LEADER 05508nam 2200721Ia 450 001 9910133840603321 005 20200520144314.0 010 $a1-283-83535-5 010 $a3-527-63820-2 010 $a3-527-63821-0 010 $a3-527-63819-9 035 $a(CKB)3340000000000193 035 $a(EBL)822728 035 $a(OCoLC)787842600 035 $a(SSID)ssj0000597326 035 $a(PQKBManifestationID)11378994 035 $a(PQKBTitleCode)TC0000597326 035 $a(PQKBWorkID)10577604 035 $a(PQKB)10539896 035 $a(OCoLC)759212293 035 $a(MiAaPQ)EBC822728 035 $a(MiAaPQ)EBC4042256 035 $a(Au-PeEL)EBL822728 035 $a(CaPaEBR)ebr10577683 035 $a(CaONFJC)MIL414785 035 $a(PPN)194575764 035 $a(EXLCZ)993340000000000193 100 $a20140721d2011 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aBSL3 and BSL4 agents$b[electronic resource] $eproteomics, glycomics, and antigenicity /$fedited by Jiri Stulik ... [et al.] 210 $aWeinheim $cWiley-Blackwell$dc2011 215 $a1 online resource (258 p.) 300 $aDescription based upon print version of record. 311 $a3-527-32780-0 320 $aIncludes bibliographical references and index. 327 $aBSL3 and BSL4 Agents: Proteomics, Glycomics, and Antigenicity; Contents; Preface; List of Contributors; 1: Introduction: Application of Proteomic Technologies for the Analysis of Microbial Infections; 1.1 Introduction; 1.2 Search for New Factors of Virulence and Potential Diagnostic Markers; 1.3 Search for New Vaccine Candidates; 1.4 Analysis of Post-Translational Modifications of Bacterial Proteins and Protein-Protein Interactions; 1.5 Conclusions; References; Part One: Basic Proteomic Methods; 2: Separation of Proteins and Peptides; 2.1 Introduction; 2.1.1 Gel-Based Separation 327 $a2.1.1.1 One-Dimensional Electrophoresis2.1.1.2 Two-Dimensional Electrophoresis; 2.1.1.3 Protein Staining and Image Analysis; 2.1.1.4 2-DE Limitations; 2.1.2 In Solution-"Gel Free" Proteomics; 2.1.3 Column Chromatography; 2.1.3.1 Size Exclusion Chromatography; 2.1.3.2 Reversed-Phase Liquid Chromatography; 2.1.3.3 Hydrophilic Interaction Liquid Chromatography; 2.1.3.4 Ion Exchanger Chromatography; 2.1.3.5 Affinity Chromatography; 2.1.3.6 Multidimensional Chromatography; 2.1.4 Liquid Phase IEF and Electrophoresis; 2.1.5 Alternative Separation Technologies; Acknowledgment; References 327 $a3: Basic Mass Spectrometric Approaches3.1 Introduction; 3.2 Ionization; 3.2.1 Matrix-Assisted Laser Desorption/Ionization; 3.2.2 Electrospray Ionization; 3.3 Mass Analyzers; 3.3.1 Time of Flight; 3.3.2 Reflectron TOF; 3.3.3 Quadrupole and Ion Trap; 3.3.4 Fourier Transformation Ion Cyclotron; 3.3.5 Tandem Mass Analyzers; 3.3.6 Ion Detection; 3.4 Protein Identification; 3.4.1 Combination of 2-DE and MS; 3.4.2 Peptide Mass Fingerprinting; 3.4.3 Peptide Sequencing (PMF); 3.4.4 Shotgun Proteomics; 3.5 Conclusion; Acknowledgments; References; 4: Quantitative Mass Spectrometric Approaches 327 $a4.1 Introduction4.1.1 Gel-Based Quantitative Proteomic Methods; 4.1.2 Shotgun Quantitative Proteomic Methods; 4.1.3 Labeling Methods; 4.1.3.1 Metabolic Incorporation of Stable Isotopes; 4.1.3.2 Enzymatic Incorporation of Stable Isotopes; 4.1.3.3 Chemical Incorporation of Stable Isotopes; 4.2 iTRAQ Analysis of Bacterial Pathogens; 4.2.1 Bacterial Cell Disruption and Protein Extraction; 4.2.2 Determination of Protein Concentration; 4.2.3 Protein Digestion; 4.2.4 Peptide Labeling with iTRAQ Tags; 4.2.5 Protocol for iTRAQ Analysis of Bacterial Proteins; References 327 $a5: BN-PAGE of Microbial Protein Complexes5.1 Introduction; 5.2 Methods for Studying Protein-Protein Interactions; 5.3 Blue Native Polyacrylamide Gel Electophoresis; 5.3.1 Sample Preparation; 5.3.1.1 Non-Denaturing Conditions; 5.3.1.2 Selection of Detergent and Its Optimal Concentration; 5.3.1.3 Membrane and Cytosolic Fraction Separation; 5.3.2 1D BN-PAGE; 5.3.3 2D BN/SDS-PAGE; 5.4 Evaluation of BN-PAGE-Staining, MS, Western Blotting; 5.4.1 Staining; 5.4.1.1 Silver Staining; 5.4.1.2 Fluorescent Staining; 5.4.1.3 Coomassie Staining; 5.4.2 Mass Spectrometry; 5.4.3 Western Blotting 327 $a5.4.4 Other Methods of Visualization 330 $aUnique coverage of proteomic and glycomic approaches to better distinguish highly dangerous pathogens, as well as using these to explore novel treatment and prevention options. The editors and authors are either part of a specialized European network initiated to develop fast and reliable detection and therapy options, or are associated with the core military research complex of the United States.With its description of the methods, their advantages and limitations, as well as the principle outcomes, this is a must-have resource for all professionals dealing with BSL3 and/or BSL 4 agen 606 $aPathogenic microorganisms$xAnalysis 606 $aProteomics 606 $aGlycomics 606 $aAntigens 615 0$aPathogenic microorganisms$xAnalysis. 615 0$aProteomics. 615 0$aGlycomics. 615 0$aAntigens. 676 $a579.165 701 $aS?tulik$b J?iri?$0939837 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910133840603321 996 $aBSL3 and BSL4 agents$92119010 997 $aUNINA