LEADER 05321nam 2200553 a 450 001 9910141511403321 005 20200520144314.0 010 $a1-118-52306-7 010 $a1-299-14787-9 010 $a1-118-52318-0 035 $a(CKB)2670000000325732 035 $a(EBL)1119532 035 $a(OCoLC)827208164 035 $a(MiAaPQ)EBC1119532 035 $a(Au-PeEL)EBL1119532 035 $a(CaPaEBR)ebr10653578 035 $a(CaONFJC)MIL446037 035 $a(PPN)224940074 035 $a(EXLCZ)992670000000325732 100 $a20130114d2013 uy 0 101 0 $aeng 135 $aur|n|---||||| 200 00$aProteins in solution and at interfaces$b[electronic resource] $emethods and applications in biotechnology and materials science /$fedited by Juan M. Ruso, A?ngel Pin?eiro 210 $aHoboken, N.J. $cJohn Wiley & Sons Inc.$d2013 215 $a1 online resource (518 p.) 225 0$aWiley series on surface and interfacial chemistry 300 $aTwo columns to the page. 311 $a0-470-95251-2 320 $aIncludes bibliographical references and index. 327 $aPROTEINS IN SOLUTION AND AT INTERFACES; CONTENTS; PREFACE; CONTRIBUTORS; PART I; 1 X-RAY CRYSTALLOGRAPHY OF BIOLOGICAL MACROMOLECULES: FUNDAMENTALS AND APPLICATIONS; 1.1 INTRODUCTION; 1.2 FUNDAMENTALS OF X-RAY DIFFRACTION; 1.2.1 X-Ray Radiation and Interaction with Matter; 1.2.2 Crystals and Symmetry; 1.2.3 Diffraction by Crystals; 1.2.4 Real and Reciprocal Space; 1.2.5 Structure Factors; 1.2.6 Fourier Synthesis and Transform; 1.2.7 The Phase Problem; 1.3 THE STRUCTURE DETERMINATION PROCESS; 1.3.1 Sample Production and Conditioning; 1.3.2 Crystallization; 1.3.3 Data Collection and Processing 327 $a1.3.4 Structure Determination1.3.5 Electron Density Map Interpretation: Model Construction; 1.3.6 Model Refinement; 1.3.7 Validation; 1.4 STRUCTURAL ANALYSIS AND BIOLOGICAL IMPLICATIONS; 1.4.1 Structural Analysis; 1.4.2 Biological Implications; 1.5 FUTURE PROSPECTS; ACKNOWLEDGMENTS; REFERENCES; 2 NUCLEAR MAGNETIC RESONANCE METHODS FOR STUDYING SOLUBLE, FIBROUS, AND MEMBRANE-EMBEDDED PROTEINS; 2.1 INTRODUCTION AND BACKGROUND; 2.1.1 Nuclear Angular Momentum; 2.1.2 Chemical Shifts; 2.1.3 Nuclear Spin Interactions; 2.1.4 Relaxation; 2.1.5 Isotopic Labeling; 2.1.6 Samples; 2.2 STRUCTURAL DATA 327 $a2.2.1 Resonance Assignment2.2.2 Distance Measurements; 2.2.3 Angular Information; 2.2.4 Residual Dipolar Couplings; 2.2.5 Use of Paramagnetic Agents; 2.2.6 Oriented Samples; 2.2.7 Structure Calculations; 2.3 DYNAMICS; 2.3.1 Fast (ps-ns) Motions; 2.3.2 Slow (?s-ms) Motions; 2.3.3 Motions in Solids; 2.4 INTERMOLECULAR INTERACTIONS; 2.4.1 Identification of Interaction Surfaces; 2.4.2 Structural Restraints; 2.5 DISCUSSION; 2.5.1 Large Protein Systems; 2.5.2 Advantages and Disadvantages of Solution and Solid-State MAS NMR; 2.5.3 Complementary Techniques; 2.6 CONCLUSION; REFERENCES 327 $a3 SMALL-ANGLE X-RAY SCATTERING APPLIED TO PROTEINS IN SOLUTION3.1 INTRODUCTION; 3.2 SAXS THEORY; 3.2.1 General Equations; 3.2.2 Isotropy; 3.2.3 Homogeneous Scattering Particles (Two-Phase Model); 3.2.4 Model-Independent Shape Analysis: Kratky's Representation, Guinier's Law, and Porod's Invariant; 3.2.5 Methods to Calculate P(q); 3.2.6 Protein-Protein Interaction: S(q) Evaluation; 3.3 EXAMPLES; 3.3.1 Rg, p(r), Kratky's Representation and Shape Reconstruction: Analysis of Protein TcOYE; 3.3.2 Guinier's Analysis and Multipole Expansion Shape Reconstruction: The Replication Factor C Case 327 $a3.3.3 Partial Folded and Unfolded Protein: Analysis of the Protein Bovine Serum Albumin, BSA, Under the Influence of Denaturing Agents3.4 PROTEIN INTERACTION: BSA CASE; 3.5 PROTEIN AGGREGATION; 3.6 CONCLUSION; ACKNOWLEDGMENTS; REFERENCES; 4 ANALYZING THE SOLUTION STATE OF PROTEIN STRUCTURE, INTERACTIONS, AND LIGANDS BY SPECTROSCOPIC METHODS; 4.1 INTRODUCTION; 4.1.1 Overview; 4.1.2 Protein Secondary Structures and Motifs; 4.1.3 Structure Determination: Spectroscopic Methods; 4.2 ULTRAVIOLET-VISIBLE ABSORPTION SPECTROSCOPY; 4.2.1 Background 327 $a4.2.2 Lambert-Beer Law: Determination of Protein Concentration 330 $a"Proteins in solution and at interfaces are increasingly used in exciting new applications, from biomimetic materials to nanoparticle patterning. This book surveys the state-of-the-art in the field, providing scientists in diverse areas with a comprehensive and modern analysis of the techniques used in protein characterization, as well as insight into the most important applications. Topics include protein and protein aggregate structure; computational and experimental techniques for the study of protein structure, aggregation, and adsorption; proteins in non-standard conditions; and biotechnological applications"--$cProvided by publisher. 410 0$aWiley Series on Surface and Interfacial Chemistry 606 $aProteins$xBiotechnology 615 0$aProteins$xBiotechnology. 676 $a660.6/3 701 $aRuso$b Juan M$g(Ruso Beiras, Juan Manuel)$0992617 701 $aPin?eiro$b A?ngel$f1973-$0992618 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910141511403321 996 $aProteins in solution and at interfaces$92272913 997 $aUNINA