LEADER 02923nam 2200649 450 001 9910132350203321 005 20230807212034.0 010 $a1-4443-3695-9 010 $a1-118-87611-3 010 $a1-118-87500-1 010 $a1-118-87571-0 035 $a(CKB)3710000000251862 035 $a(EBL)1811632 035 $a(SSID)ssj0001347675 035 $a(PQKBManifestationID)11776006 035 $a(PQKBTitleCode)TC0001347675 035 $a(PQKBWorkID)11370663 035 $a(PQKB)11345335 035 $a(MiAaPQ)EBC1811632 035 $a(DLC) 2014000681 035 $a(Au-PeEL)EBL1811632 035 $a(CaPaEBR)ebr10951343 035 $a(CaONFJC)MIL650887 035 $a(OCoLC)892799535 035 $a(EXLCZ)993710000000251862 100 $a20141017h20152015 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aInsect histology $epractical laboratory techniques /$fPedro Barbosa, Deborah L. Berry, Christina S. Kary 205 $aSecond edition. 210 1$aWest Sussex, England :$cJohn Wiley & Sons,$d2015. 210 4$dİ2015 215 $a1 online resource (397 p.) 300 $aDescription based upon print version of record. 311 $a1-322-19607-9 311 $a1-4443-3696-7 320 $aIncludes bibliographical references and index. 327 $aProblems of sclerotized chitin : softening insect cuticle -- Fixation -- Dehydration, clearing, and embedding -- Staining -- Immunohistochemical staining -- Use of genetic markers in insect histology -- Fluorescence -- Mounting -- Preparation of whole mounts -- Whole mount staining -- Preparation of insect tissues -- Preparation of genitalia, mouthparts, and other body parts -- Preparation of chromosomes -- Appendix : dissecting fluids and insect saline solutions. 330 $aThis title is a much needed update of Barbosa''s self-published Manual of Basic Techniques in Insect Histology. It is a laboratory manual of ''traditional'' and ''modern'' insect histology techniques, completely revised using cutting-edge methodology carried out today and includes new immunohistochemical techniques not previously looked at. Insect Histology is designed as a resource for student and professional researchers, in academia and industry, who require basic information on the procedures that are essential for the histological display of the tissues of insects and related organisms. 606 $aEntomology$vLaboratory manuals 606 $aHistology$vLaboratory manuals 615 0$aEntomology 615 0$aHistology 676 $a595.7 700 $aBarbosa$b Pedro$f1944-$0916242 702 $aBerry$b Deborah L. 702 $aKary$b Christina S. 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910132350203321 996 $aInsect histology$92184915 997 $aUNINA