LEADER 05076nam 2200601 a 450 001 9910141394503321 005 20170814180632.0 010 $a3-527-65594-8 010 $a3-527-65597-2 010 $a1-299-17876-6 010 $a3-527-65596-4 035 $a(CKB)2670000000262894 035 $a(EBL)1023285 035 $a(OCoLC)815389174 035 $a(SSID)ssj0000741374 035 $a(PQKBManifestationID)12281304 035 $a(PQKBTitleCode)TC0000741374 035 $a(PQKBWorkID)10720739 035 $a(PQKB)11064915 035 $a(MiAaPQ)EBC1023285 035 $a(EXLCZ)992670000000262894 100 $a20130222d2012 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aLipidomics$b[electronic resource] $etechnologies and applications /$fedited by Kim Ekroos 210 $aWeinheim $cWiley-VCH$d2012 215 $a1 online resource (358 p.) 300 $aDescription based upon print version of record. 311 $a3-527-33098-4 320 $aIncludes bibliographical references and index. 327 $aLipidomics: Technologies and Applications; Contents; Preface; List of Contributors; 1 Lipidomics Perspective: From Molecular Lipidomics to Validated Clinical Diagnostics; 1.1 Introduction; 1.2 Hierarchical Categorization of the Analytical Lipid Outputs; 1.2.1 Lipid Class; 1.2.2 Sum Compositions; 1.2.3 Molecular Lipids; 1.2.4 Structurally Defined Molecular Lipids; 1.3 The Type of Lipid Information Delivers Different Biological Knowledge; 1.4 Untying New Biological Evidences through Molecular Lipidomic Applications; 1.5 Molecular Lipidomics Approaches Clinical Diagnostics 327 $a1.6 Current Roadblocks in Lipidomics1.7 Conclusions; References; 2 Lipids in Cells; 2.1 Introduction; 2.2 Basis of Cellular Lipid Distribution; 2.3 Lipid Distribution by Nonvesicular Routes; 2.4 Lipids in Different Cell Types; 2.5 Functional Implications of Membrane Lipid Composition; 2.6 Outlook: Collectives and Phase Separation; References; 3 High-Throughput Molecular Lipidomics; 3.1 Introduction; 3.2 Lipid Diversity; 3.3 Function of Molecular Lipids; 3.4 Automated Sample Preparation; 3.5 Different Approaches to Molecular Lipidomics; 3.5.1 Untargeted versus Targeted Approaches 327 $a3.5.2 Shotgun Lipidomics3.5.3 Analytical Validation of the Shotgun Approach; 3.5.4 Targeted LC-MS Lipidomics; 3.6 Data Processing and Evaluation; 3.7 Lipidomic Workflows; 3.8 Conclusions and Future Perspectives; References; 4 Multidimensional Mass Spectrometry-Based Shotgun Lipidomics; 4.1 Introduction; 4.2 Multidimensional Mass Spectrometry-Based Shotgun Lipidomics; 4.2.1 Intrasource Separation; 4.2.2 The Principle of Multidimensional Mass Spectrometry; 4.2.3 Variables in Multidimensional Mass Spectrometry; 4.2.3.1 Variables in Fragment Monitoring by Tandem MS Scans 327 $a4.2.3.2 Variables Related to the Infusion Conditions4.2.3.3 Variables under Ionization Conditions; 4.2.3.4 Variables under Collision Conditions; 4.2.3.5 Variables Related to the Sample Preparations; 4.3 Application of Multidimensional Mass Spectrometry-Based Shotgun Lipidomics for Lipidomic Analysis; 4.3.1 Identification of Lipid Molecular Species by 2D Mass Spectrometry; 4.3.1.1 Identification of Anionic Lipids; 4.3.1.2 Identification of Weakly Anionic Lipids; 4.3.1.3 Identification of Charge Neutral but Polar Lipids; 4.3.1.4 Identification of Sphingolipids 327 $a4.3.1.5 The Concerns of the MDMS-Based Shotgun Lipidomics for Identification of Lipid Species4.3.2 Quantification of Lipid Molecular Species by MDMS-Based Shotgun Lipidomics; 4.3.2.1 The Principle of Quantification of Individual Lipid Species by MS; 4.3.2.2 Quantification by Using a Two-Step Procedure in MDMS-Based Shotgun Lipidomics; 4.3.2.3 Quantitative Analysis of PEX7 Mouse Brain Lipidome by MDMS-Based Shotgun Lipidomics; 4.4 Conclusions; References; 5 Targeted Lipidomics: Sphingolipidomics; 5.1 Introduction; 5.2 Sphingolipids Description and Nomenclature 327 $a5.3 Sphingolipids Analysis via Targeted LC-MS/MS 330 $aFocusing on the practical applications, this user-oriented guide presents current technologies and strategies for systems-level lipid analysis, going beyond basic research to concentrate on commercial uses of lipidomics in biomarker and diagnostic development, as well as within pharmaceutical drug discovery and development. The editor and authors have experience of the most recent analytical instruments and techniques, allowing them to provide here first-hand practical experience for newcomers to the field. The first half of the book covers current methodologies, ranging from global to tar 606 $aLipids$xAnalysis 608 $aElectronic books. 615 0$aLipids$xAnalysis. 676 $a572.57 676 $a612.015 701 $aEkroos$b Kim$0867757 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910141394503321 996 $aLipidomics$91936850 997 $aUNINA