LEADER 05364nam 2200637Ia 450 001 9910142493503321 005 20170815114948.0 010 $a1-280-27011-X 010 $a9786610270118 010 $a0-470-31452-4 010 $a0-470-85481-2 010 $a0-470-85482-0 035 $a(CKB)111056485599104 035 $a(EBL)141614 035 $a(OCoLC)53831952 035 $a(SSID)ssj0000215062 035 $a(PQKBManifestationID)11201993 035 $a(PQKBTitleCode)TC0000215062 035 $a(PQKBWorkID)10167455 035 $a(PQKB)11534882 035 $a(MiAaPQ)EBC141614 035 $a(EXLCZ)99111056485599104 100 $a20020529d2002 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aOn-line LC-NMR and related techniques$b[electronic resource] /$fedited by Klaus Albert 210 $aNew York $cJ. Wiley & Sons$dc2002 215 $a1 online resource (308 p.) 300 $aDescription based upon print version of record. 311 $a0-471-49649-9 320 $aIncludes bibliographical references and index. 327 $aOn-Line LC-NMR and Related Techniques; Contents; Contributors; Preface; 1 LC-NMR: Theory and Experiment; 1.1 Introduction; 1.2 NMR in a Flowing Liquid; 1.3 Design of Continuous-Flow NMR Probes; 1.4 Experimental Arrangement for HPLC-(1)H NMR Coupling; 1.5 Practical Considerations, Solvent Suppression Techniques, Gradient Elution and Purity of HPLC Solvents; 1.5.1 Solvent Signal Suppression; 1.5.2 Purity of HPLC-Grade Solvents; References; 2 LC-NMR: Automation; 2.1 Practical Use of LC-NMR and LC-NMR/MS; 2.2 Different Working Modes in LC-NMR; 2.2.1 On-Flow; 2.2.2 Direct Stop-Flow 327 $a2.2.3 Loop Storage/Loop Transfer2.2.4 Conclusions; 2.3 Use of Mass Spectrometry in the Set-Up; 2.4 Measurement Procedures; 2.4.1 Sample Preparation and Introduction ('Injection') into the Chromatography System; 2.4.2 Chromatographic Separation; 2.4.3 Peak Detection and Selection; 2.4.4 Mass Spectrometric Measurements; 2.4.5 Nuclear Magnetic Resonance Measurements; 2.4.6 Sample Recovery; 2.5 Conclusions; References; 3 Biomedical and Pharmaceutical Applications of HPLC-NMR and HPLC-NMR-MS; 3.1 Introduction; 3.2 Technical and Operational Overview; 3.3 Applications in Combinatorial Chemistry 327 $a3.4 Application to Chemical Impurities3.5 Application to Chiral Separations of Pharmaceutical Mixtures; 3.6 Application to Natural Products; 3.7 Application to Chemical Reactivity of Drug Glucuronides; 3.8 Application to Futile Deacetylation Reactions; 3.9 Application to Trapping of Reactive Intermediates; 3.10 Application to Uptake and Transformation of Xenobiotics by Plants; 3.11 Separation of Lipoproteins and their Characterisation using HPLC-NMR; 3.12 Superheated-Water HPLC-NMR and HPLC-NMR-MS Studies on Pharmaceuticals 327 $a3.13 Application of Hypernation to a Mixture of Non-Steroidal Anti-Inflammatory Drugs3.14 Concluding Remarks; References; 4 Application of On-Line LC-NMR and Related Techniques to Drug Metabolism Studies; 4.1 Introduction; 4.2 LC-NMR Techniques; 4.2.1 Continuous-Flow LC-NMR; 4.2.2 Time-Slice LC-NMR; 4.2.3 Stop-Flow LC-NMR; 4.2.4 Loop-Storage; 4.2.5 LC-NMR-MS; 4.3 Application of LC-NMR-MS to Drug Metabolism: The Structure Elucidation of Rat Urinary Metabolites of Efavirenz by LC-NMR-MS; 4.3.1 Experimental; 4.3.2 Results; 4.4 Conclusions; References; 5 LC-NMR for Natural Products Analysis 327 $a5.1 Application of LC-NMR and LC-NMR-MS Hyphenation to Natural Products Analysis5.1.1 Introduction; 5.1.1.1 General Aspects; 5.1.1.2 Applications; 5.1.2 Application of LC-NMR-MS to Glycosidic Natural Products of Marine Origin; 5.1.2.1 Introduction - Need for LC-NMR; 5.1.2.2 Methodology: On-Flow LC-NMR-MS Screening; 5.1.2.3 NMR - Structural Information; 5.1.2.4 Mass Spectrometry and D-H Back-Exchange Experiments; 5.1.2.5 Stop-Flow Experiments; 5.1.2.6 Complimentary Structural Information of NMR and MS; 5.1.2.7 Conclusions; 5.1.3 Acknowledgements; References 327 $a5.2 Hyphenation of Modern Extraction Techniques to LC-NMR for the Analysis of Geometrical Carotenoid Isomers in Functional Food and Biological Tissues 330 $aThis book gives a comprehensive overview of the basis and the current applications of LC-NMR and related techniques. It deals with the practical aspects of the hardware and software set-up for a successful performance of on-line coupling experiments. It covers the solution of real-word problems from the fields of biomedical, pharmaceutical and environmental studies as well as the analysis of natural products and polymeric compounds. Thus guidelines for an efficient application of the powerful hyphenated technique LC-NMR in combination with LC-MS are presented. Besides LC-NMR, important techniq 606 $aNuclear magnetic resonance spectroscopy 606 $aLiquid chromatography 608 $aElectronic books. 615 0$aNuclear magnetic resonance spectroscopy. 615 0$aLiquid chromatography. 676 $a543.089 676 $a543/.0877 701 $aAlbert$b Klaus$0884205 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910142493503321 996 $aOn-line LC-NMR and related techniques$91974475 997 $aUNINA