LEADER 03882nam 22007455 450 001 9910131313103321 005 20240529145243.0 010 $a3-319-16259-4 024 7 $a10.1007/978-3-319-16259-1 035 $a(CKB)3710000000415186 035 $a(SSID)ssj0001500656 035 $a(PQKBManifestationID)11967966 035 $a(PQKBTitleCode)TC0001500656 035 $a(PQKBWorkID)11535914 035 $a(PQKB)10488240 035 $a(DE-He213)978-3-319-16259-1 035 $a(MiAaPQ)EBC3565663 035 $a(MiAaPQ)EBC6422689 035 $a(Au-PeEL)EBL6422689 035 $a(OCoLC)1231605307 035 $a(PPN)186026102 035 $a(EXLCZ)993710000000415186 100 $a20150508d2015 u| 0 101 0 $aeng 135 $aurnn#008mamaa 181 $ctxt 182 $cc 183 $acr 200 10$aLow-Cost Methods for Molecular Characterization of Mutant Plants $eTissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /$fby Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger 205 $a1st ed. 2015. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2015. 215 $a1 online resource (X, 35 p. 9 illus., 3 illus. in color.) 300 $aBibliographic Level Mode of Issuance: Monograph 311 $a3-319-16258-6 327 $aIntroduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.  . 330 $aThis book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits. 606 $aPlant biotechnology 606 $aBiology?Technique 606 $aBiomaterials 606 $aNucleic acids 606 $aPlant Biotechnology 606 $aBiological Techniques 606 $aNucleic Acid 615 0$aPlant biotechnology. 615 0$aBiology?Technique. 615 0$aBiomaterials. 615 0$aNucleic acids. 615 14$aPlant Biotechnology. 615 24$aBiological Techniques. 615 24$aNucleic Acid. 676 $a631.52 676 $a660.6 700 $aTill$b Bradley J$4aut$4http://id.loc.gov/vocabulary/relators/aut$0993843 702 $aJankowicz-Cieslak$b Joanna$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aHuynh$b Owen A$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aBeshir$b Mayada M$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aLaport$b Robert G$4aut$4http://id.loc.gov/vocabulary/relators/aut 702 $aHofinger$b Bernhard J$4aut$4http://id.loc.gov/vocabulary/relators/aut 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910131313103321 996 $aLow-Cost Methods for Molecular Characterization of Mutant Plants$92275613 997 $aUNINA