LEADER 05331nam 22006734a 450 001 9910143397603321 005 20210216173623.0 010 $a1-119-29360-X 010 $a1-280-45047-9 010 $a9786610450473 010 $a0-470-24575-1 010 $a0-471-97310-6 010 $a0-471-97309-2 035 $a(CKB)1000000000355118 035 $a(EBL)258897 035 $a(OCoLC)166291110 035 $a(SSID)ssj0000204369 035 $a(PQKBManifestationID)11199472 035 $a(PQKBTitleCode)TC0000204369 035 $a(PQKBWorkID)10188666 035 $a(PQKB)10404616 035 $a(MiAaPQ)EBC258897 035 $a(MiAaPQ)EBC4499677 035 $a(PPN)152469281 035 $a(EXLCZ)991000000000355118 100 $a20051104d2006 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aModern HPLC for practicing scientists /$fby Michael Dong 210 $aHoboken, N.J. $cWiley-Interscience$d2006 215 $a1 online resource (306 p.) 300 $aDescription based upon print version of record. 311 $a0-471-72789-X 320 $aIncludes bibliographical references and index. 327 $aMODERN HPLC FOR PRACTICING SCIENTISTS; CONTENTS; Preface; 1 Introduction; 1.1 Introduction; 1.1.1 Scope; 1.1.2 What Is HPLC?; 1.1.3 A Brief History; 1.1.4 Advantages and Limitations; 1.2 Modes of HPLC; 1.2.1 Normal-Phase Chromatography (NPC); 1.2.2 Reversed-Phase Chromatography (RPC); 1.2.3 Ion-Exchange Chromatography (IEC); 1.2.4 Size-Exclusion Chromatography (SEC); 1.2.5 Other Separation Modes; 1.3 Some Common-Sense Corollaries; 1.4 How to Get More Information; 1.5 Summary; 1.6 References; 2 Basic Terms and Concepts; 2.1 Scope; 2.2 Basic Terms and Concepts 327 $a2.2.1 Retention Time (t(R)), Void Time (t(M)), Peak Height (h), and Peak Width (w(b))2.2.2 Retention Volume (V(R)), Void Volume (V(M)), and Peak Volume; 2.2.3 Retention Factor (k); 2.2.4 Separation Factor (a); 2.2.5 Column Efficiency and Plate Number (N); 2.2.6 Peak Volume; 2.2.7 Height Equivalent to a Theoretical Plate or Plate Height (HETP or H); 2.2.8 Resolution (R(s)); 2.2.9 Peak Symmetry: Asymmetry Factor (A(s)) and Tailing Factor (T(f)); 2.3 Mobile Phase; 2.3.1 General Requirements; 2.3.2 Solvent Strength and Selectivity; 2.3.3 Buffers; 2.3.4 Acidic Mobile Phases 327 $a2.3.5 Ion-Pairing Additives2.3.6 High pH Mobile Phase; 2.3.7 Other Operating Parameters: Flow Rate (F) and Column Temperature (T); 2.4 The Resolution Equation; 2.5 The Van Deemter Equation; 2.6 Isocratic vs. Gradient Analysis; 2.6.1 Peak Capacity (n); 2.6.2 Key Gradient Parameters (Initial and Final Solvent Strength, Gradient Time [t(G)], and Flow Rate); 2.6.3 The 0.25Dt(G) Rule:When Is Isocratic Analysis More Appropriate?; 2.7 Concept of Orthogonality; 2.8 Sample Capacity; 2.9 Glossary of HPLC Terms; 2.10 Summary and Conclusion; 2.11 References; 3 HPLC Columns and Trends; 3.1 Scope 327 $a3.2 General Column Description and Characteristics 3.2.1 Column Hardware-Standard vs. Cartridge Format; 3.3 Column Types; 3.3.1 Types Based on Chromatographic Modes; 3.3.2 Types Based on Dimensions; 3.3.3 Column Length (L); 3.4 Column Packing Characteristics; 3.4.1 Support Type; 3.4.2 Particle Size (d(p)); 3.4.3 Surface Area and Pore Size (d(pore)); 3.4.4 Bonding Chemistries; 3.4.5 Some General Guidelines for Bonded Phase Selection; 3.5 Modern HPLC Column Trends; 3.5.1 High-Purity Silica; 3.5.2 Hybrid Particles; 3.5.3 Novel Bonding Chemistries; 3.5.4 Fast LC; 3.5.5 Micro LC; 3.5.6 Monoliths 327 $a3.6 Guard Columns3.7 Specialty Columns; 3.7.1 Bioseparation Columns; 3.7.2 Chiral Columns; 3.7.3 Application-Specific Columns; 3.8 Column Selection Guides; 3.9 Summary; 3.10 References; 3.11 Internet Resources; 4 HPLC Instrumentation and Trends; 4.1 Introduction; 4.1.1 Scope; 4.1.2 HPLC Systems and Modules; 4.2 HPLC Solvent Delivery Systems; 4.2.1 High-Pressure and Low-Pressure Mixing Designs in Multisolvent Pumps; 4.2.2 System Dwell Volume; 4.2.3 Trends; 4.3 Injectors and Autosamplers; 4.3.1 Operating Principles of Autosamplers; 4.3.2 Performance Characteristics and Trends; 4.4 Detectors 327 $a4.5 UV/VIS Absorbance Detectors 330 $aA comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include:HPLC operation