LEADER 04398nam 2200529 450 001 9910136805903321 005 20230808192403.0 035 $a(CKB)3710000000631086 035 $a(oapen)https://directory.doabooks.org/handle/20.500.12854/41045 035 $a(EXLCZ)993710000000631086 100 $a20160411c2016uuuu fy| 0 101 0 $aeng 135 $aurc|#---||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aApplication of genetically encoded indicators to mammalian central nervous system$b[electronic resource] /$fedited by Yoshiyuki Yamada and Katsuhiko Mikoshiba 210 $cFrontiers Media SA$d2016 210 1$a[Place of publication not identified] :$cFrontiers Media SA,$d2016. 215 $a1 online resource (116 pages) $cillustrations; digital file(s) 225 0 $aFrontiers Journal series 225 1 $aFrontiers Research Topics,$x1664-8714 300 $a"Published in: Frontiers in molecular neuroscience" --front cover. 311 $a2-88919-804-9 320 $aIncludes bibliographical references. 327 $aEditorial: application of genetically encoded indicators to mammalian central nervous system --Monitoring activity in neural circuits with genetically encoded indicators --Putting a finishing touch on GECIs --Genetically encoded CaČ? indicators; expanded affinity range, color hue and compatibility with optogenetics --Investigating neuronal function with optically controllable proteins --Exploration of genetically encoded voltage indicators based on a chimeric voltage sensing domain --Engineering a genetically-encoded SHG chromophore by electrostatic targeting to the membrane --Imaging activity in astrocytes and neurones with genetically encoded calcium indicators following in utero electroporation --Intracellular calcium dynamics in cortical microglia responding to focal laser injury in the PC::G5-tdT reporter mouse --Wide-field CaČ? imaging reveals visually evoked activity in the retrosplenial area --Habituation of glomerular responses in the olfactory bulb following prolonged odor stimulation reflects reduced peripheral input. 330 3 $aGenetically encoded indicators emerged as promising tools for cell type-specific and chronic recording of neuronal population activity. Since publication of the first prototypical genetically encoded Ca2+ indicators (Cameleons) in 1997, we have witnessed remarkable evolution of the field, with rapid improvement of indicator performance as well as expanded application to many model organisms in the neuroscience community. Challenges still remain, however, concerning the mammalian central nervous system: limited sensitivity of indicators to subtle changes in activity, slow signal kinetics, cytotoxicity after a long-term and high-level expression of indicators, and variable performance across cell types. In addition to improvement of the indicators per se, development of strategies that allow combined use of the indicators and optogenetic tools is also desired. In this Research Topic, we recruited top researchers in the field and their young colleagues to present their cutting-edge research as well as insightful opinions on the following subtopics:1) Latest breakthroughs on development of genetically encoded indicators2) Novel scientific findings obtained with genetically encoded indicators3) Wishlist for the next-generation genetically encoded indicators4) Guideline for selecting an appropriate indicator5) Optimal methodology for indicator delivery to mammalian CNS. 410 0$aFrontiers Research Topics,$x1664-8714 606 $aNeurosciences 606 $aNeurosciences$xResearch 606 $aNeuropsychiatry$xResearch 610 $ain vivo imaging 610 $aGenetically encoded calcium indicators (GECIs) 610 $agenetically encoded voltage sensor 610 $aFluorescent protein sensors 610 $agenetically encoded sensors 615 0$aNeurosciences. 615 0$aNeurosciences$xResearch. 615 0$aNeuropsychiatry$xResearch. 700 $aYoshiyuki Yamada$4auth$01376542 702 $aYamada$b Yoshiyuki 702 $aMikoshiba$b Katsuhiko$f1945- 801 2$bUkMaJRU 906 $aBOOK 912 $a9910136805903321 996 $aApplication of genetically encoded indicators to mammalian central nervous system$93412437 997 $aUNINA