LEADER 04859nam 2200613Ia 450 001 9910140015103321 005 20170918181121.0 010 $a1-282-46084-6 010 $a9786612460845 010 $a3-527-62665-4 010 $a3-527-62664-6 035 $a(CKB)1000000000820644 035 $a(EBL)481899 035 $a(OCoLC)566109955 035 $a(SSID)ssj0000342913 035 $a(PQKBManifestationID)11252510 035 $a(PQKBTitleCode)TC0000342913 035 $a(PQKBWorkID)10288879 035 $a(PQKB)10955362 035 $a(MiAaPQ)EBC481899 035 $a(PPN)198274629 035 $a(EXLCZ)991000000000820644 100 $a20071213d2009 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aSingle cell analysis$b[electronic resource] $etechnologies and applications /$fedited by Dario Anselmetti 210 $aWeinheim $cWiley-VCH$dc2009 215 $a1 online resource (286 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31864-X 320 $aIncludes bibliographical references and index. 327 $aSingle Cell Analysis: Technologies and Applications; Contents; Foreword; Preface; List of Contributors; Part I Single Cell Analysis: Imaging; 1 Single Molecule Fluorescence Monitoring in Eukaryotic Cells: Intranuclear Dynamics of Splicing Factors; 1.1 Motivation; 1.2 Experimental Approach; 1.3 Single Particle Tracking within Living Cells; 1.4 Pre-Messenger RNA Splicing; 1.5 Intranuclear Splicing Factor Tracking; 1.6 Intranuclear U1 snRNP Splicing Factor Binding; 1.7 Events in Speckles; 1.8 Intranuclear U1 snRNP Mobility; 1.9 Perspectives of Single Molecule Microscopy; References 327 $a2 Gene Classification and Quantitative Analysis of Gene Regulation in Bacteria using Single Cell Atomic Force Microscopy and Single Molecule Force Spectroscopy2.1 Introduction; 2.2 AFM on Paracrystalline Cell Surface Layers of C. glutamicum: Protein Sequence Information and Morphology; 2.3 Imaging of Living C. glutamicum Cells with Molecular Resolution: Genes, Transcriptional Regulation and Morphology; 2.4 Single Molecule Force Spectroscopy on Specific Protein-DNA Complexes: Transcriptional Regulation in S. meliloti 327 $a2.5 Effector-Induced Protein-DNA Binding on the Single Molecule Level: Quorum Sensing in S. meliloti2.6 Conclusion; References; 3 Cellular Cryo-Electron Tomography (CET): Towards a Voyage to the Inner Space of Cells; 3.1 Introduction; 3.2 Tomography with the Electron Microscope - a Practical Perspective; 3.2.1 Sample Preparation; 3.2.2 Instrumental and Technical Requirements; 3.2.3 Alignment, Reconstruction and Visualization; 3.3 Molecular Interpretation of Cellular Tomograms; 3.4 Outlook: The Future is Bright; References; Part II Single Cell Analysis: Technologies; 4 Single Cell Proteomics 327 $a4.1 Introduction4.2 The Challenge; 4.3 Single Cell Proteomics: Mass Spectrometry; 4.4 Single Cell Separations; 4.5 Ultrasensitive Protein Analysis: Capillary Electrophoresis with Laser-Induced Fluorescence Detection; 4.6 Capillary Sieving Electrophoresis of Proteins from a Single Cancer Cell; 4.7 Cell Cycle-dependent Single Cell Capillary Sieving Electrophoresis; 4.8 Tentative Identification of Proteins in Single Cell Electropherograms; 4.9 Capillary Micellar and Submicellar Separation of Proteins from a Single Cell; 4.10 Two-Dimensional Capillary Electrophoresis of Proteins in a Single Cell 327 $a4.11 Single Copy Detection of Specific Proteins in Single Cells4.12 Conclusion; References; 5 Protein Analysis of Single Cells in Microfluidic Format; 5.1 Introduction; 5.2 Microfluidic Single Cell Analysis Concept; 5.2.1 Single Cell Selection and Trapping; 5.2.2 Single Cell Lysis; 5.3 Single Cell Electrophoretic Separation and Detection of Proteins; 5.3.1 Label-Based Fluorescence Detection; 5.3.2 Label-Free Fluorescence Detection; 5.3.2.1 UV-LIF in Quartz Microfluidic Devices; 5.3.2.2 UV-LIF in PDMS Microfluidic Devices; 5.3.2.3 Single Cell UV-LIF Electrophoretic Analysis 327 $a5.4 Future Directions in Single Cell Analysis 330 $aThe first-ever comprehensive overview of the methods used in this key technology in modern biology provides the latest working knowledge needed by every scientist entering this growing field. It covers all the current technology and application areas, from microscopy and spectroscopy to proteomics and microfluidics. 606 $aCells$xAnalysis 606 $aCytology 615 0$aCells$xAnalysis. 615 0$aCytology. 676 $a571.6 701 $aAnselmetti$b D$0937289 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910140015103321 996 $aSingle cell analysis$92111215 997 $aUNINA