LEADER 05531nam 2200697 450 001 9910140501703321 005 20200520144314.0 010 $a1-78684-221-1 010 $a1-118-87090-5 010 $a1-118-87091-3 010 $a1-118-87092-1 035 $a(CKB)2670000000570321 035 $a(EBL)1810512 035 $a(SSID)ssj0001348461 035 $a(PQKBManifestationID)11813278 035 $a(PQKBTitleCode)TC0001348461 035 $a(PQKBWorkID)11362928 035 $a(PQKB)11355372 035 $a(MiAaPQ)EBC1810512 035 $a(Au-PeEL)EBL1810512 035 $a(CaPaEBR)ebr10949732 035 $a(CaONFJC)MIL649406 035 $a(OCoLC)892799105 035 $a(PPN)19821572X 035 $a(EXLCZ)992670000000570321 100 $a20141016h20152015 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aVaccine development and manufacturing /$fedited by Emily P. Wen, Ronald Ellis, Narahari S. Pujar ; Michel Chartrain [and thirty one others], contributors 210 1$aHoboken, New Jersey :$cWiley,$d2015. 210 4$dİ2015 215 $a1 online resource (1044 p.) 225 1 $aWiley Series in Biotechnology and Bioengineering 300 $aDescription based upon print version of record. 311 $a0-470-26194-3 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aCover; Series; Title Page; Copyright; Acknowledgments; Preface; Contributors; Chapter 1: History of Vaccine Process Development; 1.1 Introduction; 1.2 Vaccines Bioprocess Evolution; 1.3 Live Attenuated and Inactivated Virus Vaccines; 1.4 Live or Whole-Killed Bacterial Vaccines; 1.5 Classical Subunit Vaccines; 1.6 Recombinant Subunit Vaccines; 1.7 Conjugate Vaccines; 1.8 Downstream Processing; 1.9 Vaccines for the Developing World: Large Volume, Low Cost, and Thermostable; 1.10 Summary; Acknowledgments; References 327 $aChapter 2: The Production of Plasmid DNA Vaccine in Escherichia coli: A Novel Bacterial-Based Vaccine Production Platform2.1 Introduction: E. coli in Vaccine Production; 2.2 Brief Overview of DNA Vaccines: Mechanisms and Methods of Vaccinations; 2.3 Current Status of DNA Vaccines; 2.4 Required Physical Properties of Plasmid DNA Vaccines; 2.5 Choice of E. coli Host Strain; 2.6 Factors Influencing Plasmid Stability; 2.7 Transformation, Selection of Producing Clones, and Cell Banking; 2.8 Production Process; 2.9 Requirements for Clinical Supplies; 2.10 Conclusions; References 327 $aChapter 3: Fungal Expression Systems for Vaccine Production3.1 Introduction; 3.2 Hepatitis B Vaccines; 3.3 Human Papillomavirus Vaccine; 3.4 Malaria Vaccine Candidates; 3.5 HIV Vaccine Candidates; 3.6 Veterinary Vaccines; 3.7 Perspectives; 3.8 Concluding Remarks; Acknowledgments; References; Chapter 4: Novel Expression Systems for Vaccine Production; 4.1 Introduction; 4.2 Subunit Vaccines; 4.3 Expression Systems; 4.4 Novel Expression Systems; 4.5 Production of Recombinant Proteins in Plants; 4.6 Launch Vector System; 4.7 Conclusions; References; Chapter 5: Viral Vaccines Purification 327 $a5.1 Introduction5.2 Process Tasks; 5.3 Conclusions and Outlook; Acknowledgments; Nomenclature; Abbreviations; References; Chapter 6: Protein Subunit Vaccine Purification; 6.1 Introduction; 6.2 Purification Technologies-Applications in Protein Subunit Vaccine Purification; 6.3 Purification Process Development and Scale-Up for Protein Subunit Vaccine; 6.4 Process Definition Studies; 6.5 Process Economy and Automation; 6.6 Application of Process Analytical Technology in Protein Purification; 6.7 Downstream Purification-An Outlook; References; Chapter 7: Conjugate Vaccine Production Technology 327 $a7.1 Conjugate Vaccine Production Technology7.2 Preparation of Antigen and Carrier Protein; 7.3 Polysaccharide Size; 7.4 Activation and Coupling of Polysaccharide and Carrier Protein; 7.5 Characterization of the Conjugate; 7.6 Future Directions; References; Chapter 8: Stabilization and Formulation of Vaccines; 8.1 Introduction; 8.2 An Example of a Modern Vaccine Characterization Strategy; 8.3 A Comprehensive Approach to Vaccine Formulation in Practice; 8.4 Conclusions; References; Chapter 9: Lyophilization In Vaccine Processes; 9.1 Introduction; 9.2 Formulation; 9.3 Filling; 9.4 Lyophilization 327 $a9.5 Equipment 330 $aVaccine Manufacturing and Production is an invaluable reference on how to produce a vaccine - from beginning to end - addressing all classes of vaccines from a processing, production, and regulatory viewpoint. It will provide comprehensive information on the various fields involved in the production of vaccines, from fermentation, purification, formulation, to regulatory filing and facility designs. In recent years, there have been tremendous advances in all aspects of vaccine manufacturing. Improved technology and growth media have been developed for the production of cell culture with high 410 0$aWiley series in biotechnology and bioengineering. 606 $aVaccines$vLaboratory manuals 615 0$aVaccines 676 $a615.3/72 702 $aWen$b Emily P. 702 $aEllis$b Ronald J. 702 $aPujar$b Narahari S. 702 $aChartrain$b Michel 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910140501703321 996 $aVaccine development and manufacturing$92121251 997 $aUNINA