 9910143510103321 005 20221021191535.0 010 $a0-470-85690-4 010 $a1-282-34827-2 010 $a0-470-51870-7 010 $a0-470-01734-1 010 $a9786612348273 010 $a1-280-62202-4 010 $a9786610622023 010 $a0-470-85691-2 035 $a(CKB)111087027141122 035 $a(EBL)470502 035 $a(OCoLC)298129955 035 $a(SSID)ssj0000296942 035 $a(PQKBManifestationID)11227030 035 $a(PQKBTitleCode)TC0000296942 035 $a(PQKBWorkID)10332397 035 $a(PQKB)10286724 035 $a(SSID)ssj0000158763 035 $a(PQKBManifestationID)11164060 035 $a(PQKBTitleCode)TC0000158763 035 $a(PQKBWorkID)10150021 035 $a(PQKB)11065504 035 $a(MiAaPQ)EBC470502 035 $a(MiAaPQ)EBC4956747 035 $a(Au-PeEL)EBL4956747 035 $a(CaONFJC)MIL62202 035 $a(OCoLC)1027141645 035 $a(EXLCZ)99111087027141122 100 $a20151012d2008|||| u|| | 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aFrom genes to genomes$b[electronic resource] $econcepts and applications of DNA technology 205 $a2nd ed.$bfirst published 2002. 210 $aHoboken $cWiley$d2008 215 $a1 online resource (396 p.) 300 $aDescription based upon print version of record. 311 $a0-471-49783-5 311 $a0-471-49782-7 327 $a""From Genes to Genomes""; ""Contents""; ""Preface""; ""1: Introduction""; ""2: Basic Molecular Biology""; ""2.1 Nucleic acid structure""; ""2.2 What is a gene?""; ""2.3 Information flow: gene expression""; ""2.4 Gene structure and organization""; ""3: How to Clone a Gene""; ""3.1 What is cloning?""; ""3.2 Overview of the procedures""; ""3.3 Gene libraries""; ""3.4 Hybridization""; ""3.5 Polymerase chain reaction""; ""3.6 Extraction and purification of nucleic acids""; ""3.7 Detection and quantitation of nucleic acids""; ""3.8 Gel electrophoresis""; ""4: Cutting and Joining DNA"" 327 $a""4.1 Restriction endonucleases""""4.2 Ligation""; ""4.3 Modification of restriction fragment ends""; ""4.4 Other ways of joining DNA molecules""; ""5: Vectors""; ""5.1 Plasmid vectors""; ""5.2 Vectors based on the lambda bacteriophage""; ""5.3 Cosmids""; ""5.4 M13 vectors""; ""5.5 Expression vectors""; ""5.6 Vectors for cloning and expression in eukaryotic cells""; ""5.7 Supervectors: YACs and BACs""; ""5.8 Summary""; ""6: Genomic and cDNA Libraries""; ""6.1 Genomic libraries""; ""6.2 Growing and storing libraries""; ""6.3 cDNA libraries""; ""6.4 Random, arrayed and ordered libraries"" 327 $a""7: Finding the Right Clone""""7.1 Screening libraries with gene probes""; ""7.2 Screening expression libraries with antibodies""; ""7.3 Subcloning""; ""7.4 Characterization of plasmid clones""; ""8: Polymerase Chain Reaction""; ""8.1 The PCR reaction""; ""8.2 PCR in practice""; ""8.3 Cloning PCR products""; ""8.4 Long-range PCR""; ""8.5 Reverse-transcription PCR""; ""8.6 Rapid amplification of cDNA ends""; ""8.7 Quantitative PCR""; ""8.8 Applications of PCR""; ""9: Characterization of a Cloned Gene""; ""9.1 DNA sequencing""; ""9.2 Databank entries and annotation""; ""9.3 Sequence analysis"" 327 $a""9.4 Sequence comparisons""""9.5 Protein structure""; ""9.6 Confirming gene function""; ""10: Analysis of Gene Expression""; ""10.1 Analysing transcription""; ""10.2 Methods for studying the promoter""; ""10.3 Regulatory elements and DNA-binding proteins""; ""10.4 Translational analysis""; ""11: Products from Native and Manipulated Cloned Genes""; ""11.1 Factors affecting expression of cloned genes""; ""11.2 Expression of cloned genes in bacteria""; ""11.3 Expression in eukaryotic host cells""; ""11.4 Adding tags and signals""; ""11.5 In vitro mutagenesis""; ""11.6 Vaccines"" 327 $a""12: Genomic Analysis""""12.1 Genome sequencing""; ""12.2 Analysis and annotation""; ""12.3 Comparing genomes""; ""12.4 Genome browsers""; ""12.5 Relating genes and functions: genetic and physical maps""; ""12.6 Transposon mutagenesis and other screening techniques""; ""12.7 Conclusion""; ""13: Analysis of Genetic Variation""; ""13.1 Single nucleotide polymorphisms""; ""13.2 Larger-scale variations""; ""13.3 