LEADER 05503nam 2200733 450 001 9910132186803321 005 20230803203826.0 010 $a1-118-33611-9 010 $a1-118-33614-3 010 $a1-118-33613-5 035 $a(CKB)3710000000204404 035 $a(EBL)1753758 035 $a(OCoLC)885019005 035 $a(SSID)ssj0001288363 035 $a(PQKBManifestationID)12443341 035 $a(PQKBTitleCode)TC0001288363 035 $a(PQKBWorkID)11294064 035 $a(PQKB)11390816 035 $a(PQKBManifestationID)16046358 035 $a(PQKB)20392911 035 $a(OCoLC)889316924 035 $a(MiAaPQ)EBC1753758 035 $a(DLC) 2014022003 035 $a(Au-PeEL)EBL1753758 035 $a(CaPaEBR)ebr10902335 035 $a(CaONFJC)MIL632093 035 $a(OCoLC)881064963 035 $a(EXLCZ)993710000000204404 100 $a20140815h20142014 uy 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt 182 $cc 183 $acr 200 00$aBiofouling methods /$fedited by Sergey Dobretsov, Jeremy C. Thomason, David N. Williams 205 $aFirst edition. 210 1$aWest Sussex, England :$cJohn Wiley & Sons, Ltd.,$d2014. 210 4$dİ2014 215 $a1 online resource (411 p.) 300 $aIncludes index. 311 $a0-470-65985-8 320 $aIncludes bibliographical references and index. 327 $aBiofouling Methods; Copyright; Contents; List of contributors; Introduction; Guide to methods; Part I Methods for Microfouling ; Chapter 1 Microscopy of biofilms; Section 1: Traditional light and epifluorescent microscopy; 1.1 Introduction; 1.2 Determination of bacterial abundance; 1.3 Catalyzed reporter deposition fluorescent in situ hybridization (CARD-FISH); 1.4 Suggestions, with examples, for data analysis and presentation; Acknowledgements; References; Chapter 2 Traditional and bulk methods for biofilms; Section 1: Traditional microbiological methods; 2.1 Introduction 327 $a2.2 Enrichment culture, isolation of microbes 2.3 Counting methods; 2.4 Troubleshooting hints and tips; References; Section 2 Bulk methods; 2.5 Introduction; 2.6 Measurement of biofilm thickness; 2.7 Biofilm dry weight determination; 2.8 Biofilm ATP content; 2.9 Troubleshooting hints and tips; Acknowledgements; References; Chapter 3 Biocide testing against microbes; Section 1: Testing biocides in solution: flow cytometry for planktonic stages; 3.1 Introduction; 3.2 Method introductions; 3.3 Pros and cons; 3.4 Materials and equipment; 3.5 Methods; 3.6 Troubleshooting hints and tips 327 $a3.7 Suggestions References; Section 2 Biocide testing using single and multispecies biofilms; 3.8 Introduction; 3.9 Questions to answer when applying biocides; 3.10 Laboratory methods for testing biocide effect; 3.11 Field methods for testing biocide effect; 3.12 Troubleshooting hints and tips; Acknowledgements; References; Chapter 4 Molecular methods for biofilms; Section 1: Isolation of nucleic acids; 4.1 Introduction; 4.2 Materials; 4.3 Isolation of DNA from a biofilm; 4.4 Troubleshooting hints and tips; References; Section 2 PCR and DNA sequencing 327 $a4.5 PCR and DNA sequencing: General introduction 4.6 PCR; 4.7 Microbial marker genes - 16S; 4.8 DNA sequencing; 4.9 454 16S amplicon pyrotag sequencing; 4.10 Protocol 1: DNA extraction using the Qiagen DNeasy Plant Mini Kit; 4.11 Protocol 2: Full-length 16S PCR using the Qiagen Multiplex Kit; 4.12 Protocol 3: Analysis of full-length 16S genes; 4.13 Protocol 4: 16S amplicon PCR for 454 sequencing using the Qiagen Multiplex Kit; 4.14 Protocol 5: Trimming and filtering of 454 16S pyrotag sequencing; 4.15 Protocol 6: Taxon-based analyses; 4.16 Protocol 7: Phylogeny-based analyses; References 327 $aSection 3 Community comparison by genetic fingerprinting techniques 4.17 Introduction; 4.18 History and principles of the methods; 4.19 Advantages and limitations of fingerprinting techniques; 4.20 Materials and equipment; 4.21 Suggestions for data analysis and presentation; 4.22 Troubleshooting hints and tips; Acknowledgements; References; Section 4 Metagenomics; 4.23 Introduction and brief summary of methods; 4.24 Overview of metagenomics methods; 4.25 Method introduction; 4.26 Overview of DNA handling for BAC library construction; 4.27 BAC and Fosmid library construction 327 $a4.28 Library handling, archiving, and databasing 330 $aBiofouling Methods provides a "cook book" for both established workers and those new to the field. The methods included in this important new book range from tried and tested techniques to those at the cutting edge, encompassing the full diversity of this multidisciplinary field. The book covers methods for microbial and macrofouling, coatings and biocides, and ranges from methods for fundamental studies to methods relevant for industrial applications. There is an emphasis on answering questions and each chapter provides technical methods and problem-solving hints and tips. Bringing togethe 606 $aFouling 606 $aFouling organisms 615 0$aFouling. 615 0$aFouling organisms. 676 $a628.9/6 702 $aDobretsov$b Sergey 702 $aThomason$b Jeremy C. 702 $aWilliams$b David N. 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910132186803321 996 $aBiofouling methods$92259738 997 $aUNINA