LEADER 05744nam 2200769Ia 450 001 9910141379403321 005 20240717093125.0 010 $a1-283-64427-4 010 $a1-118-38290-0 010 $a1-118-38291-9 035 $a(CKB)2670000000260760 035 $a(EBL)918267 035 $a(OCoLC)781078019 035 $a(SSID)ssj0000719028 035 $a(PQKBManifestationID)11421207 035 $a(PQKBTitleCode)TC0000719028 035 $a(PQKBWorkID)10752934 035 $a(PQKB)10413425 035 $a(SSID)ssj0000718953 035 $a(PQKBManifestationID)11477576 035 $a(PQKBTitleCode)TC0000718953 035 $a(PQKBWorkID)10752749 035 $a(PQKB)11249828 035 $a(MiAaPQ)EBC918267 035 $a(Au-PeEL)EBL918267 035 $a(CaPaEBR)ebr10606068 035 $a(CaONFJC)MIL395677 035 $a(PPN)177056142 035 $a(EXLCZ)992670000000260760 100 $a20120306d2012 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aFundamentals of light microscopy and electronic imaging /$fDouglas B. Murphy, Michael W. Davidson 205 $a2nd ed. 210 $aHoboken, N.J. $cWiley-Blackwell$d2012 215 $a1 online resource (xiii, 538 p.) 300 $aDescription based upon print version of record. 311 $a0-471-69214-X 320 $aIncludes bibliographical references and index. 327 $aTitle page; Copyright page; Contents; Preface; Acknowledgments; CHAPTER 1: Fundamentals of Light Microscopy; Overview; Optical Components of the Light Microscope; Aperture and Image Planes in a Focused, Adjusted Microscope; Koehler Illumination; Adjusting the Microscope for Koehler Illumination; Fixed Tube Length versus Infinity Optical Systems; Precautions for Handling Optical Equipment; Care and Maintenance of the Microscope; CHAPTER 2: Light and Color; Overview; Light as a Probe of Matter; The Dual Particle- and Wave-Like Nature of Light; The Quality of Light 327 $aProperties of Light Perceived by the EyePhysical Basis for Visual Perception and Color; Addition and Subtraction Colors; CHAPTER 3: Illuminators, Filters, and the Isolation of Specific Wavelengths; Overview; Illuminators and Their Spectra; Illuminator Alignment and Bulb Replacement; Filters for Adjusting the Intensity and Wavelength of Illumination; Neutral Density Filters; Colored Glass Filters; Interference Filters; Effects of Light on Living Cells; CHAPTER 4: Lenses and Geometrical Optics; Overview; Reflection and Refraction of Light; Image Formation by a Simple Lens 327 $aRules of Ray Tracing for a Simple LensObject-Image Math; The Principal Aberrations of Lenses; Designs and Specifications of Objectives; Special Objective Designs; Markings on the Barrel of an Objective; Image Brightness; Objective Parfocality; Condensers; Oculars; Microscope Slides and Coverslips; The Care and Cleaning of Optics; Dust; Immersion Oil; Scratches and Abrasions; Mechanical Force; CHAPTER 5: Diffraction and Interference in Image Formation; Overview; Diffraction and Interference; The Diffraction Image of a Point Source of Light 327 $aThe Constancy of Optical Path Length between Object and ImageEffect of Aperture Angle on Diffraction Spot Size; Diffraction by a Grating and Calculation of Its Line Spacing, D; Abbe?'s Theory for Image Formation in the Microscope; A Diffraction Pattern Is Formed in the Rear Aperture of the Objective; Preservation of Coherence: Essential Requirement for Image Formation; CHAPTER 6: Diffraction and Spatial Resolution; Overview; Numerical Aperture; Spatial Resolution; Depth of Field and Depth of Focus; Optimizing the Microscope Image: A Compromise between Spatial Resolution and Contrast 327 $aCHAPTER 7: Phase Contrast Microscopy and Darkfield MicroscopyOverview; Phase Contrast Microscopy; The Behavior of Waves from Phase Objects in Brightfield Microscopy; Wave Terminology and the Importance of Coherence; Depiction of Wave Interactions with Sine Wave and Vector Diagrams; The Role of Differences in Optical Path Lengths; The Optical Design of the Phase Contrast Microscope; Alignment; Interpreting the Phase Contrast Image; Darkfield Microscopy; Theory and Optics; Image Interpretation; CHAPTER 8: Properties of Polarized Light; Overview; The Generation of Polarized Light 327 $aPolarization by Reflection and Scattering 330 $aFundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.The book is divided into three sections covering opti 606 $aMicroscòpia$2lemac 606 $aImatges$xProcessament$xTècniques digitals$2lemac 606 $aMicroscopy 606 $aImage processing$xDigital techniques 615 7$aMicroscòpia 615 7$aImatges$xProcessament$xTècniques digitals 615 0$aMicroscopy. 615 0$aImage processing$xDigital techniques. 676 $a502.8/2 700 $aMurphy$b Douglas B$0871589 701 $aDavidson$b Michael W$g(Michael Wesley),$f1950-$0871590 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910141379403321 996 $aFundamentals of light microscopy and electronic imaging$91945611 997 $aUNINA