Other methods for studying variation""; ""13.4 Human genetic diseases""; ""13.5 Molecular phylogeny""; ""14: Post-genomic Analysis""; ""14.1 Analysing transcription; transcriptomes"" 327 $a""14.2 Array-based methods"" 330 $aRapid advances in our understanding of genetics have required that new books contain topics such as the concept and theory of gene cloning, transgenics, genomics, and various other coverage of traditional and contemporary subjects. Although there is an abundance of textbooks that cover introductory genetics and advanced courses in genetics, there is a noticeable gap at the intermediate (second year) level. In the past gene structure, function and expression were taught at final year /postgraduate level, but the rapid advances in our understanding of genetics has encouraged courses to change considerably. Over recent years these topics have filtered down the curriculum and are currently taught as core topics at second year, with a corresponding change in textbook requirements. Where once second year students were restricted to learning about the concept and theory of gene cloning, now they routinely clone genes for themselves as part of their practical assignments. Genes to Genomics will fill the gap, cover much of the same ground as previous titles, but go further on contemporary topics like transgenics, sequence comparison and analysis of variation.; A concise, up to date textbook that provides a balanced coverage of traditional and contemporary topics taught within intermediate courses in molecular genetics Jeremy Dale has a proven track record as the successful author of Molecular Genetics of Bacteria Genes to Genomics will include a series of feature box-outs that will examine some of the topical issues related to the scientific concepts and examples explored within the text A range of questions and exercises including worked examples and web-based practicals An accompanying web site will allow the authors to keep their audience up to date in the areas that are prone to date most rapidly between successive editions of the textbook. It will 330 8 $aalso include the illustrations and images from the textbook, in addition to worked examples, answers to questions within the book, and links to related websites of key interest. 606 $aCloning, Molecular 606 $aDNA, Recombinant 606 $aDNA 606 $aGenes 606 $aGenetic Engineering 606 $aGenomes 606 $aGenetic engineering 606 $aDNA 606 $aGenes 606 $aGenomes 606 $aGenetic Engineering 606 $aDNA, Recombinant 606 $aCloning, Molecular 606 $aGenome 606 $aGenetic Structures 606 $aGenetic Techniques 606 $aDNA 606 $aInvestigative Techniques 606 $aGenetic Phenomena 606 $aNucleic Acids 606 $aAnalytical, Diagnostic and Therapeutic Techniques and Equipment 606 $aPhenomena and Processes 606 $aNucleic Acids, Nucleotides, and Nucleosides 606 $aChemicals and Drugs 606 $aGenetics$2HILCC 606 $aBiology$2HILCC 606 $aHealth & Biological Sciences$2HILCC 608 $aElectronic books. 615 4$aCloning, Molecular. 615 4$aDNA, Recombinant. 615 4$aDNA. 615 4$aGenes. 615 4$aGenetic Engineering. 615 4$aGenomes. 615 0$aGenetic engineering 615 0$aDNA 615 0$aGenes 615 0$aGenomes 615 2$aGenetic Engineering 615 2$aDNA, Recombinant 615 2$aCloning, Molecular 615 2$aGenome 615 2$aGenetic Structures 615 2$aGenetic Techniques 615 2$aDNA 615 2$aInvestigative Techniques 615 2$aGenetic Phenomena 615 2$aNucleic Acids 615 2$aAnalytical, Diagnostic and Therapeutic Techniques and Equipment 615 2$aPhenomena and Processes 615 2$aNucleic Acids, Nucleotides, and Nucleosides 615 2$aChemicals and Drugs 615 7$aGenetics 615 7$aBiology 615 7$aHealth & Biological Sciences 676 $a572.8 676 $a572.86 676 $a660.65 700 $aDale$b Jeremy$g(Jeremy W.)$0324172 701 $avon Schantz$b Malcolm$0927364 801 0$bAU-PeEL 801 1$bAU-PeEL 801 2$bAU-PeEL 906 $aBOOK 912 $a9910143510103321 996 $aFrom Genes to Genomes$92083589 997 $aUNINA