LEADER 05540nam 2200757Ia 450 001 9910139211803321 005 20200520144314.0 010 $a9786612689130 010 $a9781282689138 010 $a1282689134 010 $a9780470686522 010 $a0470686529 010 $a9780470686515 010 $a0470686510 035 $a(CKB)2560000000011301 035 $a(EBL)537377 035 $a(OCoLC)638860459 035 $a(SSID)ssj0000425365 035 $a(PQKBManifestationID)11268068 035 $a(PQKBTitleCode)TC0000425365 035 $a(PQKBWorkID)10364442 035 $a(PQKB)10668169 035 $a(MiAaPQ)EBC537377 035 $a(Au-PeEL)EBL537377 035 $a(CaPaEBR)ebr10392951 035 $a(CaONFJC)MIL268913 035 $a(OCoLC)494276896 035 $a(FINmELB)ELB178567 035 $a(Perlego)2773065 035 $a(EXLCZ)992560000000011301 100 $a20091222d2010 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aPlant cell culture $eessential methods /$fMichael R. Davey and Paul Anthony 205 $a1st ed. 210 $aChichester, West Sussex, UK ;$aHoboken, NJ $cWiley-Blackwell$dc2010 215 $a1 online resource (359 p.) 300 $aDescription based upon print version of record. 311 08$a9780470686485 311 08$a0470686480 327 $aPlant Cell Culture; Contents; Preface; Contributors; 1 Plant Micropropagation; 1.1 Introduction; 1.2 Methods and approaches; 1.2.1 Explants and their surface disinfection; 1.2.2 Culture media and their preparation; 1.2.3 Stages of micropropagation; 1.2.4 Techniques of micropropagation; 1.3 Troubleshooting; References; 2 Thin Cell Layers: The Technique; 2.1 Introduction; 2.2 Methods and approaches; 2.2.1 TCL; 2.2.2 Choice of material: Cymbidium hybrid; 2.3 Troubleshooting; 2.3.1 General comments; References; 3 Plant Regeneration - Somatic Embryogenesis; 3.1 Introduction 327 $a3.2 Methods and approaches3.2.1 Selection of the cultivar and type of explant; 3.2.2 Culture media; 3.2.3 Preparation of culture media; 3.2.4 Sterilization of tissues and sterile technique; 3.2.5 Culture and growth of tissue; 3.2.6 Culture and induction of somatic embryos; 3.2.7 Embryo development; 3.2.8 Transfer to soil - the final stage of regeneration; 3.3 Troubleshooting; References; 4 Haploid Plants; 4.1 Introduction; 4.2 Methods and approaches; 4.2.1 Androgenesis; 4.2.2 Diploidization; 4.3 Troubleshooting; References; 5 Embryo Rescue; 5.1 Introduction; 5.2 Methods and approaches 327 $a5.2.1 Identification of the time and type of barrier in hybridization5.2.2 Isolation of plant material after fertilization; 5.2.3 Culture conditions and media; 5.2.4 Confirmation of hybridity and ploidy; 5.2.5 Conditions for regeneration of embryos to plants; 5.3 Troubleshooting; References; 6 In vitro Flowering and Seed Set: Acceleration of Generation Cycles; 6.1 Introduction; 6.2 Methods and approaches; 6.2.1 Protein legumes [7]; 6.2.2 Arabidopsis thaliana [13]; 6.3 Troubleshooting; References; 7 Induced Mutagenesis in Plants Using Physical and Chemical Agents; 7.1 Introduction 327 $a7.2 Methods and approaches7.2.1 Determination of the optimal doses of mutagens for inducing mutations; 7.3 Troubleshooting; 7.3.1 Factors influencing the outcome of mutagenesis using chemical mutagens; 7.3.2 Factors influencing the outcome of mutagenesis using physical mutagens; 7.3.3 Facts about induced mutations; References; 8 Cryopreservation of Plant Germplasm; 8.1 Introduction; 8.2 Methods and approaches; 8.2.1 Main principles; 8.2.2 Slow (two-step) freezing; 8.2.3 Vitrification; 8.2.4 Encapsulation-dehydration; 8.2.5 DMSO droplet freezing; 8.2.6 Combined methods 327 $a8.2.7 Freezing of cold-hardened buds8.2.8 Freezing of orthodox seeds; 8.2.9 Freezing of pollen and spores; 8.3 Troubleshooting; References; 9 Plant Protoplasts: Isolation, Culture and Plant Regeneration; 9.1 Introduction; 9.2 Methods and approaches; 9.2.1 Protoplast isolation; 9.2.2 Protoplast culture; 9.3 Troubleshooting; References; 10 Protoplast Fusion Technology - Somatic Hybridization and Cybridization; 10.1 Introduction; 10.2 General applications of somatic hybridization; 10.3 Methods and approaches; 10.4 Troubleshooting; References; 11 Genetic Transformation - Agrobacterium 327 $a11.1 Introduction 330 $aThe ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common pr 606 $aPlant cell culture 606 $aPlant tissue culture 615 0$aPlant cell culture. 615 0$aPlant tissue culture. 676 $a571.6/382 686 $aWC 5102$2rvk 686 $aWN 5200$2rvk 700 $aDavey$b M. R$g(Michael Raymond),$f1944-$0871749 701 $aAnthony$b Paul$0521529 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910139211803321 996 $aPlant cell culture$91945986 997 $aUNINA