LEADER 05484nam 2200709Ia 450 001 9910143509103321 005 20190618170550.0 010 $a1-280-36704-0 010 $a9786610367047 010 $a0-470-32291-8 010 $a0-471-46186-5 010 $a0-471-22056-6 035 $a(CKB)111087027143190 035 $a(EBL)469088 035 $a(OCoLC)53228681 035 $a(SSID)ssj0000161756 035 $a(PQKBManifestationID)11181661 035 $a(PQKBTitleCode)TC0000161756 035 $a(PQKBWorkID)10198596 035 $a(PQKB)11137308 035 $a(MiAaPQ)EBC469088 035 $a(EXLCZ)99111087027143190 100 $a19980805d1999 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aGenomics$b[electronic resource] $ethe science and technology behind the human genome project /$fCharles R. Cantor, Cassandra L. Smith 210 $aNew York $cJohn Wiley & Sons$dc1999 215 $a1 online resource (621 p.) 225 1 $aBaker Lecture Series ;$vv.12 300 $a"A Wiley-Interscience publication." 311 $a0-471-59908-5 320 $aIncludes bibliographies and index. 327 $aContents; Preface; Introduction; 1 DNA Chemistry and Biology; Basic Properties of DNA; Covalent Structure; Double Helical Structure; Methylated Bases; Plasticity in DNA Structure; DNA Synthesis; DNA as a Flexible Set of Chemical Reagents; Basic DNA Biology; Genome Sizes; Number of Genes; Sources and Additional Readings; 2 A Genome Overview at the Level of Chromosomes; Basic Properties of Chromosomes; Bacterial Chromosomes; Chromosomes of Eukaryotic Organisms; Centromeres; Telomeres; Dynamic Behavior of Telomeres; Chromatin and the Higher-Order Structure of Chromosomes 327 $aChromosomes in the Cell CycleGenome Organization; Chromosome Purification; Chromosome Number; Unusual Characteristics of Sex Chromosomes and Mitochondria; Synteny; Sources and Additional Readings; 3 Analysis of DNA Sequences by Hybridization; Basic Requirements for Selectivity and Sensitivity; Detection of Specific DNA Sequences; Equilibria between DNA Double and Single Strands; Thermodynamics of the Melting of Short Duplexes; Thermodynamics of Imperfectly Paired Duplexes; Kinetics of the Melting of Short Duplexes; Kinetics of Melting of Long DNA; Kinetics of Double-Strand Formation 327 $aComplexityHybridization on Filters; Sensitive Detection; Sources and Additional Readings; 4 Polymerase Chain Reaction and Other Methods for In Vitro DNA Amplification; Why Amplify DNA?; Basic Principles of the Polymerase Chain Reaction (PCR); Noise in PCR: Contamination; PCR Noise: Mispriming; Misincorporation; Long PCR; Incorporating Extra Functionalities; Single-Sided PCR; Reducing Complexity with PCR; Additional Variants of the Basic PCR Reaction; Total Genome Amplification Methods; Application of PCR to Detect Molecules Other Than DNA 327 $aDNA Amplification without Thermal Cycling and Other Alternatives to PCRFuture of PCR; Sources and Additional Readings; 5 Principles of DNA Electrophoresis; Physical Fractionation of DNA; Separation of DNA in the Ultracentrifuge; Electrophoretic Size Separations of DNA; Electrophoresis without Gels; Motions of DNA Molecules in Gels; Complex Effects of Gel Structure and Behavior; Biased Reptation Model of DNA Behavior in Gels; Pulsed Field Gel Electrophoresis (PFG); Macroscopic Behavior of DNA in PFG; Inadequacy of Reptation Models for PFG; DNA Trapping Electrophoresis 327 $aSecondary Pulsed Field Gel Electrophoresis (SPFG)Entry of DNAs into Gels; Sources and Additional Readings; 6 Genetic Analysis; Why We Need Genetics; Basic Strategy for Genetic Analysis in the Human: Linkage Mapping; A Glossary of Genetic Terms; Relationship between the Physical and the Genetic Maps; Power of Mouse Genetics; Weakness of Human Genetics; Linkage Analysis Ignoring Recombination; Linkage Analysis with Recombination; Interval Mapping; Finding Genes by Genetic Mapping; Moving from Weak Linkage Closer to a Gene; Linkage Disequilibrium 327 $aComplications in Linkage Disequilibrium and Genetic Maps in General 330 $aA unique exploration of the principles and methods underlying the Human Genome Project and modern molecular genetics and biotechnology-from two top researchers In Genomics, Charles R. Cantor, former director of the Human Genome Project, and Cassandra L. Smith give the first integral overview of the strategies and technologies behind the Human Genome Project and the field of molecular genetics and biotechnology. Written with a range of readers in mind-from chemists and biologists to computer scientists and engineers-the book begins with a review of the basic properties of DNA and the chromoso 410 0$aGeorge Fisher Baker non-resident lectureship in chemistry at Cornell University ;$vv. 12 606 $aDNA$xAnalysis 606 $aNucleotide sequence 606 $aGene mapping 608 $aElectronic books. 615 0$aDNA$xAnalysis. 615 0$aNucleotide sequence. 615 0$aGene mapping. 676 $a572.8/6 676 $a572.86 700 $aCantor$b Charles R.$f1942-$045546 701 $aSmith$b Cassandra$0857175 712 02$aHuman Genome Project. 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910143509103321 996 $aGenomics$91913997 997 $aUNINA