LEADER 05336nam 2200661Ia 450 001 9910139786403321 005 20230421045547.0 010 $a1-282-37168-1 010 $a9786612371684 010 $a1-4443-1361-4 010 $a1-4443-1360-6 035 $a(CKB)1000000000788724 035 $a(EBL)470338 035 $a(OCoLC)647844280 035 $a(SSID)ssj0000239707 035 $a(PQKBManifestationID)11195272 035 $a(PQKBTitleCode)TC0000239707 035 $a(PQKBWorkID)10257413 035 $a(PQKB)11335229 035 $a(MiAaPQ)EBC470338 035 $a(Au-PeEL)EBL470338 035 $a(CaPaEBR)ebr10359443 035 $a(CaONFJC)MIL237168 035 $a(EXLCZ)991000000000788724 100 $a19960702d1997 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aRoute maps in gene technology$b[electronic resource] /$fMatthew R. Walker, with Ralph Rapley 210 $aOxford ;$aMalden, MA $cBlackwell Science$d1997 215 $a1 online resource (336 p.) 300 $aDescription based upon print version of record. 311 $a0-632-03792-X 320 $aIncludes bibliographical references (p. 276-320) and index. 327 $aROUTE MAPS IN GENE TECHNOLOGY; CONTENTS; PREFACE; ABOUT THE ROUTE MAPS FORMAT; 1. THE CONCEPT OF GENES IS DEVELOPED; 2. GENES ARE LOCATED TO CHROMOSOMES; 3. GENES ARE COMPOSED OF DNA; 4. THE CHEMICAL BUILDING BLOCKS OF NUCLEIC ACIDS; 5. FORMATION OF THE DNA DOUBLE HELIX; 6. PACKAGING OF DNA WITHIN CELLS; 7. CHROMATIN STRUCTURE AND THE FUNCTIONAL ACTIVITY OF GENES; 8. TYPES AND FUNCTIONS OF DNA-PROTEIN INTERACTIONS; 9. ORGANISATION OF GENOMES INTO MULTIPLE CHROMOSOMES; 10. DISTRIBUTION OF NUCLEIC ACIDS WITHIN EUKARYOTIC CELLS; 11. TYPES OF RNA MOLECULES 327 $a12. THE ANATOMY OF EUKARYOTIC CHROMOSOMES13. ORGANISATION OF GENES WITHIN CHROMOSOMES; 14. THE MOLECULAR ANATOMY OF EUKARYOTIC GENES; 15. CHROMOSOME ABERRATIONS AND LINKS TO HUMAN DISEASE; 16. TYPES OF MUTATIONS AND THEIR EFFECTS; 17. FORMS OF CHEMICALLY ALTERED DNA; 18. DNA REPAIR MECHANISMS; 19. LINKAGE ANALYSIS; 20. PEDIGREE ANALYSIS AND MODES OF INHERITANCE; 21. GENES DICTATE THE NATURE OF PROTEINS; 22. THE NATURE OF THE GENETIC CODE; 23. TRANSCRIPTION: FORMING GENETIC MESSAGES; 24. POST-TRANSCRIPTIONAL PROCESSING OF MESSENGER RNA; 25. TRANSFER AND RIBOSOMAL RNA PROCESSING/MODIFICATION 327 $a26. MECHANISMS REGULATING GENE EXPRESSION27. TRANSCRIPTIONAL REGULATORY SEQUENCES; 28. OPERONS AND PROKARYOTIC CONTROL OF GENE EXPRESSION; 29. TRANSCRIPTION FACTORS AND GENE EXPRESSION; 30. IN VIVO TRANSLATION: DECODING GENETIC MESSAGES; 31. SEQUENCES INVOLVED IN CELLULAR PROTEIN TARGETING; 32. EUKARYOTIC CELL DIVISION: MITOSIS AND MEIOSIS; 33. MOLECULAR MECHANISMS OF CELL CYCLE CONTR0L; 34. GENETIC RECOMBINATION MECHANISMS; 35. GENE TRANSFER DURING BACTERIAL REPRODUCTION; 36. TRANSPOSABLE GENETIC ELEMENTS: TRANSPOSONS; 37. IN VIVO DNA REPLICATION; 38. GENETIC CONTROL OF DEVELOPMENT 327 $a39. THE NATURAL BIOLOGY OF BACTERIOPHAGES40. BACTERIOPHAGE GENETICS; 41. RECOMBINANT DNA TECHNOLOGY; 42. ENZYMES COMMONLY USED IN MOLECULAR BIOLOGY METHODS; 43. RESTRICTION ENDONUCLEASES; 44. RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; 45. ISOLATION OF NUCLEIC ACIDS FROM CELLS AND TISSUES; 46. VISUALISING NUCLEIC ACIDS; 47. ELECTROPHORESIS OF NUCLEIC ACIDS; 48. IN VITRO HYBRIDISATION; 49. TYPES OF HYBRIDISATION ASSAY FORMATS; 50. SOUTHERN BLOTTING; 51. IN SITU HYBRIDISATION; 52. MEASURING TRANSCRIPTIONAL ACTIVITY VIA MESSENGER RNA; 53. CONVERTING MESSENGER RNA INTO COMPLEMENTARY DNA 327 $a54. METHODS FOR DETERMINING DNA NUCLEOTIDE SEQUENCES55. THE POLYMERASE CHAIN REACTION; 56. ALTERNATIVES TO PCR-BASED IN VITRO DNA/RNA AMPLIFICATION; 57. IN VITRO TRANSLATION METHODS; 58. TYPES AND METHODS OF GENE PROBE GENERATION; 59. CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES; 60. TYPES AND APPLICATIONS OF NUCLEOTIDE ANALOGUES; 61. METHODS FOR LABELLING GENE PROBES; 62. FUNDAMENTAL PRINCIPLES OF CLONING; 63. THE NATURE OF CLONING VECTORS; 64. INSERTING FOREIGN DNA INTO VECTORS; 65. THE DEVELOPMENT OF BACTERIOPHAGE VECTORS; 66. PLASMIDS: DEVELOPMENT AS CLONING VECTORS 327 $a67. YEAST-DERIVED PLASMID VECTORS 330 $aRoute Maps in Gene Technology is an exciting new introductory textbook for first-year undergraduates in molecular biology and molecular genetics. The subject is broken down into 140 to 150 key concepts or topics, each of which is dealt with in one doublepaged spread. These range from basic introductory principles to applied topics at the cutting edge of research. A control strip along the top of the page shows the student which pages need to have been read beforehand and which topics may be followed afterward. In addition, at the front of the book are a selection of 'routes,' which the 606 $aGenetic engineering 606 $aHuman genetics 615 0$aGenetic engineering. 615 0$aHuman genetics. 676 $a660.65 676 $a660/.65 700 $aWalker$b Matthew R$0983332 701 $aRapley$b Ralph$0291074 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910139786403321 996 $aRoute maps in gene technology$92244750 997 $aUNINA