LEADER 02904nam 2200601 450 001 9910136278103321 005 20230621135812.0 010 $a9782889194551 (ebook) 035 $a(CKB)3710000000586920 035 $a(SSID)ssj0001680126 035 $a(PQKBManifestationID)16496184 035 $a(PQKBTitleCode)TC0001680126 035 $a(PQKBWorkID)15028316 035 $a(PQKB)10699518 035 $a(WaSeSS)IndRDA00057299 035 $a(oapen)https://directory.doabooks.org/handle/20.500.12854/45325 035 $a(EXLCZ)993710000000586920 100 $a20160829d2015 uy | 101 0 $aeng 135 $aur||||||||||| 181 $ctxt 182 $cc 183 $acr 200 00$aDNA polymerases in biotechnology$b[electronic resource] /$ftopic editors Andrew F. Gardner and Zvi Kelman 210 $cFrontiers Media SA$d2015 210 31$aFrance :$cFrontiers Media SA,$d2015 215 $a1 online resource (146 pages) $cillustrations, charts 225 0 $aFrontiers Research Topics 300 $aBibliographic Level Mode of Issuance: Monograph 320 $aIncludes bibliographical references. 330 $aDNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology. For example, DNA polymerases have been engineered for increased speed and fidelity in PCR while lowering amplification sequence bias. Inhibitor resistant DNA polymerase variants enable PCR directly from tissue (i.e. blood). Design of DNA polymerases that efficiently incorporate modified nucleotide have been critical for development of next generation DNA sequencing, synthetic biology and other labeling and detection technologies. The Frontiers in Microbiology Research Topic on DNA polymerases in Biotechnology aims to capture current research on DNA polymerases and their use in emerging technologies. 606 $aAnimal Biochemistry$2HILCC 606 $aHuman Anatomy & Physiology$2HILCC 606 $aHealth & Biological Sciences$2HILCC 610 $aDNA polymerase evolution 610 $aDNA polymerase fidelity 610 $amolecular diagnostics 610 $aDNA polymerase 610 $aPCR inhibitors 610 $anext generation sequencing 610 $aPCR 610 $aDNA sequencing 615 7$aAnimal Biochemistry 615 7$aHuman Anatomy & Physiology 615 7$aHealth & Biological Sciences 700 $aZvi Kelman$4auth$01365472 702 $aKelman$b Zvi 702 $aGardner$b Andrew F 801 0$bPQKB 801 2$bUkMaJRU 912 $a9910136278103321 996 $aDNA polymerases in biotechnology$93387411 997 $aUNINA