LEADER 04214nam 22006134a 450 001 9910143407703321 005 20170815122206.0 010 $a1-280-28688-1 010 $a9786610286881 010 $a0-470-32559-3 010 $a0-471-73949-9 010 $a0-471-73948-0 035 $a(CKB)1000000000355062 035 $a(EBL)242871 035 $a(OCoLC)85784717 035 $a(SSID)ssj0000165610 035 $a(PQKBManifestationID)11180866 035 $a(PQKBTitleCode)TC0000165610 035 $a(PQKBWorkID)10162668 035 $a(PQKB)10885562 035 $a(MiAaPQ)EBC242871 035 $a(EXLCZ)991000000000355062 100 $a20041213d2006 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aGreen fluorescent protein$b[electronic resource] $eproperties, applications, and protocols /$fedited by Martin Chalfie, Steven R. Kain 205 $a2nd ed. 210 $aHoboken, N.J. $cWiley-Interscience$dc2006 215 $a1 online resource (486 p.) 225 1 $aMethods of biochemical analysis ;$vv. 47 300 $aDescription based upon print version of record. 311 $a0-471-73682-1 320 $aIncludes bibliographical references and index. 327 $aDiscovery of green fluorescent protein / Osamu Shimomura -- Photons for reporting molecular events: green fluorescent protein and four luciferase systems / J. Woodland Hastings and James G. Morin -- Biochemical and physical properties of green fluorescent protein / William W. Ward -- The three-dimensional structure of green fluorescent protein and its implications for function and design / George N. Phillips, Jr -- Molecular biology and mutation of green fluorescent protein / David A. Zacharias, Roger Y. Tsien -- Discovery and properties of GFP-like proteins from nonbioluminescent anthozoa / Konstantin A. Lukyanov ... [et al.] -- Evolution of function and color in GFP-like proteins / Mikhail V. Matz, Yulii A. Labas, and Juan Ugalde -- The uses of green fluorescent protein in prokaryotes / Raphael H. Valdivia, Brendan P. Cormack, and Stanley Falkow -- The uses of green fluorescent protein in yeasts / Amy L. Hitchcock, Jason A. Kahana, and Pamela A. Silver -- Uses of GFP in Caenorhabditis elegans / Oliver Hobert and Paula Loria -- Green fluorescent protein applications in drosophila / Tulle Hazelrigg and Jennifer H. Mansfield -- The uses of green fluorescent protein in plants / Jim Haseloff and Kirby R. Siemering -- Uses of GFP in transgenic vertebrates / Sean Magason ... [et al.] -- The uses of green fluorescent protein in mammalian cells / Theresa H. Ward and Jennifer Lippincott-Schwartz -- Practical considerations for use of reef coral fluorescent proteins in mammalian cells: applications in fluorescent microscopy and flow cytometry / Yu Fang ... [et al.] -- Pharmaceutical applications of GFP and RCFP / Nicola Bevan and Stephen Rees -- Reassembled GFP: detecting protein-protein interactions and protein expression patterns / Thomas J. Magliery and Lynne Regan. 330 $aSince the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biolog 410 0$aMethods of biochemical analysis ;$vv. 47. 606 $aGreen fluorescent protein$vLaboratory manuals 608 $aElectronic books. 615 0$aGreen fluorescent protein 676 $a543.8 676 $a572.6 676 $a572/.6 701 $aChalfie$b Martin$0981313 701 $aKain$b Steven$0981314 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910143407703321 996 $aGreen fluorescent protein$92239850 997 $aUNINA