LEADER 05379nam 22006734a 450 001 9910782618103321 005 20230617003216.0 010 $a1-282-11753-X 010 $a9786612117534 010 $a1-4443-1128-X 035 $a(CKB)1000000000719771 035 $a(EBL)428059 035 $a(OCoLC)476272225 035 $a(SSID)ssj0000364736 035 $a(PQKBManifestationID)11268001 035 $a(PQKBTitleCode)TC0000364736 035 $a(PQKBWorkID)10399033 035 $a(PQKB)11726039 035 $a(MiAaPQ)EBC428059 035 $a(Au-PeEL)EBL428059 035 $a(CaPaEBR)ebr10304025 035 $a(CaONFJC)MIL211753 035 $a(EXLCZ)991000000000719771 100 $a20020427d2003 uy 0 101 0 $aeng 135 $aurcn||||||||| 181 $ctxt 182 $cc 183 $acr 200 10$aPrinciples of genome analysis and genomics$b[electronic resource] /$fSandy B. Primrose, Richard M. Twyman 205 $a3rd ed. 210 $aMalden, Mass. $cBlackwell Pub.$d2003 215 $a1 online resource (272 p.) 300 $aDescription based upon print version of record. 311 $a1-4051-0120-2 320 $aIncludes bibliographical references (p. [230]-254) and index. 327 $aPreface; Abbreviations; CHAPTER 1 Setting the scene: the new science of genomics; Introduction; Physical mapping of genomes; Sequencing whole genomes; Benefits of genome sequencing; Outline of the rest of the book; Terminology; Keeping up to date; Suggested reading; Useful websites; CHAPTER 2 The organization and structure of genomes; Introduction; Genome size; Sequence complexity; Introns and exons; Genome structure in viruses and prokaryotes; The organization of organelle genomes; The organization of nuclear DNA in eukaryotes; Suggested reading; Useful websites 327 $aCHAPTER 3 Subdividing the genome Introduction; Fragmentation of DNA with restriction enzymes; Separating large fragments of DNA; Isolation of chromosomes; Chromosome microdissection; Vectors for cloning DNA; Yeast artificial chromosomes; P1-derived and bacterial artificial chromosomes as alternatives to yeast artificial chromosomes; Retrofitting; Choice of vector; Suggested reading; Useful website; CHAPTER 4 Assembling a physical map of the genome; Introduction; Restriction enzyme fingerprinting; Marker sequences; Hybridization assays; Physical mapping without cloning 327 $aIntegration of different mapping methods Suggested reading; Useful websites; CHAPTER 5 Sequencing methods and strategies; Basic DNA sequencing; Modifications of chain-terminator sequencing; Automated DNA sequencing; DNA sequencing by capillary array electrophoresis; Base calling and sequence accuracy; High throughput sequencing; Sequencing strategies; Alternative DNA sequencing methodologies; Suggested reading; Useful websites; CHAPTER 6 Genome annotation and bioinformatics; Introduction; Traditional routes to gene identification; Databases; Overview of sequence analysis 327 $aDetecting open-reading frames Software programs for finding genes; Using homology to find genes; Analysis of non-coding RNA and extragenic DNA; Identifying the function of a new gene; Secondary databases of functional domains; Gene ontology; Analyses not based on homology; Genome annotation; Molecular phylogenetics; Suggested reading; Useful websites; CHAPTER 7 Comparative genomics; Introduction; Orthologues,paralogues and gene displacement; Protein evolution by exon shuffing; Comparative genomics of prokaryotes; Comparative genomics of organelles; Comparative genomics of eukaryotes 327 $aOther aspects of comparative genomics Suggested reading; Useful websites; CHAPTER 8 Protein structural genomics; Introduction; Determining gene function by sequence comparison; Determining gene function through conserved protein structure; Approaches to protein structural genomics; Suggested reading; Useful website; CHAPTER 9 Global expression pro ling; Introduction; Traditional approaches to expression pro ling; Global analysis of RNA expression; Global analysis of protein expression; Suggested reading; Useful websites; CHAPTER 10 Comprehensive mutant libraries; Introduction 327 $aHigh-throughput systematic gene knockout 330 $aWith the first draft of the human genome project in the public domain and full analyses of model genomes now available, the subject matter of 'Principles of Genome Analysis and Genomics' is even 'hotter' now than when the first two editions were published in 1995 and 1998. 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