LEADER 04624nam 2200601 450 001 9910136458303321 005 20230808192206.0 010 $a3-527-80132-4 010 $a3-527-80129-4 035 $a(CKB)3710000000616929 035 $a(EBL)4451921 035 $a(MiAaPQ)EBC4451921 035 $a(Au-PeEL)EBL4451921 035 $a(CaPaEBR)ebr11172365 035 $a(CaONFJC)MIL909459 035 $a(OCoLC)944934602 035 $a(EXLCZ)993710000000616929 100 $a20160324h20162016 uy| 0 101 0 $aeng 135 $aur|n|---||||| 181 $2rdacontent 182 $2rdamedia 183 $2rdacarrier 200 00$aMicro- and nanosystems for biotechnology /$fedited by J. Christopher Love 210 1$aWeinheim, Germany :$cWiley-VCH Verlag GmBH & Company KGaA,$d[2016] 210 4$dİ2016 215 $a1 online resource (305 p.) 225 0 $aAdvanced biotechnology ;$vvolume 2 300 $aDescription based upon print version of record. 311 $a3-527-80131-6 311 $a3-527-33281-2 320 $aIncludes bibliographical references and index. 327 $aCover; Title Page; Copyright; Contents; List of Contributors; About the Series Editors; Preface; Part I Microsystems for Single-Cell Analysis; Chapter 1 Types of Clinical Samples and Cellular Enrichment Strategies; 1.1 Introduction; 1.2 Types of Clinical Samples; 1.2.1 Solid Clinical Samples; 1.2.1.1 Cellular Subtypes Found in Solid Clinical Samples; 1.2.2 Liquid Clinical Samples and Cellular Subtypes; 1.2.2.1 Blood; 1.2.2.2 Bone Marrow; 1.2.2.3 Placental or Umbilical Cord Blood; 1.2.2.4 Urine; 1.2.2.5 Cerebrospinal Fluid (CSF); 1.2.2.6 Saliva 327 $a1.3 Sample Processing and Conventional Methods of Cell Enrichment1.3.1 Processing Solid Clinical Samples; 1.3.1.1 Processing Liquid Samples; 1.3.2 Cell Enrichment; 1.3.2.1 Laser Capture Microdissection (LCM); 1.3.2.2 Density Gradient Centrifugation; 1.3.2.3 Fluorescence-Activated Cell Sorting (FACS); 1.3.2.4 Magnetic Activated Cell Sorting (MACS); 1.3.2.5 CellSearchTM; 1.4 Microscale/Nanoscale Devices for Cellular Enrichment; 1.4.1 Filtration Approaches; 1.4.2 Hydrodynamic Mechanisms; 1.4.3 Surface Treatments; 1.4.4 Magnetophoresis; 1.4.5 Electrophoresis; 1.4.6 Acoustophoresis 327 $a1.4.7 Optical Tweezers/Traps1.5 Conclusion; References; Chapter 2 Genome-Wide Analysis of Single Cells and the Role of Microfluidics; 2.1 Motivation for Single-Cell Analysis of Genomes and Transcriptomes; 2.2 Single-Cell Genomics; 2.2.1 Major Technical Challenges; 2.2.2 Approaches to Single-Cell Genomics; 2.2.3 The Application and Impact of Microfluidics in Single-Cell Genomics; 2.3 Single-Cell Transcriptomics; 2.3.1 Major Technical Challenges; 2.3.2 Approaches to Single-Cell Transcriptomics; 2.3.3 Application and Impact of Microfluidics in Single-Cell Transcriptomics 327 $a2.4 The Future of Genome-Wide Single-Cell Analysis with Microfluidics2.4.1 Recent Advances in the Scalability of Single-Cell Analysis using Microfluidics; 2.4.2 How Microfluidics will Expand the Application-Space for Single-Cell Analysis; 2.4.3 Outstanding Hurdles for Genome-Wide Analysis of Single Cells; 2.4.4 Prospects for Clinical Applications of Microfluidic Single-Cell Analysis; Keywords and Definitions; References; Chapter 3 Cellular Immunophenotyping: Industrial Technologies and Emerging Tools; 3.1 Cellular Immune Status and Immunophenotyping; 3.2 Surface Marker Phenotyping 327 $a3.2.1 Multicolor Flow Cytometry3.2.2 Commercial Flow Cytometers; 3.2.3 High-Content Imaging Cytometry; 3.2.4 Current Limitations and Further Development of Flow Cytometry; 3.3 Functional Phenotyping; 3.3.1 ELISpot Technologies; 3.3.2 Multiplexed Immunoassays; 3.3.3 Emerging Single-Cell Technologies; 3.4 Conclusion; Keywords and Definitions; References; Chapter 4 Microsystem Assays for Studying the Interactions between Single Cells; 4.1 Introduction; 4.2 Advantages of Single-Cell Analysis over Conventional Assay Systems; 4.3 Analysis of Cell-Cell Communication between Pairs of Single Cells 327 $a4.3.1 Integrated Microfluidic Coculture Systems and Microwell Arrays 410 0$aAdvanced Biotechnology 606 $aBiotechnology 606 $aNanotechnology 606 $aMicrotechnology 615 0$aBiotechnology. 615 0$aNanotechnology. 615 0$aMicrotechnology. 702 $aLove$b J. Christopher 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910136458303321 996 $aMicro- and nanosystems for biotechnology$91915557 997 $aUNINA