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010   $a9786610541713
010   $a0-471-45911-9
010   $a0-471-22394-8
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100   $a20010827d2001      uy             0
101 0 $aeng
135   $aur|n|---|||||
181   $ctxt
182   $cc
183   $acr
200 10$aFlow cytometry$b[electronic resource] $efirst principles /$fAlice Longobardi Givan
205   $a2nd ed.
210   $aNew York $cWiley-Liss$dc2001
215   $a1 online resource (292 p.)
300   $aDescription based upon print version of record.
311   $a0-471-38224-8 
320   $aIncludes bibliographical references (p. 229-231) and index.
327   $aContents; Preface; Acknowledgments (First Edition); Acknowledgments (Second Edition); 1 The Past as Prologue; 2 Setting the Scene; 3 Instrumentation: Into the Black Box; Illumination of the Stream; Centering Cells in the Illuminating Beam; Detection of Signals from Cells; Electronics; 4 Information: Harnessing the Data; Data Storage; Data Analysis; 5 Seeing the Light: Lasers, Fluorochromes, and Filters; General Theory; Lasers; Fluorochromes; Partitioning the Signal with Lenses, Filters, and Mirrors; Spectral Compensation
327   $a6 Cells from Without: Leukocytes, Surface Proteins, and the Strategy of GatingCells from Blood; Staining for Surface Markers; Controls; Quantitation; Sensitivity; The Strategy of Gating; Gating on Fluorescence; 7 Cells from Within: Intracellular Proteins; Methods for Permeabilizing Cells; Examples of Intracellular Staining; 8 Cells from Within: DNA in Life and Death; Fluorochromes for DNA Analysis; Ploidy; Cell Cycle Analysis; Two-Color Analysis for DNA and Another Parameter; Chromosomes; Apoptosis; Necrosis; 9 The Sorting of Cells; Sorting Theory; Characterization of Sorted Cells
327   $aAlternative Methods for SortingThe Condition of Cells After Sorting; 10 Disease and Diagnosis: The Clinical Laboratory; The Hematology Laboratory; The Pathology Laboratory; Solid Organ Transplantation; Comments; 11 Out of the Mainstream: Research Frontiers; Functional Assays; The Aquatic Environment; Reporter Molecules; Microbiology; Molecular Biology; Multiplex Cytometry for Soluble Analytes; Reproductive Technology; 12 Flowing On: The Future; General References; Glossary; A; B; C; D; E; F; G; H; I; L; M; N; O; P; Q; R; S; T; V; W; Figure Credits; Index; A; B; C; D; E; F; G; H; I; J; K; L; M
327   $aNO; P; Q; R; S; T; U; V; W; X; Y; Z
330   $aFlow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical a
606   $aFlow cytometry
606   $aCytology
608   $aElectronic books.
615  0$aFlow cytometry.
615  0$aCytology.
676   $a571.6/028
676   $a571.60282
700   $aGivan$b Alice Longobardi$0950597
801  0$bMiAaPQ
801  1$bMiAaPQ
801  2$bMiAaPQ
906   $aBOOK
912   $a9910143186703321
996   $aFlow cytometry$92149266
997   $aUNINA