LEADER 05056nam 2200565 450 001 9910139000003321 005 20170816142656.0 010 $a3-527-67374-1 010 $a3-527-33596-X 010 $a3-527-67373-3 035 $a(CKB)2550000001127042 035 $a(EBL)1443914 035 $a(OCoLC)861080200 035 $a(MiAaPQ)EBC1443914 035 $a(EXLCZ)992550000001127042 100 $a20131029h20142014 uy| 0 101 0 $aeng 135 $aur|n|---||||| 181 $2rdacontent 182 $2rdamedia 183 $2rdacarrier 200 00$aMALDI MS $ea practical guide to instrumentation, methods and applications /$fedited by Franz Hillenkamp and Jasna Peter-Katalinic 205 $a2nd ed. 210 1$aWeinheim :$cWiley Blackwell,$d[2014] 210 4$dİ2014 215 $a1 online resource (477 p.) 300 $aDescription based upon print version of record. 311 $a3-527-33331-2 311 $a1-299-96455-9 320 $aIncludes bibliographical references and index. 327 $aCover; Related Titles; Title page; Copyright page; Contents; Preface to the Second Edition; List of Contributors; 1: The MALDI Process and Method; 1.1 Introduction; 1.2 Analyte Incorporation; 1.3 Absorption of the Laser Radiation; 1.4 The Ablation/Desorption Process; 1.5 Ionization; 1.6 Fragmentation of MALDI Ions; 1.7 MALDI of Noncovalent Complexes; 1.8 The Optimal Choice of Matrix: Sample Preparation; 1.8.1 Surface Preparation; 1.8.2 Anchor Sample Plates; 1.8.3 Matrix Additives and Influence of the Sample Plate Surface; Abbreviations; References; 2: MALDI Mass Spectrometry Instrumentation 327 $a2.1 Introduction2.2 Lasers for MALDI-MS; 2.3 Fragmentation of MALDI Ions; 2.3.1 MALDI at Elevated Pressure; 2.3.2 Tandem Mass Spectrometry of MALDI Ions; 2.4 Mass Analyzers; 2.4.1 Axial TOF Mass Spectrometers; 2.4.2 Reflectron TOF Mass Spectrometers; 2.4.3 Tandem TOF Mass Spectrometers; 2.4.4 Orthogonal TOF Mass Analyzers; 2.4.5 Tandem Mass Spectrometry in oTOF Mass Analyzers; 2.4.6 Ion Detectors and Data Processing in MALDI-TOF Analyzers; 2.5 Fourier Transform Ion Cyclotron Resonance Mass Spectrometers; 2.5.1 Tandem Mass Spectrometry on FTICR Mass Spectrometers 327 $a2.6 Quadrupole Ion Trap Mass Spectrometers2.6.1 RF-Only Ion Guides and LIT Mass Spectrometers; 2.6.2 Tandem Mass Spectrometry on QIT Mass Spectrometers; 2.7 Hybrid Mass Spectrometers; 2.7.1 Quadrupole TOF Mass Spectrometers; 2.7.2 Quadrupole FT Mass Spectrometers; 2.7.3 QIT-TOF Mass Spectrometers; 2.7.4 Ion Mobility oTOF Mass Spectrometers; 2.7.5 Orbitrap; 2.8 Future Directions; Definitions and Acronyms; References; 3: MALDI-MS in Protein Chemistry and Proteomics; 3.1 Introduction; 3.2 Sample Preparation for Protein and Peptide Analysis by MALDI-MS 327 $a3.3 Strategies for Using MALDI-MS in Protein Biochemistry3.3.1 Peptide Mass Mapping of Purified Proteins; 3.3.2 Peptide Sequencing by MALDI-MS/MS; 3.3.3 Analysis of Post-Translational Modifications; 3.4 Applications of MALDI-MS in Proteomics; 3.4.1 Protein Identification by MALDI-MS Peptide Mass Mapping; 3.4.2 Quantitation of Proteins by MALDI-MS; 3.5 Computational Tools for Protein Analysis by MALDI-MS; 3.6 Clinical Applications of MALDI-MS; 3.7 Conclusions; Acknowledgments; References; 4: MALDI-Mass Spectrometry Imaging; 4.1 Introduction 327 $a4.2 History of Mass Spectrometry Imaging (MSI) and Microprobing Techniques4.3 MALDI in Micro Dimensions: Instruments and Mechanistic Differences; 4.4 Visualization of Mass Spectrometric Information; 4.5 Data Processing and Data Exchange; 4.6 Matrix Deposition for High-Resolution Imaging; 4.7 Organisms, Organs, and Tissues: MALDI Imaging at Various Lateral Resolutions; 4.7.1 Phospholipid Analysis; 4.7.2 Peptide Analysis; 4.7.3 Drug Monitoring; 4.8 Whole-Cell and Single-Cell Analysis; 4.8.1 Cellular Analysis; 4.8.2 Individually Isolated Cells; 4.8.3 Direct Cellular and Subcellular Imaging 327 $a4.9 Cell Sorting and Capturing 330 $aThis authoritative book on MALDI MS, now finally available in its second edition and edited by one of its inventors, gives an in-depth description of the many different applications, along with a detailed discussion of the technology itself.Thoroughly updated and expanded, with contributions from key players in the field, this unique book provides a comprehensive overview of MALDI MS along with itspossibilities and limitations.The initial chapters deal with the technology and the instrumental setup, followed by chapters on the use of MALDI MS in protein research (including proteomi 606 $aMatrix-assisted laser desorption-ionization 608 $aElectronic books. 615 0$aMatrix-assisted laser desorption-ionization. 676 $a547.70154365 701 $aHillenkamp$b F$0855863 701 $aPeter-Katalinic?$b Jasna$0855864 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910139000003321 996 $aMALDI MS$91910721 997 $aUNINA